Method for identifying resistance function of aphid-resisting genes through transforming hairy roots of soybean
A technology of aphid-resistant gene and hairy root, which is applied in the field of identifying the resistance function of the aphid-resistant gene by transforming soybean hairy root, can solve problems such as difficult control of aphids, and achieve simple and convenient implementation, accurate identification, convenient and reliable identification Effect
Inactive Publication Date: 2013-10-09
SHANGHAI JIAO TONG UNIV
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Abstract
The invention discloses a method for identifying the resistance function of aphid-resisting genes by transforming hairy roots of soybean and relates to the field of methods for crop tissue culture and transgenosis. According to the method, the hairy roots are cultured by infecting mature seeds of the soybean by agrobacterium rhizogenes, and the effect of the aphid-resisting genes to resist against soybean aphids is verified through the transgenic hairy roots. The method mainly includes a cotyledon obtaining and culturing technique for the mature seeds of the soybean, an infection transformation technique for the agrobacterium rhizogenes, a degerming and continuous culturing technique for the transgenic hairy roots, a transgenic hairy root aphid inoculation technique, and an identification technique for the aphid-resisting functional genes of the hairy roots. The method is simple, easy to operate, free of limitation of seasons and capable of effectively and quickly identifying the effect of the target genes to resist against the soybean aphids through the transgenic hairy roots and providing reference for resistance identification of other crops.
Application Domain
Horticulture methodsPlant tissue culture +1
Technology Topic
ApidaeCrop +8
Examples
- Experimental program(1)
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PUM


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