Method for establishing analysis immune library spectra based on random primer barcode technology
A technology of random primers and immune library, applied in the field of individualized diagnosis and treatment, can solve the problems of large sequencing error and amplification skew of low copy number sequences
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[0026] Preferred embodiments of the present invention will be described in detail below. The experimental method that does not indicate specific condition in the preferred embodiment, generally according to conventional conditions, such as Molecular Cloning Experiment Guideline (third edition, J. Sambrook etc., translated by Huang Peitang, etc., Science Press, 2002) described conditions, or as recommended by the manufacturer.
[0027] A sample selection of patients with chronic hepatitis B
[0028] (1) Select a patient with mild chronic hepatitis B: mild and few symptoms, such as mild fatigue, mild abdominal distension, mild liver discomfort, etc., and liver function damage. Transaminase is 3 times (normal value is 40 units), bilirubin is about 2 times normal value (normal value is 17.1 micromol / L), albumin is greater than or equal to 35 g / L (normal value is 35-55 g / L) Lift).
[0029] (2) Select one person with moderate chronic hepatitis B: the symptoms and signs and liver ...
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