Azalea tissue culture seedling out-bottle rooting method
A tissue culture seedling and exogenous rooting technology, applied in the field of plant tissue culture, can solve the problems such as the lack of a Rhododendron vase exogenous rooting technology, and achieve the effects of improving the level of tissue culture industrialization, saving costs, and saving tissue culture room space.
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Embodiment 1
[0018] Example 1: Rooting methods for native evergreen broad-leaved rhododendron species and varieties of tissue culture seedlings, such as the native species rhododendron versicolor, hybrid species Lord Robert, etc.
[0019] The method proceeds as follows:
[0020] 1) Out-of-bottle rooting time and site selection: In the absence of artificial temperature control, the average temperature is not higher than 20°C, such as choosing October to November for out-of-bottle rooting operations; using greenhouses in the south, the land is leveled, and Equipped with a small shed to place the tray;
[0021] 2) Bottle seedling hardening: After being cultivated in the strong seedling medium, select the bottle seedlings whose plant height has grown to 3-4cm. Move the bottle seedlings from the tissue culture room to the outside of the tissue culture room, and place them under natural light for 2 days; then open the bottle cap twice a day, once in the morning and in the afternoon, for 1 hou...
Embodiment 2
[0025] Example 2: Azalea native species and varieties of tissue culture seedling rooting method outside the bottle, such as varieties Lanying, Dazhusha and so on.
[0026] The method proceeds as follows:
[0027] 1) Out-of-bottle rooting time and site selection: In the absence of artificial temperature control, the average temperature is not higher than 20°C. For example, choose to carry out out-of-bottle rooting operations from December to January; use greenhouses in the south, level the land, and Equipped with a small shed to place the tray;
[0028] 2) Bottle seedling hardening: After being cultivated in the strong seedling medium, select the bottle seedlings whose plant height has grown to 3-4cm; move the bottle seedlings from the tissue culture room to the outside of the tissue culture room, and place them under natural light conditions first. Place it for 3 days; then open the bottle cap twice a day, once in the morning and afternoon, each time for 1 hour, and last fo...
Embodiment 3
[0032] Example 3: Rooting methods for the original species of deciduous Rhododendron and the tissue culture seedlings of the varieties, such as the original species of Azalea, the variety of Red Maple Rhododendron, etc.;
[0033] The transplanting time is selected from February to March, with 105 holes in the hole tray, 70% shading rate in the small arch shed, and thiophanate spray as a broad-spectrum fungicide. Because the temperature rises rapidly in April to May after transplantation, the temperature in the daytime will be affected. When it is higher, the small arch shed should be ventilated at both ends in time; during the period, the broad-spectrum fungicide thiophanate is used to spray and sterilize 2 times, and water 2 times during the rooting period; the remaining measures and methods are the same as in Example 1.
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