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Method for detecting human immunoglobulin E by adopting carbon nano tube micro-cantilever biosensor

A biosensor and immunoglobulin technology, which is applied in the field of detecting hIgE with micro-cantilever biosensors, can solve the problems of complex operation, low sensitivity, and difficulty in realizing early disease diagnosis and research.

Inactive Publication Date: 2013-10-02
GUILIN UNIV OF ELECTRONIC TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Among them, radioimmunoassay has radiation and is not safe; other detection methods are complicated to operate and have low sensitivity, which makes it difficult to realize early diagnosis and research of diseases, and cannot meet the needs of rapid detection.

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  • Method for detecting human immunoglobulin E by adopting carbon nano tube micro-cantilever biosensor

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Effect test

Embodiment 1

[0024] The present invention detects the steps of hIgE as follows:

[0025] (1) Place the carbon nanotube micro-cantilever in a solution containing a nucleic acid aptamer that has a specific recognition effect on hIgE, and modify the nucleic acid aptamer on the carbon nanotube by ultrasonic treatment, and the modified The method can be that the nucleic acid aptamer is modified on the carbon nanotube through hydrophobic interaction, or can be modified on the carbon nanotube through π-π superimposition to form a detection probe containing hIgE nucleic acid aptamer;

[0026] (2) Drop the sample to be tested onto the carbon nanotube microcantilever modified with nucleic acid aptamers, and incubate at room temperature for 15 minutes, so that the nucleic acid aptamer on the biosensing interface and the h in the sample to be tested IgE undergoes a specific recognition reaction and forms a complex;

[0027] (3) The mass size of the formed complex is positively correlated with the con...

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Abstract

The invention discloses a detection method which is high in sensitivity, fast and accurate and can rapidly measure human immunoglobulin E (hIg E). The method is realized by constructing a carbon nano tube micro-cantilever biosensor. The biosensor comprises a bracket, a substrate material, a carbon nano tube and a pick-up circuit, wherein an aptamer layer is modified on the carbon nano tube. The method comprises the following steps of: manufacturing a detection probe containing a hIg E aptamer on a carbon nano tube micro-cantilever, putting the detection probe into a sample to be detected during detection, forming a compound between the hIg E in the sample to be detected and the aptamer on the detection probe through a specific reaction and attaching the compound to the micro-cantilever; and detecting the hIg E by utilizing change relationship of deflection displacement or resonant frequency of the micro-cantilever caused by mass change of the compound generated on the micro-cantilever and positive correlation between the mass size of the compound and the concentration of the hIg E in the sample to be detected.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a method for detecting hIgE with a micro-cantilever beam biosensor. technical background [0002] Human immunoglobulin E (h IgE) is a common tumor marker. Currently, there are many methods for detecting h IgE, including radioimmunoassay, enzyme-linked immunoassay, and fluorescent immunoassay. Among them, the radioimmunoassay method has radiation and is not safe; other detection methods are complicated to operate and have low sensitivity, making it difficult to realize the diagnosis and research of early diseases, and cannot meet the needs of rapid detection. It is necessary to establish a rapid, sensitive and easy-to-operate hIgE detection method. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a detection method with high sensitivity, simple structure, fast and accurate, which can realize rapid determination of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
CPCG01N5/00G01N33/6854
Inventor 黄勇梁晋涛李桂银周治德黄国银管明源马龙飞
Owner GUILIN UNIV OF ELECTRONIC TECH
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