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Extraction and thin-layer chromatographic scanning detection method for octacosanol from Antarctic krill

A technology of octadecanol and Antarctic krill, which is applied in the directions of measuring devices, analytical materials, instruments, etc., can solve the problems of high cost and long time, and achieve the effect of important application value

Inactive Publication Date: 2015-06-17
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gas and liquid chromatography detection methods are expensive and time-consuming

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh 60 g of dried Antarctic krill, add 360 ml of n-hexane, extract 3 times, filter the combined extracts, evaporate the n-hexane to obtain 3 g of Antarctic krill shrimp oil. Add 120ml of 10% NaOH ethanol solution to the shrimp oil, heat in a water bath at 80°C, and reflux for 2 hours; cool down to 50°C, add 120ml of petroleum ether, and extract at 70°C for 40 minutes; then add 120ml of water, stir thoroughly for 20 minutes, and reflux at 50°C Stand still and separate layers; take the petroleum ether solution in the upper layer, evaporate the petroleum ether and dissolve it with chloroform to obtain 8ml of sample solution.

[0024] Accurately weigh 1 mg of octacosanol standard substance (Sigma Company), add chloroform to dilute to 4 ml, and prepare a standard solution with a concentration of 0.25 mg / ml. Precisely draw 1.5, 2, 2.5, 3, 4.5ul octacosanol standard solution and 1.5ul sample solution on the same high-efficiency thin-layer silica gel plate GF 254 (10cm×10cm)...

Embodiment 2

[0026] The difference from Example 1 is that the developing agent is 15ml, the developing agent is petroleum ether: ethyl acetate: toluene=5:1.5:0.1, the dyeing agent is 80ml, and the staining liquid formula is water: bromothymol blue: sodium hydroxide =80ml: 0.08g: 0.6g, other steps are the same as in Example 1, according to the linear regression equation in Example 1, the average content of octacosanol in 8ml of sample solution (ie 60g of dried Antarctic krill) is 3.181mg.

Embodiment 3

[0028] The difference from Example 1 is that the developing agent is 12ml, the developing agent is petroleum ether: ethyl acetate: toluene=3:0.5:0.05, the dyeing agent is 110ml, and the staining liquid formula is water: bromothymol blue: sodium hydroxide =110ml: 0.12g: 0.8g, other steps are the same as in Example 1, according to the linear regression equation in Example 1, the average content of octacosanol in 8ml of sample solution (ie 60g of dried Antarctic krill) is 3.183mg.

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PUM

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Abstract

The invention discloses an extraction and thin-layer chromatographic scanning detection method for octacosanol from Antarctic krill which specifically comprises the following steps: extracting Antarctic krill oil, performing heating reflux on the Antarctic krill oil through an ethanol solution of NaOH, extracting with petroleum ether, and removing the petroleum ether through evaporation, so as to obtain the octacosanoll; preparing a sample solution and a standard solution respectively, dripping the solutions onto a silica-gel plate GF 254 of high-performance thin-layer chromatography respectively, developing, dyeing, performing thin-layer chromatographic scanning to obtain the Rf value and the peak area integral value of octacosanol, calculating the relationship between the quality and the peak area integral value of the standard solution, and calculating the quality of the octacosanol in the sample solution according to the obtained relationship. The extraction and thin-layer chromatographic scanning detection method disclosed by the invention can be used for extracting and detecting octacosanol in Antarctic krill, more importantly can be used for detecting the content of octacosanol in any octacosanol-contained raw materials, products and byproducts, and has a wide and important application value in octacosanol development.

Description

technical field [0001] The invention relates to an extraction and thin-layer chromatography scanning detection method of Antarctic krill octacosanol. Background technique [0002] Octacosanol is a naturally occurring monohydric higher alcohol, which is widely found in the lipids of roots, stems, leaves, shells and seeds of plants, and in waxes secreted by insects (such as white wax, shellac wax, Beeswax, etc.), widely distributed in animal skin, viscera, animal lipids (such as wool wax, spermaceti, fish egg lipids and marine crustaceans, etc.). [0003] In 1949, Dr. Cureton of the University of Illinois in the United States used natural octacosanol and synthetic octacosanol to conduct 42 experiments on animals and 894 subjects, and made significant progress. Cureton's research results show that octacosanol has the following effects on the human body: ① Improve endurance and energy; ② Improve responsiveness; ③ Improve stress ability; ④ Promote hormone action and reduce muscl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
Inventor 高文芳刘代成
Owner SHANDONG NORMAL UNIV