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Methylated fucus evanescens oligose or methylated sulfated fucus evanescens oligose or salt thereof as well as preparation method and application thereof

A technology of methylated fucoidal oligosaccharides and esterified fucoidal oligosaccharides, applied in the field of methylated fucoidal oligosaccharides or methylated sulfated fucoidal oligosaccharides or their salts and their preparation and application

Active Publication Date: 2013-10-30
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzyme is an important means for structural analysis, but there is no commercially available enzyme to decompose fucoidan sulfate, and the structure of fucoidan sulfate varies with different sources, so partial desulfation of it is important for structural analysis and Great convenience in terms of separation

Method used

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  • Methylated fucus evanescens oligose or methylated sulfated fucus evanescens oligose or salt thereof as well as preparation method and application thereof
  • Methylated fucus evanescens oligose or methylated sulfated fucus evanescens oligose or salt thereof as well as preparation method and application thereof
  • Methylated fucus evanescens oligose or methylated sulfated fucus evanescens oligose or salt thereof as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Preparation, purification and structural analysis of methylated fucoidal oligosaccharides or their salts and methylated sulfated fucoidal oligosaccharides or their salts.

[0049] (1) Preparation of methylated fucooligosaccharide or its salt and methylated sulfated fucooligosaccharide or its salt mixture

[0050] The preparation method of methylated sulfated fucoidan oligosaccharides, which uses laminaria fucoidan sulfate as raw material, can be operated according to the following steps, the fucoidan sulfate is dissolved in a DMSO / methanol solution with a volume ratio of 10%, and the brown algae The mass concentration of polysaccharide sulfate in DMSO / methanol solution is 1.5%, the reaction temperature is 90°C, and the reaction time is 10h, and the obtained degradation mixture is obtained; Molecular substances, the retentate was concentrated and freeze-dried to obtain the oligosaccharide mixture A. The resulting oligosaccharide mixture A was analyzed for mole...

Embodiment 2

[0106] Example 2 Preparation, purification and structural analysis of methylated fucoidal oligosaccharides or their salts and methylated sulfated fucoidal oligosaccharides or their salts.

[0107] (1) Preparation of methylated fucooligosaccharide or its salt and methylated sulfated fucooligosaccharide or its salt mixture

[0108] The preparation method of methylated sulfated fucoidan oligosaccharide, which uses fucoidan sulfate as raw material, can be operated according to the following steps: 1) Weigh fucoidan sulfate and dissolve it in a volume ratio of 10% DMSO / methanol solution, The mass concentration of fucoidan sulfate is 0.5%, the reaction temperature is 80° C., and the reaction time is 5 hours; 2) After the reaction is completed, tap water is dialyzed for 2-3 days, and distilled water is dialyzed for 1-2 days. Freeze dried. Thereby obtaining the oligosaccharide mixture, with Figure 17 Its ESI-MS figure.

[0109] (2) Refining and structural analysis

[0110] Purifi...

Embodiment 3

[0111] Example 3 Preparation, purification and structural analysis of methylated fucoidal oligosaccharides or their salts and methylated sulfated fucoidal oligosaccharides or their salts.

[0112] (1) Preparation of methylated fucooligosaccharide or its salt and methylated sulfated fucooligosaccharide or its salt mixture

[0113] The preparation method of methylated sulfated fucoidan oligosaccharide, which uses fucoidan sulfate as raw material, can be operated according to the following steps: 1) Weigh fucoidan sulfate and dissolve it in a volume ratio of 10% DMSO / methanol solution, The mass concentration of fucoidan sulfate is 5%, the reaction temperature is 80° C., and the reaction time is 5 hours; 2) After the reaction is completed, tap water is dialyzed for 2-3 days, and distilled water is dialyzed for 1-2 days. Freeze dried. Thereby obtaining the oligosaccharide mixture, with Figure 18 Its ESI-MS figure.

[0114] (2) Refining and structural analysis

[0115] Purifica...

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Abstract

The invention relates to serial methylated fucus evanescens oligose or methylated sulfated fucus evanescens oligose or a salt thereof as well as a preparation method thereof and application thereof in preparing an anti-parkison medicament. The preparation method comprises the following steps of: preparing oligose by carrying out desulphidation esterification onto fucoidan polysaccharide sulfate; purifying and separating by column chromatography to obtain serial high-purity methylated fucus evanescens oligose and methylated sulfated fucus evanescens oligose. The methylated fucus evanescens oligose and methylated sulfated fucus evanescens oligose have a nerve protection effect for nerve cell injuries induced by neurotoxin, and can be used for medicaments and health-care products for preventing and treating neurodegenerative diseases.

Description

technical field [0001] The present invention relates to methylated fucoid oligosaccharide or methylated sulfated fuco oligosaccharide or salt thereof and preparation method and application thereof. Background technique [0002] Fucoidan sulfate is a sulfated polysaccharide from brown algae, which has various functions such as anticoagulant, anti-inflammatory, anti-tumor, anti-virus, and anti-ulcer. However, due to the different sources of brown algae (including breeding places, picking time, species, etc.), the obtained structures are also different. The known ones are sulfated fucoidan, sulfated fucoglucuronomannan, sulfuric acid Fucogalactan, etc. [0003] For the development of sulfated polysaccharides as pharmaceuticals, it is necessary to determine their structure. Enzyme is an important means for structural analysis, but there is no commercially available enzyme to decompose fucoidan sulfate, and the structure of fucoidan sulfate varies with different sources, so par...

Claims

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Application Information

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IPC IPC(8): C08B37/08C07H15/04C07H1/00A61K31/737A61K31/715A61K31/7028A61P25/16A61P25/28A23L1/09A23L33/125
Inventor 张全斌金维华符大勇王晶张虹牛锡珍
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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