Breeding, multiplying and seed production methods for non-heading cabbage cytoplasmic male sterility line
A technology of male sterile line and head cabbage, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve problems such as sterility instability, nectary degeneration, etc. Simple, high-purity effects
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Embodiment 1
[0020] Example 1 Breeding of non-heading Chinese cabbage cytoplasmic male sterile line
[0021] (1) Using hau CMS, a cytoplasmic male sterile material of Brassica juncea (hau CMS, which has been disclosed in the Chinese invention patent application number 200510086942.7, and the seed has been preserved in the China Type Culture Collection Center on November 12, 2005, and the preservation number is: CCTCC NO: P200504) was used as the female parent, and the maintainer line "Aijiaohuang" (the seeds of this breeding material have been sold publicly before the application date) was used as the male parent for distant hybridization, cultivated by embryo rescue method, and transplanted in the field to obtain the interspecific hybrid F 1 ;
[0022] (2) The interspecific hybrid F obtained by step (1) 1 The female parent was crossed with the maintainer non-heading Chinese cabbage "Aijiaohuang", and the non-heading Chinese cabbage BC1F was obtained by isolated embryo culture 1 ;
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Embodiment 2
[0036] Example 2 Propagation and seed production of non-heading Chinese cabbage cytoplasmic male sterile line 98-1A
[0037] Under the isolation conditions of autumn sowing in Wuhan, Hubei, China (September every year) or summer sowing in Lanzhou, Gansu, China (May every year), the non-heading Chinese cabbage cytoplasmic male sterile line 98-1A was used as the female parent, "Dwarf yellow" As the male parent, the male parent: female parent is 2:4 row ratio (ratio of the number of rows) to propagate the non-heading Chinese cabbage cytoplasmic male sterile line 98-1A, and other cultivation management is the same as that of common non-header Chinese cabbage. Breed the same.
[0038] The non-heading Chinese cabbage cytoplasmic male sterile line 98-1A was crossed with "Shanghai Qing" to obtain the non-heading Chinese cabbage cytoplasmic male sterile hybrid variety.
[0039] Table 1 Comparison of phenotypes between non-heading Chinese cabbage cytoplasmic male sterile line 98-1A and...
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