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Detection method for internal bacteria in cyclops zooplankton

A technology for zooplankton and detection methods, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of distorted detection results, low detection results, and low bacterial concentration, and achieve significant detection and accurate detection results. , the effect of high sensitivity

Inactive Publication Date: 2015-01-14
HOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has two main disadvantages: firstly, the use of liquid chlorine disinfection will cause the disinfectant molecules to penetrate into the zooplankton, which will have a certain inactivation effect on the bacteria in the zooplankton, which will cause distortion of the test results; secondly, the ultrasonic cell disruptor has With the characteristics of high power and high frequency, it can crush tissues, bacteria, viruses, spores and other cell structures, so it can kill some bacteria in the body during the ultrasonic crushing process, and it will also cause low test results
In addition, since most of the bacteria in the body are concentrated in the intestinal tract of zooplankton, if the bacteria are not released from the intestinal tract, the concentration of bacteria in the body of the zooplankton released into the water after ultrasonic crushing will be low, and the test results will also be biased. Low

Method used

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  • Detection method for internal bacteria in cyclops zooplankton
  • Detection method for internal bacteria in cyclops zooplankton

Examples

Experimental program
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Effect test

Embodiment 1

[0034] A kind of detection method of the bacterium in the Cyclops class zooplankton, comprises the following steps:

[0035] (1) Use a 3mL Pasteur pipette to draw 15 adult Cyclops one by one from the water sample, put them into a 60mm sterile petri dish filled with 100mL sterile water, and place them on a sterile operating table.

[0036] (2) Carry out ultraviolet irradiation on Daphnia xenophobia in the petri dish, the ultraviolet wavelength is 190nm, and the ultraviolet intensity at the liquid surface of the petri dish is 2000μW / cm 2 , The irradiation time is 20min, and the free bacteria in the water and the bacteria attached to the body surface of Cyclops are inactivated.

[0037] (3) After inactivation, transfer Daphnia xenophobia to a 1.5mL sterile centrifuge tube, add 1mL sterile water, place in a refrigerator at -8°C for 1 hour, take it out and thaw it at 15°C for 10 minutes , and then use an ultrasonic cleaner (power 60W, frequency 40kHz) to perform ultrasonic action ...

Embodiment 2

[0042] A kind of detection method of the bacterium in the Cyclops class zooplankton, comprises the following steps:

[0043] (1) Use a 3mL Pasteur pipette to draw 15 adult Cyclops one by one from the water sample, put them into a 60mm sterile petri dish filled with 100mL sterile water, and place them on a sterile operating table.

[0044] (2) UV irradiation is carried out on Daphnia xenophobia in the petri dish, the ultraviolet wavelength is 350nm, and the ultraviolet intensity at the liquid surface of the petri dish is 4200μW / cm 2 , The irradiation time is 20min, and the free bacteria in the water and the bacteria attached to the body surface of Cyclops are inactivated.

[0045](3) After the inactivation is completed, transfer the water flea to a 1.5mL sterile centrifuge tube, add 1mL sterile water, and place it in a refrigerator at -10°C for 1 hour, then take it out and thaw it at 25°C for 10 minutes , and then use an ultrasonic cleaner (power 60W, frequency 40kHz) for 2 mi...

Embodiment 3

[0050] A kind of detection method of the bacterium in the Cyclops class zooplankton, comprises the following steps:

[0051] (1) Use a 3mL Pasteur pipette to draw 15 adult Cyclops one by one from the water sample, put them into a 60mm sterile petri dish filled with 100mL sterile water, and place them on a sterile operating table.

[0052] (2) Carry out ultraviolet irradiation on Daphnia xenophobia in the petri dish, the ultraviolet wavelength is 200nm, and the ultraviolet intensity at the liquid surface of the petri dish is 2500μW / cm 2 , The irradiation time is 30min, and the free bacteria in the water and the bacteria attached to the body surface of Cyclops are inactivated.

[0053] (3) After the inactivation is completed, transfer the water flea to a 1.5mL sterile centrifuge tube, add 1mL sterile water, place it in a refrigerator at -10°C for 1 hour, and then take it out and thaw it at 20°C for 20 minutes , and then use an ultrasonic cleaner (power 60W, frequency 40kHz) for...

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Abstract

The invention discloses a detection method for internal bacteria in cyclops zooplankton. Free bacteria and bacteria attached to the body surfaces of cyclops in a water sample are inactivated by ultraviolet light. On the premise of effectively inactivating the free bacteria and bacteria attached to the body surfaces of the cyclops in water, because of sheltering effect of the cyclops to the internal bacteria, the method solves the problem that internal microorganisms are affected by permeation of chlorine molecules in the process of inactivating the bacteria attached to the body surfaces of the cyclops by using liquid chlorine in existing detection methods. Cyclops body structure is crushed by a freeze-thaw method and an ultrasonic method, and a desorption reagent is additionally added to elute the internal bacteria attached to organs such as intestinal tract. Finally, bacteria are cultivated and counted according to a testing method of total number of bacteria in household drinking water standard test method (GB / T5750.12-2006), and the number of the internal bacteria is detected. The method disclosed by the invention has the advantages of high accuracy, reliability and the like, and has a wide application prospect.

Description

technical field [0001] The invention relates to a method for detecting bacteria in the body of Cyclops zooplankton in a drinking water treatment process. technical background [0002] In recent years, there have been frequent incidents of hydrozoan zooplankton penetrating the drinking water treatment process system and entering the pipe network in China, which has threatened and challenged the safety of drinking water. The practice of the water plant has proved that the water treatment process cannot completely remove the water flea, and the adult body of the water flea is visible to the naked eye, and its appearance in the water treatment process system brings about sensory problems for users. At the same time, Daphnia can be used as a carrier of bacteria or an intermediate host for other pathogenic protozoa, and bacteria can exist stably on the surface and body of Daphnia; Daphnia can provide shelter for the bacteria it carries, causing Water quality biosecurity issues. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/06
Inventor 林涛陈卫蔡博吴寿可
Owner HOHAI UNIV
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