Preparation method of S-adenosylmethionine

Abstract

The invention relates to a preparation method of S-adenosylmethionine. The method comprises the steps of: (1) fermentation: fermenting Escherichia coli for 12h to obtain a thallus; keeping the fermentation level at 100g / L, conducting thallus breaking to obtain a bacterium suspended solution, adding water into trihytdroxy methyl-aminomethane, performing stirring dissolving, carrying out dilution to 1000ml, using a hydrochloric acid solution to adjust the pH value to 8.0 to obtain a Tris-Hcl buffer solution, adjusting the thallus concentration according to the performance of a high pressure homogenizer, and performing microscopic inspection to find that the thallus breaking rate is greater than 90%; (2) enzyme supernatant collection: employing a low temperature high speed centrifuge, carrying out enzymatic reaction, performing rough filtration to recover enzyme, using a hollow fiber column to remove enzyme, performing nanofiltration concentration and ion exchange, concentrating a purification solution to about 350L by a nanofilter; and (3) resin adsorption: detecting purity by HPLC, carrying out 1, 4-butanedisulfonate anion detection, and calculating the content, then performing spray drying so as to obtain an S-adenosylmethionine finished product. The method provided by the invention has the advantages of simple process, low cost and suitability for mass production, and the obtained product is more stable.

Description

technical field [0001] The invention belongs to the field of medicine, in particular to a preparation method of S-adenosylmethionine. Background technique [0002] S-adenosylmethionine is abbreviated as SAM. It is a life supplement that integrates medical treatment and nutrition and health care. It has significant therapeutic and health care effects on depression, liver disease, arthritis and anti-aging. Clinical applications and pharmacological studies have shown that SAM is the only methyl group donor in the human body and is involved in the biosynthesis and metabolism of hormones, neurotransmitters, nucleic acids, proteins and phospholipids in the body. It is indispensable for maintaining the normal function of cell membranes and normal human metabolism and health. important living substances. Since SAM is not currently produced in China, it is completely dependent on imports, and the traditional process is very expensive, making it very expensive. In particular, the raw...

AI Technical Summary

Problems solved by technology

Since SAM is not produced in China at present, it is completely dependent on imports, and the high cost of traditional technology makes the price very expensive, especially the raw material of SAM is extremely unstable, which is greatly affected by pH value, temperature and humidity. In the production of raw materials and preparations It has been seriously affected, restricting the popularization and use of SAM, far from meeting social needs