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Preparation method of rhesus metabolism syndrome model

A technology for metabolic syndrome and rhesus monkeys, applied in animal feed, animal husbandry, etc., can solve the problems of insufficient successful screening ratio, time-consuming and labor-intensive problems, and achieve the effect of strong repeatability and simple administration method

Inactive Publication Date: 2013-10-23
WEST CHINA HOSPITAL SICHUAN UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Screening non-human primates with naturally occurring MetS from large-scale populations is still the main method to obtain such animal models, but this method is very time-consuming and laborious, and the success rate of screening is less than 10%, which is difficult to meet the needs of current research , it is urgent to find a fast and stable modeling method

Method used

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  • Preparation method of rhesus metabolism syndrome model
  • Preparation method of rhesus metabolism syndrome model
  • Preparation method of rhesus metabolism syndrome model

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1 Preparation of the rhesus monkey metabolic syndrome model of the present invention

[0036] 1 Experimental materials and instruments

[0037] Eight 6-month-old rhesus monkeys (half male and half male), free from herpes B virus, simian retrovirus, simian leukemia virus and immunodeficiency virus infection, were purchased from Chengdu Ping An Animal Breeding Base.

[0038] Feed formula:

[0039] Control group: standard monkey feed for monkeys—commercially available monkey pellet feed (the Institute of Zoology, Sichuan Academy of Medical Sciences, the formula is the same as the monkey compound feed stipulated in the "National Standard GB14924.8-2001 of the People's Republic of China").

[0040] Experimental group: high-fat and high-cholesterol feed: 2% (w / w) edible cholesterol (Anhui Tianqi Chemical Technology Co., Ltd.), 15% (w / w) refined lard (commercially available), 5% (w / w) sucrose (commercially available), 78% (w / w) monkey standard monkey chow.

[0041] ...

Embodiment 2

[0105] Example 2 Use the model of the present invention to screen drugs for the treatment of metabolic syndrome

[0106] A, the rhesus monkey metabolic syndrome model established according to the method of Example 1;

[0107] b. administering the drug candidate to an animal model;

[0108] c. Observe the effect of candidate drugs on various indicators of metabolic syndrome, and evaluate potential drugs for treating metabolic syndrome diseases.

[0109] In summary, the modeling method of the present invention induces sugar and lipid metabolism disorders in rhesus monkeys through long-term feeding with high-fat and high-cholesterol diets, and clinical manifestations of metabolic syndrome appear, which is an effective method for establishing a rhesus monkey MetS model.

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Abstract

The invention discloses a preparation method of a rhesus metabolism syndrome model. A rhesus is fed with fat-rich and high-cholesterol feed twice in one day, the feeding capacity of one rhesus is 0.3-0.4 kg / time, the feeding time is not shorter than 6 months, and the feed comprises the following raw materials in parts by weight: 78 parts of standard rhesus feed, 15 parts of animal fat, 5 parts of sugar and 2 parts of cholesterol. With the mode of feeding the fat-rich and high-cholesterol feed for a long term, disorder metabolism of sugar and fat of the rhesus is successfully induced, and the clinical manifestation of the metabolism syndrome is generated, as a result, an effective method for establishing the rhesus MetS model is provided.

Description

technical field [0001] The invention relates to a preparation method of a rhesus monkey metabolic syndrome model. Background technique [0002] Metabolic syndrome (Metabolic Syndrome, MetS) is a group of clinical syndromes with lipid metabolism and glucose metabolism disorders, involving obesity, insulin resistance, blood lipid disorders and hypertension, which can lead to severe cardiovascular and cerebrovascular diseases and Its complications are caused by genetic heterogeneity and environmental factors. The pathogenesis of MetS still has many problems to be studied, and a large number of animal experiments and clinical studies are looking for effective methods of intervention and treatment of MetS. Establishing an ideal MetS animal model is still an urgent problem to be solved in MetS basic research and preclinical experiments. [0003] At present, although there are nearly a hundred MetS animal models of different species and species, it is still very difficult to tran...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K67/02A01K2207/25A01K2227/106A01K2267/0362A23K50/00
Inventor 陈又南程惊秋陆燕蓉刘敬平李新丽杨光张杰廖光能李宏霞王莉袁玉佳何斯荣王成世
Owner WEST CHINA HOSPITAL SICHUAN UNIV