Molecule marking method for pig backfat thickness property

A technology of molecular markers and pig backfat thickness, which is applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of low accuracy and blindness in seed selection, achieve simple operation, speed up the selection process, The effect of shortening the generation interval

Inactive Publication Date: 2014-01-08
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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Problems solved by technology

[0008] The present invention designs the 17th exon primer of porcine ACSL1 gene, establishes the detection method of the 17th exon T46C site mutant, and analyzes the correlation between its polymorphism and backfat thickness, and esta

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  • Molecule marking method for pig backfat thickness property
  • Molecule marking method for pig backfat thickness property
  • Molecule marking method for pig backfat thickness property

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Embodiment 1

[0069] See Table 3 for the polymorphism detection results of the long-chain acyl-CoA synthetase 1 (ACSL1) gene of 287 Huai pig new strain II line fourth-generation sows. The T allele frequency was 0.472, and the C allele frequency was 0.528. There were abundant polymorphisms at this site in this pig herd.

[0070] Table 3 Genetic parameters of the ACSL1 gene polymorphic site in the fourth generation of Huai pig new line II line

[0071]

[0072] Association analysis of long-chain acyl-CoA synthetase 1 (ACSL1) gene polymorphism and backfat thickness in a new line of Huai pig

[0073]The live backfat thickness at the penultimate rib was measured with a backfat measuring instrument at a body weight of 90kg, and the least squares analysis was carried out on the backfat thickness of 287 pigs by using the general linear model (GLM) program of SPSS13.0 software, and the gene effect The analysis was carried out and the results are shown in Table 4. The average backfat thickness o...

Embodiment 2

[0078] Yorkshire pigs are world-renowned lean-meat breeds, featuring fast growth, thin backfat, high lean meat percentage, and low feed-to-meat ratio. They are mainly used to produce hybrid sows.

[0079] The polymorphism detection results of the long-chain acyl-CoA synthetase 1 (ACSL1) gene of 239 large gram sows are shown in Table 5. The frequency of T allele and C allele was 0.565 and 0.435 in the Large York pig herd, and there were abundant polymorphisms at this locus in this herd.

[0080] Table 5 Genetic parameters of polymorphic sites of ACSL1 gene in Yorkshire pig herd

[0081]

[0082] Association analysis of long-chain acyl-CoA synthetase 1 (ACSL1) gene polymorphisms and backfat thickness in large gram pigs

[0083] The live body backfat thickness at the penultimate rib was measured with a backfat measuring instrument at a body weight of 90kg, and the least squares analysis was carried out on the backfat thickness of 239 large gram pigs by using the general linea...

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Abstract

The invention relates to a molecule marking method for pig backfat thickness property. The method comprises: extraction of pig genome DNA, design of a long-chain acyl-coenzyme Asynthetase 1 (ACSL1) gene seventh exon primer, amplification in vitro and genotype detection. For the first time, it is discovered that the polymorphism of the long-chain acyl-coenzyme Asynthetase 1 (ACSL1) gene seventeenth exon has significant correlation with the pig backfat thickness, and a method for detecting the restriction fragment polymorphism at mutation sites of the seventeenth exon is established. The molecule marking method is applicable to auxiliary selection on backfat thickness property during breeding of pigs, helps to realize early-stage breeding selection of boars, and even helps to accurately select breeding just when pigs are born, so that the generation interval is shortened, and the selection progress of the backfat thickness property is accelerated; and the method is simple in operation, condition requirements during a polymerase chain reaction are low, the length of amplified fragments is relatively short (292 bp), amplification is relatively easy, and the method helps to improve amplification efficiency and accuracy of genotype determining.

Description

technical field [0001] The invention belongs to the technical field of animal gene polymorphism, in particular to the gene polymorphism of porcine long-chain acyl-CoA synthetase 1 (ACSL1). Background technique [0002] Exogenous and endogenous fatty acids must be activated to enter their metabolic pathways, that is, to catalyze the synthesis of fatty acyl-CoA (Acyl-CoA), which is the first step in the utilization of fatty acids in mammals. ACS (Acyl-CoA Synthetase) is In the presence of CoA and ATP, free fatty acids are catalyzed to synthesize Acyl-COA (Piccini et al., 1998). The catalytic reaction is irreversible. The reaction is divided into two steps. The first step is to catalyze the combination of free fatty acids with adenosine triphosphate (ATP) to form acyl- Adenosine phosphate (Acyl-AMP) releases a pyrophosphate (Fatty acid+ATP→acyl-AMP+PPi); the second step is to catalyze the combination of Acyl-AMP and CoA to form Acyl-CoA, and release adenosine monophosphate (AMP...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李庆岗陶立刘林清
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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