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Aspergillus niger and application thereof

A technology of Aspergillus niger and lipase, which is applied in the field of active bacteria that efficiently decompose fat, can solve the problems of harsh reaction conditions, toxic by-products, environmental pollution, etc., and achieve the effect of not being prone to by-products, strong specificity, and environmental friendliness

Inactive Publication Date: 2013-09-25
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, most esterification reactions are catalyzed by high-temperature acid and alkali, and there are problems such as toxic by-products, harsh reaction conditions, high energy consumption, and environmental pollution.

Method used

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  • Aspergillus niger and application thereof
  • Aspergillus niger and application thereof
  • Aspergillus niger and application thereof

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Embodiment Construction

[0008] The present invention isolates a strain with high lipase-producing activity from the oil-rich soil and water samples near the sewer near the canteen and the restaurant. According to biological and molecular identification, the strain Yz15 is in the same branch as Aspergillus niger strain CBS 513.88clone F-wpysw4 (EU710739.1), and the sequence homology is 100%. According to the morphological judgment combined with 18SrDNA sequence analysis, the strain Yz15 was consistent with the morphological characteristics of Aspergillus niger in the genus Aspergillus. Yz15 was identified as Aspergillus niger, numbered Yz15No.110411-3. The morphological characteristics of the culture medium: the colony grows rapidly on the PDA medium, and can cover the entire petri dish with a diameter of 9cm after being cultured at 28°C for 5 days. , no exudate, and the opposite side of the colony was orange-yellow in varying degrees. Colonies grow slowly on lipid media. Its microscopic morphologi...

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Abstract

The invention provides aspergillus niger and an application thereof. The aspergillus niger has an ability of decomposition of cellulose and lignin. Microscope morphological characteristics of the aspergillus niger are that: a conidia head is radial in shape, the diameter is about 200 [mu]m, and a conidiophore stem is about 2 mm; a top sac is nearly spherical in shape, the diameter of the top sac is 20 to 30 [mu]m, and a conidia is spherical in shape and black brown; an optimum temperature of a lipase of the aspergillus niger is 40 DEG C, and has good stability at a temperature within 35 DEG C; and the lipase of the aspergillus nigerin has good stability in a pH value range of 5 to 9.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an active bacterium for decomposing fat with high efficiency and the use of the bacterium. Background technique [0002] Lipase is one of the important industrial enzyme preparations, which can catalyze lipolysis, transesterification, ester synthesis and other reactions, and is widely used in oil processing, food, medicine, daily chemical and other industries. Lipases from different sources have different catalytic characteristics and catalytic activities. Application of lipase 1) The food industry uses the fatty acids with shorter chains released after enzyme action to increase and improve the flavor and aroma of food; use lipase-catalyzed alcoholysis and esterification reactions to produce various flavor esters as seasonings agent; use 1,3-specific microbial lipase to improve the nutritional value of edible oil and increase the variety of edible oil to prepare high-gra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/685
Inventor 赵萍王雅陆届瑾
Owner LANZHOU UNIVERSITY OF TECHNOLOGY