Method for extracting hinokiflavone from Cacumen Biotae

A technology of cypress biflavonoids and cypress leaves, applied in the biological field, can solve the problems of large amount of reagents, complicated purification process, and difficulty in industrial production
CN103450136AInactive Publication Date: 2013-12-18NANJING BIAOKE BIO TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING BIAOKE BIO TECH
Publication Date
2013-12-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for extracting hinokiflavone from Cacumen Biotae. The method comprises the following steps: 1) pulverizing Cacumen Biotae, adding 5-10 times of saturated lime water for immersion and extraction for 2-3 times, ultrafiltering the extracting solution through an ultrafiltration membrane, and concentrating the filtrate with a nanofiltration membrane to obtain a concentrated solution; 2) regulating the pH value of the concentrated solution to 3-5, adding into a macroporous resin column for adsorption, carrying out gradient elution with an ethanol solution, concentrating the eluate, regulating the pH value to 3-5, and crystallizing; and 3) dissolving the filtered crystal in a 50-80% methanol solution, concentrating until the methanol concentration is 10-30%, adding into a macroporous resin for adsorption, carrying out gradient elution with an ethanol solution, concentrating the eluate for crystallization, recrystallizing the crystal with a 70% ethanol solution, and drying. The method for producing hinokiflavone has the advantages of simple technological operation, low energy consumption and small pollution, and can easily implement industrial production.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting cypress biflavone from leaves of oriental cypress. Background technique

[0002] Cypress biflavonoids are flavonoids, CAS number 19202-36-9, melting point 353-355°, molecular weight C30H18O10, molecular weight 538.47. Molecular formula:

[0003]

[0004] Modern studies have found that cypress biflavone has anti-inflammatory, antibacterial and anti-tumor effects.

[0005] Through literature search, the preparation method of cypress biflavone mostly adopts chromatography, such as the patent "a preparation method of cypress biflavone", the method disclosed in this patent adopts polyamide chromatographic column separation. This type of method requires a large amount of reagents and a complicated purification process, making it difficult to realize industrial production. Contents of the invention

[0006] The technical problem to be solved by th...

Claims

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