Protein fingerprint atlas-spectrum model of acidovorax avenae subsp. citrulli and applications thereof

A oat acid phage and fingerprint technology, applied in the field of microbial detection, can solve the problem of no protein fingerprint model detection method, etc., and achieve the effect of strong specificity

Inactive Publication Date: 2013-12-04
SHENZHEN AUDAQUE DATA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no protein fingerprint model for detection or identification of A. avena

Method used

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  • Protein fingerprint atlas-spectrum model of acidovorax avenae subsp. citrulli and applications thereof
  • Protein fingerprint atlas-spectrum model of acidovorax avenae subsp. citrulli and applications thereof
  • Protein fingerprint atlas-spectrum model of acidovorax avenae subsp. citrulli and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] 1. Materials

[0032] 1. Tested strains

[0033] In this example, 22 strains of A. avenatum subsp. watermelon and 17 strains of A. avenatum subsp. watermelon similar species were used for testing. Among them, 22 strains of A. avenatum subspecies watermelon include the numbers 470144 (ZZ-1), 470141 (SY-1), 470142 (SY-3), 470150 (LS-2) provided by the Seed Pathology Laboratory of China Agricultural University. ), 470138 (LS-3), 470140 (LS-10), 470131 (XJ-1), 470133 (XJ-4), 470139 (LS-5), 470136 (XJ-6), 470152 (BJ-A) The 10 strains provided by the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 470145 (pslb-25) and No. 470149 (FC512), 470126 (FC376), 470125 (FC374) provided by USDA / ARS / FDWSRU , 470148 (FC455), 470123 (FC248), 470122 (FC247T, ATCC29625), 470121 (FC183), 470127 (FC380), 470130 (FC520), 470151 (pslb91) 10 strains. The 17 other tested strains include 4 Acidovorax avenae subsp.avenae strains provided by USDA / ARS / FDWSRU with numbe...

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Abstract

The invention disclose a protein fingerprint atlas-spectrum model of acidovorax avenae subsp. citrulli, the model is drawn according to the mass-to-charge ratios of acidovorax avenae subsp. citrulli protein and wave crest strength coefficients thereof, wherein the mass-to-charge ratios comprises: 3171.4, 3735.0, 4963.5, 5139.0, 5866.4, 6554.1, 7466.1, 7948.0, 8384.5, 9304.2, 9504.3, 9920.0, 10272.8, 10840.7, 12530.5, and 12896.5. The invention also discloses a matrix-assisted laser desorption/ionization flying time mass spectrum detection method based on the model. The method can qualitatively detect whether a sample contains acidovorax avenae subsp. citrulli. The model has a function of specific recognition, is capable of being applied to commercial detection of acidovorax avenae subsp. citrulli, and is especially suitable for rapid and high throughout detection for departments such as port inspection and quarantine.

Description

technical field [0001] The invention relates to the field of microbial detection, in particular to a protein fingerprint model of A. avenae subsp. watermelon and the application of the model in the detection and identification of A. avenae subsp. watermelon. Background technique [0002] Bacterial fruit spot of watermelon is a worldwide disease that seriously damages Cucurbitaceae crops, especially watermelon and muskmelon. The pathogen of the disease was first named Pseudomonas pseudoalcaligens subsp.citrulli by Schaad et al. In 1992, Willems et al. renamed it Acidovorax avenae subsp. citrulli (Aac.) based on the hybridization results of rRNA-RNA and DNA-DNA molecules. At present, the disease occurs every year in more than 10 states in the southeastern United States. Although the area of ​​occurrence is less than 5% in some years, the loss of diseased fields can be as high as 90% to 100%. Because the pathogen can be carried by seeds, it has caused a huge impact and threat...

Claims

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Application Information

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IPC IPC(8): G01N27/64
Inventor 冯建军章桂明李健强郑耘李一农娄定风
Owner SHENZHEN AUDAQUE DATA TECH
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