Preparation method and application of hydrogel based on rhabdovirus

Abstract

The invention discloses a preparation method of hydrogel based on rhabdovirus. The virus hydrogel is composed of macromolecule-modified rod-shaped composite virus particles, and the rhabdovirus is fd bacteriophage. The preparation method comprises the following steps of: preparing a thermo-sensitive polymer solution; preparing a virus solution of the fd bacteriophage; dropwise adding the thermo-sensitive polymer solution into the virus solution to obtain thermo-sensitive macromolecule-modified composite virus particles; and dissolving the polymer-modified virus particles in a phosphoric acid buffer solution to obtain a solution, and heating the solution to a temperature higher than the gelation transformation temperature to obtain the hydrogel based on rhabdovirus. The preparation method disclosed by the invention has the advantages that the virus hydrogel has good biocompatibility and can generate quick and reversible sol-gel transformation at a specific gelation temperature point; massive chemical functional groups on the surface of the rhabdovirus can be bound with specific-function groups as chemical reaction hot spots; and after gel formation, the active groups on the skeleton surface are still available, and the hydrogel can be used as an insulin sustained-release intelligent material.

Description

technical field [0001] The invention relates to the field of polymer materials, in particular to a preparation method and application of a baculovirus-based hydrogel. Background technique [0002] Hydrogel is a kind of network system that uses water as the dispersion medium and is hydrophilic but insoluble in water. Because the three-dimensional network structure similar to solid contains abundant water, it is widely used in medicine, biology, materials, food, cosmetics and people's daily life. There are very important applications in life. Common hydrogel framework materials are mostly polymers and supramolecular self-assemblies. The hydrogel with polymer as the skeleton forms a three-dimensional network structure through physical or chemical crosslinking, and confines water molecules inside it. Hydrogels formed based on the self-assembly of small organic molecules or protein polypeptides are also frequently reported. This type of hydrogel with a supramolecular assembly ...

AI Technical Summary

Problems solved by technology

[0003] Although the existing hydrogels already have many characteristics such as high mechanical strength, good stimulus-response, injectability, biocompatibility, etc., usually a hydrogel only contains one or a small amount of these advantages, Overall, there are still many shortcomings that limit the development and application of hydrogels

For example, solvent residues or cross-linking agents used in the polymerization process of hydrogels based on polymer backbones may be biologically toxic, and the reversibility of gelation is poor; while supramolecular self-assembled scaffolds need to be assembled into nanofibers from small molecules first, and the steps are cumbersome.

The chemical remodification of many hydrogel three-dimensional network skeletons is poor, which is not conducive to the multifunctional design of hydrogels

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