Azalea tissue culture seedling out-bottle rooting method
A tissue culture seedling and exogenous rooting technology, applied in the field of plant tissue culture, can solve problems such as the lack of a Rhododendron vase exogenous rooting technology, and achieve the effects of improving the level of tissue culture industrialization, shortening the tissue culture cycle, and saving tissue culture room space.
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Embodiment 1
[0018] Example 1: Rooting methods for native evergreen broad-leaved rhododendron species and varieties of tissue culture seedlings, such as the native species rhododendron versicolor, hybrid species Lord Robert, etc.
[0019] The method proceeds as follows:
[0020] 1) Out-of-bottle rooting time and site selection: In the absence of artificial temperature control, the average temperature is not higher than 20°C, such as choosing October to November for out-of-bottle rooting operations; using greenhouses in the south, the land is leveled, and Equipped with a small shed to place the tray;
[0021] 2) Bottle seedling hardening: After being cultivated in the strong seedling medium, select the bottle seedlings whose plant height has grown to 3-4cm. Move the bottle seedlings from the tissue culture room to the outside of the tissue culture room, and place them under natural light for 2 days; then open the bottle cap twice a day, once in the morning and in the afternoon, for 1 hou...
Embodiment 2
[0025] Example 2: Azalea native species and varieties of tissue culture seedling rooting method outside the bottle, such as varieties Lanying, Dazhusha and so on.
[0026] The method proceeds as follows:
[0027] 1) Out-of-bottle rooting time and site selection: In the absence of artificial temperature control, the average temperature is not higher than 20°C. For example, choose to carry out out-of-bottle rooting operations from December to January; use greenhouses in the south, level the land, and Equipped with a small shed to place the tray;
[0028] 2) Bottle seedling hardening: After being cultivated in the strong seedling medium, select the bottle seedlings whose plant height has grown to 3-4cm; move the bottle seedlings from the tissue culture room to the outside of the tissue culture room, and place them under natural light conditions first. Place it for 3 days; then open the bottle cap twice a day, once in the morning and afternoon, each time for 1 hour, and last fo...
Embodiment 3
[0032] Example 3: Rooting methods for the original species of deciduous Rhododendron and its cultivar tissue culture seedlings, such as the original species of Rhododendron chinensis, the variety of Red Maple Rhododendron, etc.;
[0033] The transplanting time is selected from February to March, with 105 holes in the hole tray, 70% shading rate in the small arch shed, and thiophanate spray as a broad-spectrum fungicide. Because the temperature rises rapidly in 4-5 months after transplantation, the temperature in the daytime will be affected. When it is higher, the small arch shed should be ventilated at both ends in time; during the period, the broad-spectrum fungicide thiophanate is sprayed to sterilize 2 times, and the period of rooting is watered 2 times; the remaining measures and methods are the same as in Example 1.
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