A New Method of Sexual Hybridization of Rice

Abstract

The invention discloses a novel male-killing method for sexual hybridization of rice. During the sexual hybridization of the rice, from the afternoon of the day before to the very day before blossom, non-bloomed glumous flowers of a hybridization female parent are cut off by 1 / 4-3 / 5 in a manner of breaking the anther, not damaging the stigma and immediately spraying a vinegar liquid with the concentration of 0.3-0.5 percent, so that the death of pollens is accelerated, and a male-killing effect can be achieved; before pollination, rice ears which are subjected to glumous flower cutting and anther breakage and are sprayed with the vinegar liquid are hit for several times with fingers to remove the anther in the glumous flowers out of shells of the glumous flowers; then the rice ears are covered by bags; when a male parent blooms, male-parent pollination hybridization is executed. By the adoption of the glumous flower cutting, anther breakage and vinegar liquid spraying method, the sexual hybridization of the rice (or wild rice) can be greatly executed, and therefore, the sexual hybridization efficiency is improved; the glumous flower cutting, anther breakage and vinegar liquid spraying rate can be 100 percent; the method is convenient, quick and good in effect; compared with a conventional sexual hybridization method, the method has the advantages that a large number of hybridized seeds can be obtained, the demand for a large number of hybridization groups can be met, and the sexual hybridization efficiency is greatly improved.

Description

technical field [0001] The invention relates to the category of rice sexual hybridization methods, in particular to a rice hybridization method in which glumes are broken, herbs are sprayed with vinegar liquid to kill males. Background technique [0002] my country is not only a big country of grain production, but also a big country of food consumption. Rice is the largest food crop. In rice improvement and breeding, the most basic method of rice new variety breeding is hybrid breeding. In this process, rice varieties are sexually hybridized, and most of the female parents are killed by traditional methods. "Warm soup" detasseling and pruning artificial detasseling is inconvenient, time-consuming and laborious, and the efficiency of sexual hybridization is low. Especially in the research of biotechnology breeding, a large number of groups are required, and the existing sexual hybridization methods are difficult to achieve, such as "Global molecular breeding" requires the c...

AI Technical Summary

Problems solved by technology

Rice is the largest food crop. In rice improvement and breeding, the most basic method of rice new variety breeding is hybrid breeding. In this process, rice varieties are sexually hybridized, and most of the female parents are killed by traditional methods. "Warm soup" detasseling and pruning artificial detasseling is inconvenient, time-consuming and laborious, and the efficiency of sexual hybridization is low. Especially in the research of biotechnology breeding, a large number of groups are required, and the existing sexual hybridization methods are difficult to achieve, such as "Global molecular breeding" requires the construction of a large number of near-isogenic lines, using 118 donor parents and 2 donor parents. Each donor is hybridized and backcrossed with the donor parent. In the backcross generation, the donor parent It is necessary to randomly select 25 individual plants for backcrossing with each backcrossing group, and each backcrossing generation needs to do 5,000 sexual crosses in one season, which cannot be achieved by conventional sexual crossing methods

The reason is that the artificial detasseling process is time-consuming and labor-intensive, and the efficiency of sexual hybridization is very low

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