Method of operation of fermentation of gaseous substrate comprising hydrogen
A gaseous substrate, hydrogen technology, applied in biochemical equipment and methods, fermentation, gas production bioreactors, etc., can solve problems such as low cell density
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[0050] As an embodiment of the present disclosure, the substrate comprising CO comprises hydrogen.
[0051] As an embodiment of the present disclosure, the method comprises adding a nutrient medium to the bioreactor.
[0052] figure 1 A method for producing a chemical species such as an alcohol product mixture by microbial fermentation from a gaseous substrate comprising carbon monoxide (CO), such as syngas, is shown, wherein the method comprises a bioreactor (100) containing the Fermentation broth of microbial cells and fermentation medium. A gaseous stream ( 101 ) comprising a CO-containing gaseous substrate may be fed into the bioreactor together with a fermentation medium stream ( 102 ). A fermentation broth stream (110) comprising the microbial cells and the chemical species product may be removed from the bioreactor. A fermentor vent gas stream (120), comprising an unused portion of said gaseous stream comprising gaseous substrate, is discharged from the bioreactor. ...
Embodiment 1
[0111] Example 1: Clostridium ljungdahalii PETC: Increased Bacteria Density in Reactor by Maintaining Percent Hydrogen Uptake at 4.5% of Total Gas Flowing into Reactor
[0112] The New Brunswick bioflow I reactor containing the fermentation medium initially contained 0.34 g / L of an actively growing C. ljungdahlii PETC strain. At the start of the experiment, the stirring rate of the reactor was set to 500 rpm. This stirring rate was maintained throughout the experiment. The temperature in the bioreactor was maintained in the range of about 38 to about 39°C throughout the experiment. Take samples of syngas feed to the bioreactor, vent gas from the bioreactor, and fermentation broth in the bioreactor at regular intervals (e.g., every 1 hour) and analyze the consumption of various gas components Or production, the concentration of acetic acid in the medium, the concentration of ethanol in the medium, and the optical density (cell density) of the culture.
[0113] H 2 The molar...
Embodiment 2
[0124] Example 2: Clostridium ljungdahlii C-01: Increased microbial density in the reactor by maintaining the percent hydrogen uptake at approximately 3% of the total gas flowing into the reactor
[0125] A New Brunswick Bioflow I reactor containing about 1.5 liters (eg, in the range of about 1.45 liters to about 1.6 liters) of fermentation medium initially contained about 0.38 g / L of actively growing C. ljungdahlii strain C-01. Before the start of the experiment, the stirring rate in the bioreactor was set to 600 rpm. This stirring rate was maintained throughout the experiment. The temperature in the bioreactor was maintained in the range of about 36 to about 37.5°C throughout the experiment. Take samples of syngas feed to the bioreactor, vent gas from the bioreactor, and fermentation broth in the bioreactor at regular intervals (e.g., every 1 hour) and analyze the consumption of various gas components Or production, the concentration of acetic acid in the culture medium, t...
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