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Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings

A technique for transplanting survival rate and group of T. chinensis is applied in the field of transplanting survival rate of T. chinensis tissue culture seedlings. Failure to apply tissue culture and rapid propagation technology to achieve the effect of good growth of new shoots, improved seedling hardening methods, and increased survival rate

Inactive Publication Date: 2015-04-08
湖南省农业生物资源利用研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the tissue culture technology of Trichosanthes has become mature, but the artificial large-scale planting of Trichosanthes is still in its infancy in my country, and the development speed is relatively slow. The 1-2 months after the bottle was released was mainly due to the large difference between the environment of the tissue culture seedlings in the culture room and the transplanting growth environment, which seriously affected the transplanting survival rate of the Trichosanthes tissue culture seedlings, and failed to make the tissue culture rapid propagation. Technology applied to production practice
[0004] At present, almost no scientific research institutes and enterprises have carried out relevant research on the hardening, domestication and transplanting substrates of Trichosanthes seedlings

Method used

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  • Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings
  • Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0034] A method for improving the survival rate of Trichosanthes tissue culture seedling transplanting:

[0035] (1) Tissue culture:

[0036] Tissue culture of Trichosanthes seedlings in the room according to conventional methods (References: Yang Lina, Liu Jie, Tao Jianmin. Tissue culture and rapid propagation of Trichosanthes [J]. Jiangsu Agricultural Science, 2008, 15(4): 89-90.) ,

[0037] In this example, the formulation of Trichopina rooting medium is MS+0.2 (mg / L) NAA.

[0038] (2) Seedling hardening in a bottle: On March 20, 2013, 50 seedlings with a height of 3-5cm, 2-3 leaves, and 3-5 roots were put into a greenhouse to harden seedlings for 7 days. , and then open the culture bottle cap to harden the seedlings, add an appropriate amount of distilled water in the culture bottle to immerse the roots of Trichosanthes chinensis, keep the air humidity greater than 70%, and place it for 3 days.

[0039] (3) Seedling hardening outside the bottle

[0040] Substrate prepa...

Embodiment 2

[0051] Conventional Trichosanthes tissue culture seedling transplanting method:

[0052] Same as Example 1, the difference is that the tissue-cultured seedlings are placed under natural conditions to harden the seedlings for 5-7 days, then open the culture bottle cap and then harden the seedlings for 3-5 days, and then directly transplant them into the field. Choose 50 strains to plant in the plant of above-mentioned refined seedling, altogether 8 strains survive, and survival rate is only 16%.

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Abstract

The invention discloses a method for improving the transplanting survival rate of trichosanthes kirilowii tissue culture seedlings. According to the method, the antibacterial ability of the trichosanthes kirilowii tissue culture seedlings is enhanced by proportioning different substrate varieties with different ratios, seedling-hardening and domesticating processing, transplanting and managing in a restoring growing period after the transplanting. By the method disclosed by the invention, the trichosanthes kirilowii tissue culture seedlings are basically suitable for the external large-land growth environment, the plant root system is thick and strong, the new sprouts are good in growing vigor, the rattans are robust, and the transplanting survival rate can achieve 80-95% which is greatly improved compared with the survival rate of 10-20% of the traditional method. The method is convenient to operate, low in price and suitable for processing in batches and industrialized production, and has excellent economical efficiency and social benefits.

Description

technical field [0001] The invention belongs to the field of cultivating seedlings of plant groups, and in particular relates to a method for improving the survival rate of transplanted seedlings of Trichosanthes group culture by hardening seedlings, preparing substrates, transplanting and managing after transplanting. Background technique [0002] Trichosanthes kirilowii Maxim is a perennial herbaceous vine of Cucurbitaceae Trichosanthes genus, also known as Trichosanthes kirilowii. It is a dioecious plant and is a traditional Chinese medicinal material commonly used in my country. Trichosanthes whole body is full of treasures, its fruit and roots can be used as medicine, the seeds are called Trichosanthes kernel, and the root is called Trichosanthes pollen, which has the effects of clearing away heat and resolving phlegm, widening chest and dispelling stagnation, moistening dryness and smoothing intestines, reducing swelling and discharging pus. Edible Trichosanthes seeds ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01G31/00
Inventor 黄艳宁彭斯文彭福元朱校奇
Owner 湖南省农业生物资源利用研究所