Liquid chip kit for detection of lung cancer

A liquid phase chip and kit technology, applied in the field of medicine and biology, can solve the problems of incomplete clarity and complexity of the cytological functions of Axl and Gas6 proteins, and achieve the effects of high-throughput detection, high diagnostic accuracy and low cost.

Inactive Publication Date: 2013-12-11
ZHEJIANG ACAD OF MEDICAL SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The cellular functions of Axl and Gas6 proteins ...

Method used

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  • Liquid chip kit for detection of lung cancer
  • Liquid chip kit for detection of lung cancer
  • Liquid chip kit for detection of lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The preparation of embodiment 1 liquid phase chip

[0042] 1. Reagents and solutions

[0043] (1) pH6.2, 0.1M NaH 2 PO 4 : Weigh 3.5814g NaH 2 PO 4 in 90mL ddH 2 In O, adjust the pH to 6.2 with NaOH, dilute to 100mL, and filter with a 0.22um filter;

[0044] (2) pH5.0, 0.05M MES: weigh 0.976g MES in 90mL ddH 2 In O, adjust the pH to 5.0 with NaOH, dilute to 100mL, and filter with a 0.22um filter;

[0045] (3) pH6.0, 0.1M MES: weigh 1.952g MES in 90mL ddH 2 In O, adjust the pH to 6.0 with NaOH, dilute to 100mL, and filter with a 0.22um filter;

[0046] (4) pH4.5, 0.1M MES: weigh 1.952g MES in 90mL ddH 2 In O, adjust the pH to 4.5 with NaOH, dilute to 100mL, and filter with a 0.22um filter;

[0047] (5) PBS: NaCl, 137mM; KCl, 2.7mM; NaCl 2 HPO 4 , 8.1mM; KH 2 PO 4 , 1.5mM; pH7.2-7.4; 0.22um membrane filtration;

[0048] (6) 1%PBSB: 0.1%PBS containing 0.02%Proclin300, 0.22um membrane filtration;

[0049] (7) 0.1%PBST: 0.1%PBS containing 0.1%tween-20, 0.22um...

Embodiment 2

[0089] Embodiment 2 The liquid phase chip kit of the present invention is used for detecting lung cancer test

[0090] 1. Detection method

[0091] (1) Take out all reagents before use, and let them balance to room temperature;

[0092] (2) Take out the serum of patients (patients with early lung cancer) and healthy people at -80°C, place them on ice to melt, vortex and mix well, and take a V-type 96-well hemagglutination plate (96-well plate) to dilute the serum 20 times , mix well with the tip of the pipette up and down, try not to generate air bubbles;

[0093] (3) Prepare CRP, Prolactin, CEA, NSE, CK19, Axl and ADAM8 standard protein (CRP, product number is DY2648, R&D system; Prolactin, product number is DY682, R&D system; CEA, product number is 73 / 601, NIBSC ; NSE, the product number is DENL20, R&D system; CK19, the product number is 30R-AC04, Fitzgerald Industries International; Axl, the product number is DY154, R&D system; Adam8, the product number is DY1031, R&D sys...

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Abstract

The invention discloses a liquid chip kit for detection of a lung cancer. The liquid chip kit comprises coating microspheres, a biotin-labeled detection antibody, streptavidin-phycoerythrin, a reaction buffer solution and a dilution buffer solution, wherein the coating microspheres comprise at least two selected from the group consisting of a microsphere coating a CRP capture antibody, a microsphere coating a Prolactin capture antibody, a microsphere coating a CEA capture antibody, a microsphere coating an NSE capture antibody, a microsphere coating a CK19 capture antibody, a microsphere coating an Axl capture antibody and a microsphere coating an ADAM8 capture antibody, the microspheres coating different capture antibodies have different color codes, and detection antibodies correspond to the capture antibodies. The liquid chip kit provided by the invention has the advantages of high sensitivity and accuracy, rapid detection, good stability and low cost.

Description

technical field [0001] The invention relates to the field of medicine and biology, in particular to a liquid phase chip kit for detecting lung cancer. Background technique [0002] In recent years, the incidence of cancer in my country has shown an obvious upward trend, accounting for more than 20% of the causes of death, ranking first among all causes of death. The World Health Organization predicts that by 2020, there will be 20 million new cancer cases and 12 million deaths, most of which will occur in developing countries. If no effective prevention and control measures are taken, it is estimated that by 2020, the total number of new cancers and cancer deaths in my country will reach about 3 million, and the total number of patients will reach 6.6 million. [0003] Lung cancer is currently the malignant tumor with the highest morbidity and mortality worldwide. In the past 20 years, due to vigorous promotion of smoking cessation, the incidence of male lung cancer in Eur...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/574G01N33/573
Inventor 王孝举马胜林吴国君王闻哲夏冰陈雪琴
Owner ZHEJIANG ACAD OF MEDICAL SCI
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