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A kind of preparation method of chicken infectious bursal disease compound live vaccine

A compound live vaccine and technology for bursal disease, which is applied in the field of preparation of chicken infectious bursal disease compound live vaccine, can solve the problems of ineffective immunity, inability to cause cellular immunity, and high production costs, and improve the prevention of diseases The ability to improve humoral immunity and save manpower and material resources

Active Publication Date: 2015-09-30
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because of its strong virulence, live vaccines can easily cause damage to the bursa of Fabricius, leading to immunosuppressive diseases, and also affect the immune failure of other vaccines
The scope of use of inactivated vaccines is relatively narrow, only suitable for breeders, and cannot cause cellular immunity, and the immune effect is not obvious
At present, chicken infectious bursal disease antigen-antibody complex vaccines include chicken infectious bursal disease compound freeze-dried live vaccine (W2512G-61 strain), chicken infectious bursal disease compound freeze-dried live vaccine (2512 strains), and The production method is the traditional method of combining hyperimmune serum with bursal virus. This method requires a large amount of hyperimmune serum antibodies to be prepared by slaughtering a large number of animals, which is time-consuming and laborious. At the same time, the production cost is high, resulting in a relatively expensive vaccine

Method used

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  • A kind of preparation method of chicken infectious bursal disease compound live vaccine
  • A kind of preparation method of chicken infectious bursal disease compound live vaccine
  • A kind of preparation method of chicken infectious bursal disease compound live vaccine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 uses DF-1 cell line to cultivate chicken infectious bursal virus to prepare chicken infectious bursal virus suspension, comprising the following steps:

[0039] (1) Passage and culture of cells for seedling production: take chicken embryo fibroblast passage cell line DF-1 cell line, digest and passage with trypsin cell dispersion liquid, and continue to culture with cell growth liquid. When a good monolayer is formed, it is used to continue passaging or inoculation with virus;

[0040] (2) Propagation of cytotoxic seeds: Take the infectious bursal virus B87 strain (purchased from the China Veterinary Drug Administration) virus seeds for production, and inoculate 1% of the maintenance fluid volume in chickens that have grown into a good monolayer. Place embryonic fibroblast passage cells in the maintenance medium at 37-38°C to continue culturing, and harvest the cell fluid when the cell lesion rate reaches 75% or more;

[0041] (3) Propagation of venom for ...

Embodiment 2

[0043] Embodiment 2 The preparation of chicken infectious bursal disease egg yolk antibody comprises the following steps:

[0044] a. Immune healthy laying hens with chicken infectious bursa inactivated vaccine. The immunization program is the first chest muscle immunization 0.5ml / bird; the second immunization one week after the first immunization, chest muscle immunization 1.0ml / bird; the third immunization two weeks after the second immunization, chest muscle immunization 1.0ml / bird.

[0045] b. Two weeks after the third immunization, start to monitor the antibody titer of high-immunity eggs. When the agar diffusion titer of anti-porcine reproductive and respiratory syndrome virus yolk antibody in the high-immunity eggs reaches 1:128, collect qualified high-immunity eggs.

[0046] c. Separation of egg yolk: Clean and disinfect the undamaged high-free eggs collected in b with 0.1% bromogeramine, break the shell and separate the egg yolk, collect the egg yolk in the egg yolk s...

Embodiment 3

[0050] The preparation of embodiment 3 traditional Chinese medicine polysaccharide comprises the following steps:

[0051] a. Weigh each Chinese medicine raw material according to 20-40 parts of Achyranthes bidentata, 10-30 parts of Poria cocos, 10-30 parts of epimedium, 5-20 parts of nettle, 10-30 parts of jingjing, and 5-20 parts of salvia miltiorrhiza, After chopping and washing, soak in cold water overnight, then add purified water 15 times the weight of raw materials, water bath to 90°C, and keep at 90°C, cook for 2 hours, stir every 10 minutes during the cooking process;

[0052] b. Discard the dregs of the traditional Chinese medicine in a, collect the medicinal liquid, and centrifuge at 10,000 rpm for 10 minutes after cooling at room temperature, discard the precipitate, and collect the supernatant;

[0053] c. Precipitate the supernatant in b with alcohol, and obtain the crude Chinese medicine polysaccharide by precipitation;

[0054] d. Wash the crude traditional Ch...

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Abstract

The invention discloses a method for preparing a chicken infection bursal disease composite live vaccine, belonging to the technical field of animal biological products. The method comprises the following process steps: (1) abdicating and culturing cells for preparing the vaccine; (2) breeding cellular poison; (3) breeding venom for preparing the vaccine; (4) preparing an antigen-antibody complex; and (5) preparing a finished product. When the method is adopted for preparing the vaccine, the production process is simple and stable, the operation is easy to carry out, the quality is easy to control, the vaccine quality is remarkably improved, and the production cost is remarkably lowered; and the prepared chicken infection bursal disease composite live vaccine is good in security and high in immune effect, and a comprehensive immune protection effect on attack of infection bursal disease viruses is achieved.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a preparation method of a chicken infectious bursal disease compound live vaccine. Background technique [0002] With the rise of intensive poultry farming and the increase of international trade, poultry diseases are becoming more and more serious, resulting in high mortality of poultry, which seriously restricts the development of the poultry industry, causes serious losses to the world's poultry industry, and endangers economic development, food security and social stability. Chicken infectious bursal disease (Infection bursal disease, IBD) is a highly contagious disease of chickens and turkeys caused by infectious bursal virus (Infection bursal disease virus, IBDV). For young chickens at the age of one week, the virus mainly proliferates in the lymphocytes of the bursa of Fabricius, and causes varying degrees of damage to other immune organ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/42A61P31/14A61K39/12A61K36/537
Inventor 李阳闫艳丽刘雪王二先徐晓艳盛璐丝
Owner 浙江美保龙生物技术有限公司