Method for evaluating potential of complexation of water-soluble humus matter with heavy metals

Abstract

A method for evaluating the potential of the complexation of a water-soluble humus matter with heavy metals mainly comprises the steps of (A) extracting and preparing water-soluble organic samples, scanning three-dimensional fluorescence spectrums of the organic samples, deducting background values and then exporting three-dimensional fluorescence data; (B) carrying out parallel factor analysis on the exported three-dimensional fluorescence data on MATLAB by adopting DOMFluor software so as to obtain the number of fluorescence components and the concentration score Fmax of each sample in the corresponding fluorescence component; (C) adding the Fmax of the fluorescence components with the emission wavelength more than 380nm at a fluorescence peak center so as to obtain the concentration score F1 of the water-soluble humus matter, adding the Fmax of the fluorescence components with the emission wavelength more than 420nm at the fluorescence peak center to obtain the score F2, and calculating according to HQ=F2/F1 to obtain HQ, and judging the potential of complexation of the water-soluble humus matter with heavy metals through the HQ, wherein if the HQ value of the water-soluble humus matter is large, the potential of complexation with heavy metal is high, and if the HQ value of the water-soluble humus matter is small, the potential of complexation with heavy metal is low. According to the method, the calculation is simple, the analysis is quick, and the sensitivity is high.

Description

technical field [0001] The invention belongs to the technical field of heavy metal pollution and restoration, and in particular relates to an evaluation method for the potential of complexing heavy metals with water-soluble humic substances. Background technique [0002] Humic matter is a common natural organic matter widely distributed in soil, water, and organic waste, and its composition includes water-insoluble humin, and water-soluble humic acid and fulvic acid. Humus contains a large number of functional groups such as hydroxyl, carboxyl, and carbonyl, which can complex heavy metals and affect the existence, migration, transformation, and bioavailability of heavy metals in the environment. Compared with water-insoluble humin, water-soluble humus has strong mobility and high bioavailability, and has a greater impact on the environmental behavior of heavy metals. Therefore, clarifying the complexation potential of water-soluble humus to heavy metals is important for the ...

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Problems solved by technology

On the other hand, the complexing potential of water-soluble humic substances to heavy metals, including its complexing force and complexing capacity, is determined by its structure and functional group composition. If the benzene ring functional groups of water-soluble humic substances are exposed to the solution, the functional groups on it will For electron-donating groups such as hydroxyl and amino groups, the center of the fluorescence peak will be red-shifted, the fluorescence intensity will be enhanced, and there will be many complexation sites for heavy metals, and the complexation potential is high. On the contrary, if the benzene ring functional groups on the humic substance are all wrapped up , which are mainly electron-withdrawing groups such as carboxyl and carbon groups, the center of the fluorescence peak is blue-shifted, the fluorescence intensity is low, and it can provide few heavy metal binding sites and low potential for complexing heavy metals. Therefore, it can be based on water-soluble humic substances. However, there is no relevant report to develop a method based on the fluorescence characteristics of water-soluble humic substances to evaluate its heavy metal complexing potential.

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