Saline-alkali tolerant trichoderma harzianum and application thereof

A technology of tolerance and bacterial strains, applied in the direction of application, chemicals for biological control, biocides, etc., can solve the problems of destroying agricultural ecosystems, environmental pollution, etc., achieve the goal of preventing and controlling fungal diseases and maintaining the balance of agricultural ecosystems Effect

Active Publication Date: 2013-12-18
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, chemical agents are mostly used for prevention and control in production, but long-term and large-scale use of pest

Method used

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  • Saline-alkali tolerant trichoderma harzianum and application thereof
  • Saline-alkali tolerant trichoderma harzianum and application thereof
  • Saline-alkali tolerant trichoderma harzianum and application thereof

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Embodiment 1

[0059] Embodiment 1, characteristics and identification of Trichoderma harzianum 29HB16 and Trichoderma longibrachiae 29TJ04

[0060] 1.1 Soil samples were collected from saline-alkali land in Tianjin and Hebei.

[0061] 1.2 Medium

[0062] Peel the potatoes, cut 200g into small pieces, add water to boil for 30min, filter through 4 layers of gauze, add 20g of glucose, distill water to 1000mL, boil and mix to obtain a PD liquid medium without sodium chloride.

[0063] Peel the potatoes, cut 200g into small pieces, add water to boil for 30min, filter with 4 layers of gauze, add 20g of glucose, 20g of agar, distilled water to a volume of 1000mL, boil and mix to obtain a sodium chloride-free PDA medium.

[0064] Saline-alkali-tolerant Trichoderma selective solid medium: Add sodium chloride to the above-mentioned PDA medium without sodium chloride until the content of sodium chloride is 20g / L (0.34mol / L), 50g / L (0.86 mol / L) or 80g / L (1.4mol / L), the pH was adjusted to 8.0 to obtai...

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Abstract

The invention discloses saline-alkali tolerant trichoderma harzianum and application thereof. The strain number of the saline-alkali tolerant trichoderma harzianum disclosed by the invention is 29HB16; the accession number of the saline-alkali tolerant trichoderma harzianum in a general microorganism center of the China Committee for Culture Collection of Microorganisms is CGMCC No.7975. The trichoderma longibrachiatum 29HB16 disclosed by the invention can tolerate salt stress caused by less than 80 g/L of salinity, and alkali stress of which pH is less than 8.0; the trichoderma harzianum 29HB16 disclosed by the invention is imported to saline alkali soil; a fungal disease can be effectively prevented and treated by biological control; an environment can be effectively improved; balance of an agricultural ecological system is maintained.

Description

technical field [0001] The invention relates to a saline-alkali-resistant Trichoderma harzianum and its application. Background technique [0002] Trichoderma (Trichoderma spp.) belongs to the half-known fungi of the class Hyphophyceae, the order Polypodium, and the family Polypodiaceae, and is widely present in the soil. In 1932, Weindling discovered that Trichoderma lignorum can parasitize a variety of plant pathogenic fungi, and suggested that the fungus be used for the biological control of soil-borne plant diseases, and the research on the biocontrol of Trichoderma began from then on. Since the 1970s, scholars at home and abroad have done a lot of in-depth research on the antagonism of Trichoderma and its mechanism. K.Rinu (2013) et al. found that the volatile substances produced by Trichoderma can cause structural distortion of pathogenic bacteria, and then cause morphological distortion, thereby inhibiting the growth of its spores. Research by Li Chongwei (2012) poi...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 顾金刚李世贵王倩崔西苓雷强屈健康刘好宝张良龚明波
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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