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Strain capable of massively yielding glucose-6-acetic ester and method for synthesizing glucose-6-acetic ester

A technology of glucose and acetate, applied in the field of biochemical engineering, can solve the problems of poor passage stability, low yield, unsuitable for industrial production and the like

Inactive Publication Date: 2015-02-25
防城港市润杰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used production method of glucose-6-acetate is obtained by using microbial fermentation method, and the strain used is ATCC10778, but the yield of this strain is not high, the fermentation time is long and the passage stability is poor, so it is not suitable for large-scale industrial production

Method used

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  • Strain capable of massively yielding glucose-6-acetic ester and method for synthesizing glucose-6-acetic ester
  • Strain capable of massively yielding glucose-6-acetic ester and method for synthesizing glucose-6-acetic ester
  • Strain capable of massively yielding glucose-6-acetic ester and method for synthesizing glucose-6-acetic ester

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The steps of the fermentation separation method for producing glucose-6-acetate are as follows:

[0057] (1) Bacterial slant culture

[0058] Bacillus sp. (Bacillus sp.) strain GXU-011 bacterial classification is inoculated into the slant culture medium in the test tube to make the slant bacterial classification;

[0059] Wherein the bacterial strain slant culture medium is made up of following components by weight:

[0060] Sucrose 40g, yeast powder 2g, KH 2 PO 4 0.5g, K 2 HPO 4 0.5g, MgSO 4 0.5g, agar powder 15g and water 1000 parts;

[0061] The technological conditions for preparing slant strains are pH 6.5, temperature 28° C., and incubation time 24 hours.

[0062] (2) Inoculation fermentation

[0063] Expand and cultivate the slant strains into seed liquid;

[0064] The seed liquid is inserted in the fermenter by 5% inoculum size and fermented;

[0065] Wherein the seed liquid and the fermented liquid consist of the following components in parts by weigh...

Embodiment 2

[0076] The steps of the fermentation separation method for producing glucose-6-acetate are as follows:

[0077] (1) Bacterial slant culture

[0078] Bacillus sp. (Bacillus sp.) strain GXU-011 bacterial classification is inoculated into the slant culture medium in the test tube to make the slant bacterial classification;

[0079] Wherein the bacterial strain slant culture medium is made up of following components by weight:

[0080] 50g of sucrose, 6g of yeast powder, KH 2 PO 4 2g, K 2 HPO 4 2g, MgSO 4 2g, agar powder 20g and water 1000 parts;

[0081] The technological conditions for preparing the slant strain are pH value 7.0, temperature 30° C., and incubation time 30 hours.

[0082] (2) Inoculation fermentation

[0083] Expand and cultivate the slant strains into seed liquid;

[0084] The seed liquid is inserted in the fermenter by 8% inoculum size and fermented;

[0085] Wherein the seed liquid and the fermented liquid consist of the following components in parts...

Embodiment 3

[0093] The steps of the fermentation separation method for producing glucose-6-acetate are as follows:

[0094] (1) Bacterial slant culture

[0095] Bacillus sp. (Bacillus sp.) strain GXU-011 bacterial classification is inoculated into the slant culture medium in the test tube to make the slant bacterial classification;

[0096] Wherein the bacterial strain slant culture medium is made up of following components by weight:

[0097] Sucrose 60g, yeast powder 8g, KH 2 PO 4 1.5g, K 2 HPO 4 1.5g, MgSO 4 1.5g, 25g of agar powder and 1000 parts of water;

[0098] The technological conditions for preparing slant strains are pH value 7.5, temperature 32° C., and incubation time 36 hours.

[0099] (2) Inoculation fermentation

[0100] Expand and cultivate the slant strains into seed liquid;

[0101] The seed liquid is inserted in the fermenter by 10% inoculum size and fermented;

[0102] Wherein the seed liquid and the fermented liquid consist of the following components in par...

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Abstract

The invention discloses a strain capable of massively yielding glucose-6-acetic ester and a method for synthesizing the glucose-6-acetic ester. The collection number of Bacillus sp. GXU-011 is CGMCC No.7031; the Bacillus sp. GXU-011 is collected in the China general microbiological culture collection center; the collection date is December 24, 2012; the collection address is Beijing institute of microbiology, Chinese Academy of Sciences. The method comprises the following steps of: culturing organic nutrients, namely a carbon source, a nitrogen source, inorganic salt, and the like to obtain reaction liquid glucose-6-acetic ester; then separating and purifying the reaction liquid glucose-6-acetic ester to obtain the glucose-6-acetic ester. Compared with the traditional strain ATCC10778, the strain disclosed by the invention has the advantages of short fermentation time, simple process step, high output and high passage stability.

Description

technical field [0001] The invention belongs to the field of biochemical engineering, in particular to a high-yield glucose-6-acetate strain and a method for synthesizing glucose-6-acetate. Background technique [0002] The invention relates to the preparation of key intermediate glucose-6-acetate for producing sucralose by biological fermentation. Glucose-6-acetate is a key intermediate in the production of 4,1’,6’-trichloro-4,1’,6’-trideoxygalactose, i.e. sucralose. Sucralose is a chlorine derivative of sucrose. Its sweetness is 600-800 times that of sucrose. Its taste is mellow, rich, stable and low in calories. It can be used in combination with traditional sweeteners or alone. Its sweet taste is not affected, which is beneficial to the health of consumers. Glucose-6-acetate is a key intermediate in the synthesis of sucralose. At present, the commonly used production method of glucose-6-acetate is obtained by microbial fermentation, and the strain used is ATCC10778, b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/68C12Q1/04C12P19/02C12R1/07
Inventor 李群良张欣邓子明
Owner 防城港市润杰生物科技有限公司
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