Method for quickly repairing injured anaerobic ammonium oxidation bacterium groups

A technology of anaerobic ammonium oxidizing bacteria and flora, applied in chemical instruments and methods, bacteria, stimulating growth by adding compounds, etc., can solve problems such as gaps in research and application fields, to be explained and disclosed

Active Publication Date: 2013-12-04
PEKING UNIV
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Problems solved by technology

Although there are more and more reports on the regulation of bacterial biochemical behavior by homoserine lactone signal molecules, there is almost no research and application field on the regulation mechanism

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  • Method for quickly repairing injured anaerobic ammonium oxidation bacterium groups
  • Method for quickly repairing injured anaerobic ammonium oxidation bacterium groups
  • Method for quickly repairing injured anaerobic ammonium oxidation bacterium groups

Examples

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Example Embodiment

[0019] The following will further illustrate the present invention in combination with specific embodiments, but not as a limitation on the scope of implementation of the present invention.

[0020] The example of repairing damaged anammox bacterial flora of the present invention: in the samples used, anammox bacteria account for 50-60% of the total composition of the bacterial flora, and more than half of the bacterial cells have been damaged or died . Chemical analysis is used to determine ammonia oxidation activity and bacterial population proliferation, and the proportion of dead bacteria and live bacteria to the entire bacterial population is analyzed by the identification of dead bacteria.

[0021] Divide the damaged anammox bacteria group into 4 equal groups. The 4 groups were treated as follows: 1 group did not add signal factor; 2 groups added C6-HSL (30mg / L); 3 groups added C8-HSL (30mg / L); 4 groups added C12-HSL (30mg / L). Before the test, the dry weight of each group o...

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Abstract

The invention relates to a novel method for quickly repairing injured anaerobic ammonium oxidation bacterium groups. The novel method is characterized in that homoserine lactone signal molecules C6-HSL (acyl-homoserine lactone) are added into the injured anaerobic ammonium oxidation bacterium groups to improve the activity of anaerobic ammonium oxidation bacteria, increase the reproduction rate of the anaerobic ammonium oxidation bacteria and relieve inactivation and death causing effects of external inhibitors on the anaerobic ammonia oxidation bacteria; owing to added homoserine lactone signal molecules C8-HSL, the reproduction rate of the anaerobic ammonium oxidation bacterium groups can be increased, and repair for the injured anaerobic ammonium oxidation bacterium groups can be accelerated. The novel method has the advantages that the novel method is simple and feasible, effects are obvious, the application range is wide, the bottleneck problem of inactivation or death of anaerobic ammonium oxidation bacteria due to the fact that the anaerobic ammonium oxidation bacteria can be easily inhibited during actual application is solved, and the novel method can be used for implementing relevant anaerobic ammonium oxidation processes and efficiently biologically denitrifying sewage.

Description

Technical field: [0001] The invention relates to the field of biological technology, in particular to the regulation and control of homoserine lactone signal molecules on the behavior of anaerobic ammox bacteria group and its application in the field of sewage biological treatment. Background technique: [0002] Anaerobic ammonium oxidation refers to the conversion of ammonia nitrogen and nitrite nitrogen into nitrogen under anaerobic conditions. It is a new generation of sewage treatment technology. A famous Dutch scholar published an article in Science and pointed out that if the combination of anaerobic ammonium oxidation technology and methane production technology replaces traditional biological denitrification technology for sewage treatment, it will change water treatment from energy consumption to production capacity, and generate Huge economic and social benefits [2]. Although more and more pilot-scale and production-scale anammox reactors have been built in Europe...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/20C02F3/34C02F101/16
Inventor 刘思彤胡松禾倪晋仁张作涛
Owner PEKING UNIV
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