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Azalea tissue culture seedling out-bottle rooting method

A tissue culture seedling and exogenous rooting technology, applied in the field of plant tissue culture, can solve problems such as the lack of a Rhododendron vase exogenous rooting technology, and achieve the effects of improving the level of tissue culture industrialization, shortening the tissue culture cycle, and saving tissue culture room space.

Active Publication Date: 2015-05-27
HANGZHOU BOTANICAL GARDEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wu Lijun et al. reported the technology of out-of-bottle rooting of Photinia fragrans tissue culture (Fujian Forestry Science and Technology, 2007, 34 (4): 4-7), Cheng Shuyun et al. ): 49-50), Huang Zhuozhong reviewed the technology of in vitro rooting in vitro (Guangxi Agricultural Sciences, 2007, 38 (2): 124-126). Except for those mentioned in our research (Zhu Chunyan et al., China Agronomy Bulletin, 2006, 22: 335-337; Garden Science and Technology, 2012, 2: 13-14), there are no domestic reports on the rooting technology of rhododendron vases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Rooting methods for native evergreen broad-leaved rhododendron species and varieties of tissue culture seedlings, such as the native species rhododendron versicolor, hybrid species Lord Robert, etc.

[0019] The method proceeds as follows:

[0020] 1) Out-of-bottle rooting time and site selection: In the absence of artificial temperature control, the average temperature is not higher than 20°C, such as choosing October to November for out-of-bottle rooting operations; using greenhouses in the south, the land is leveled, and Equipped with a small shed to place the tray;

[0021] 2) Bottle seedling hardening: After being cultivated in the strong seedling medium, select the bottle seedlings whose plant height has grown to 3-4cm. Move the bottle seedlings from the tissue culture room to the outside of the tissue culture room, and place them under natural light for 2 days; then open the bottle cap twice a day, once in the morning and in the afternoon, for 1 hou...

Embodiment 2

[0025] Example 2: Azalea native species and varieties of tissue culture seedling rooting method outside the bottle, such as varieties Lanying, Dazhusha and so on.

[0026] The method proceeds as follows:

[0027] 1) Out-of-bottle rooting time and site selection: In the absence of artificial temperature control, the average temperature is not higher than 20°C. For example, choose to carry out out-of-bottle rooting operations from December to January; use greenhouses in the south, level the land, and Equipped with a small shed to place the tray;

[0028] 2) Bottle seedling hardening: After being cultivated in the strong seedling medium, select the bottle seedlings whose plant height has grown to 3-4cm; move the bottle seedlings from the tissue culture room to the outside of the tissue culture room, and place them under natural light conditions first. Place it for 3 days; then open the bottle cap twice a day, once in the morning and afternoon, each time for 1 hour, and last fo...

Embodiment 3

[0032] Example 3: Rooting methods for the original species of deciduous Rhododendron and its cultivar tissue culture seedlings, such as the original species of Rhododendron chinensis, the variety of Red Maple Rhododendron, etc.;

[0033] The transplanting time is selected from February to March, with 105 holes in the hole tray, 70% shading rate in the small arch shed, and thiophanate spray as a broad-spectrum fungicide. Because the temperature rises rapidly in 4-5 months after transplantation, the temperature in the daytime will be affected. When it is higher, the small arch shed should be ventilated at both ends in time; during the period, the broad-spectrum fungicide thiophanate is sprayed to sterilize 2 times, and the period of rooting is watered 2 times; the remaining measures and methods are the same as in Example 1.

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Abstract

The invention relates to an azalea tissue culture seedling out-bottle rooting method which includes the steps of 1) selecting out-bottle rooting time and site; 2) hardening bottle seedlings; 3) preparing substrate; 4) cutting tissue culture seedlings; 5) seedling hardening after cutting and light and water management. An effective azalea tissue culture seedling out-bottle rooting generic technology is provided, the bottleneck that some azalea subgenus tissue culture seedlings are difficult in rooting is solved, and the method is applicable to most azalea subgenus and varieties. By the method, an azalea tissue culture rapid propagation system is completed, tissue culture in-bottle rooting is unnecessary, tissue culture space is saved by more than 50%, cost of tissue culture seedlings is saved by more than 30%, tissue cycle is shortened greatly, tissue culture seedling out-bottle rooting rate can be reach to 80-95%, and great significance is provided for improving tissue culture industrialization level.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for rooting rhododendron tissue cultured seedlings outside a bottle. Background technique [0002] Rhododendron ( Rhododendron ) is a famous ornamental flower in the world. There are nearly a thousand native species of Rhododendron in the world, and my country accounts for about 60%. It is the birthplace and distribution center of Rhododendron. There are more than 10,000 varieties obtained through hybridization in the world, and the germplasm resources are very rich. There are many kinds of rhododendrons, and the flowers are large and colorful. They have high ornamental value and are known as the beauty of flowers. Rhododendron resources in my country are spread all over the country, especially in Yunnan, Guizhou, Sichuan, and Tibet in the southwest. In the utilization of gardens, my country has formed azalea industrial bases represented by Fujian, Zhejia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00A01H4/00
Inventor 朱春艳顾宏辉盛小光朱丹华
Owner HANGZHOU BOTANICAL GARDEN
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