Tissue-culture rapid propagation method of primula oreodoxa franch
A tissue culture rapid propagation and culture medium technology, applied in the field of plant tissue culture, can solve the problems of no relevant research and reports, failure to meet production needs, and restrictions on large-scale planting, so as to shorten the seedling breeding cycle, increase the number of reproduction, and achieve consistency strong effect
Active Publication Date: 2015-02-18
四川省自然资源科学研究院
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Problems solved by technology
Under natural conditions, the seeds of this flower are small and shriveled, the seed reproduction rate is low, the growth cycle is long, and the emergence of seedlings is irregular, which limits the large-scale planting of this flower and cannot meet the production needs.
Tissue culture is an important way of rapid propagation of fine plant varieties. There have been reports on tissue culture of Primula euphratica, Primula wrinkled leaves, Primula takaspica, Primula small, and Primula northern Yunnan in the same genus. There is no relevant research and report on the tissue culture of Primula japonica at home and abroad, so it is necessary to carry out research on the tissue culture of Primula japonica and establish a set of special rapid propagation technology system
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[0031] A kind of Primula japonica tissue culture rapid propagation method comprises the following steps:
[0032] Choose the middle and lower part 1 of the blade of Primula Primula to be the explant (combined with figure 1 and figure 2 As shown, in this embodiment, the middle and lower part of the blade is 2 / 3 of the blade along the direction from the tip 2 to the petiole 3, and the remaining 1 / 3 of the blade is the middle and lower part of the blade 1). Ultra-clean workbench, sterilize the surface with 75% alcohol for 30s, rinse with sterile water for 2-3 times, then disinfect with 0.1% HgCl2 solution for 5-8min, take it out and wash it repeatedly with sterile water for 3-4 times, aseptic Blot the water with filter paper, cut off the injured tissue around with scissors, then cut the leaves into small pieces of 0.5cmx0.5cm, and inoculate them in adventitious bud induction medium MS+3.0mg / L6-BA+0.5mg / LNAA with the leaf side down, sucrose 35g / L, agar 5 g / L, and pH 5.8-6.0, th...
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The invention discloses a tissue-culture rapid propagation method of primula oreodoxa franch. The method comprises the steps of selection and sterilization of explants, induction of adventitious buds, subculture propagation cultivation, cultivation for roots and strong seedlings and transplantation of test-tube seedlings. According to the invention, the propagation coefficient of primula oreodoxa franch is improved, the growth cycle of primula oreodoxa franch is shortened, high quality seedlings can be obtained in a short time, and the germplasm preservation as well as application and promotion of primula oreodoxa franch is technically guaranteed.
Description
technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of Primula oreodoxa Franch. Background technique [0002] Primula oreodoxa Franch is a perennial evergreen herb of the genus Primula in the family Primulaceae. It was discovered in 1886, and its type origin is Mount Emei in Sichuan, my country. It is distributed in the northwest of Sichuan (Mabian, Emei, Tianquan, Baoxing, Guanxian) and other places. It is a unique species in Sichuan. Under natural conditions, it blooms from April to May. The scape is 5-14 cm high, erect, covered with coarse hairs; the umbel has 3-10 small flowers, and the flowers open successively from top to bottom, purple-red, very gorgeous, with high Its ornamental value can be used as potted plants, ground covers, and flowers for flower beds. Under natural conditions, the seeds of this flower are small and shriveled, the seed reproduction rate ...
Claims
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IPC IPC(8): A01H4/00
Inventor 余道平李策宏向丽谷海燕张国珍李小杰
Owner 四川省自然资源科学研究院