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Plant stress tolerance related protein TaPK, and coding gene and application thereof

A gene and protein technology, which is applied to the plant stress tolerance-related protein TaPK and its encoding gene and application fields, can solve problems such as comprehensive improvement of stress tolerance of difficult plants.

Active Publication Date: 2015-03-04
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the stress tolerance of plants is a complex trait regulated by multiple genes, it is difficult to achieve comprehensive improvement of plant stress resistance by introducing a single functional protein gene

Method used

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  • Plant stress tolerance related protein TaPK, and coding gene and application thereof
  • Plant stress tolerance related protein TaPK, and coding gene and application thereof
  • Plant stress tolerance related protein TaPK, and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the cloning of TaPK

[0043] 1. Cloning of TaPK

[0044] Common wheat (Triticum aestivum L.) variety Triticum aestivum cv. Xiaobaimai grown in hydroponics for about 10 days can be obtained from the Institute of Crop Science, Chinese Academy of Agricultural Sciences, or from the National Germplasm Bank ( The serial number is ZM242); the literature referring to triticale is: Sun Haitao et al., Screening of interacting proteins of wheat TaDREB6 transcription factor, Chinese Agricultural Sciences, 2011, 44 (22): 4740-4747.; the literature mentioning triticale For: Isolation and molecular characterization of the Triticum aestivum L.ethylene-responsive factor 1 (TaERF 1) that increases multiple stress tolerance, Plant Mol Biol (2007) 65:719-732, Zhao Shi Xu, Lan Qin Xia, Ming Chen, Xian Guo Cheng, Rui Yue Zhang, Lian Cheng Li, Yun Xing Zhao, Yan Lu, Zhi Yong Ni, Li Liu, Zhi Gang Qiu, You Zhi Ma) The three-leaf stage seedlings were treated with NaCl for 2 hours,...

Embodiment 2

[0049] Embodiment 2, the impact of TaPK on plant stress tolerance

[0050] 1. Construction of recombinant expression vector

[0051] 1. Cloning of TaPK gene

[0052] A pair of primers (TaPK-121F and TaPK-121R) were designed according to the sequence of the TaPK gene, and SmaI and SpeI restriction sites were introduced at the ends of the primers respectively.

[0053] TaPK-121F: 5'-TTACCCGGGATGGACGAGGACGAGTACTCT-3' (SEQ ID NO: 3);

[0054] TaPK-121R: 5'-AGAACTAGTCGATCACAGCAGCTTCGG-3 (SEQ ID NO: 4).

[0055] The total RNA of white wheat leaves was extracted, cDNA obtained by reverse transcription was used as a template, and the above primers were used to amplify, and the obtained PCR product was subjected to 1.2% agarose gel electrophoresis, and its size was 1.8Kb. The PCR product is sent for sequencing, and the PCR product has the sequence 2 in the sequence listing, which is the TaPK gene.

[0056] Agarose Gel DNA Purification Kit Ver.2.0 (TaKaRa Company, Code No.: DV807A) ...

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Abstract

The invention discloses a plant stress tolerance related protein TaPK, and a coding gene and an application thereof. The protein is a protein (a) composed of an amino acid sequence represented by sequence 1 in a sequence table, or is a protein (b) obtained by substituting and / or deleting and / or adding one or more amino acid residues to the amino acid sequence represented by the sequence 1, related with the plant stress tolerance and derived from the sequence 1. The expression of the gene TaPK is induced at a low temperature, a high salt or a high temperature, and transgenic Arabidopis thaliana obtained by introducing the gene TaPK into Arabidopis thaliana has higher low temperature, high salt and high temperature resistances than wild Arabidopis thaliana. The protein and the gene provided in the invention provide bases for the artificial control of the stress resistance and stress tolerance related gene expression, and play a great part in the cultivation of stress resistance and stress tolerance enhanced plants.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a plant stress tolerance-related protein TaPK and its coding gene and application. Background technique [0002] Adversity stresses such as drought, high salinity and low temperature are obstacles to the growth and development of wheat. Therefore, understanding the response and signal transduction mechanism of wheat to stress conditions and improving the stress resistance of wheat varieties have become one of the important tasks of wheat genetic research and wheat variety improvement. [0003] Under adversity stress, plants will produce a series of responses, accompanied by many physiological, biochemical and developmental changes. Clarifying the response mechanism of plants to stress will provide scientific evidence for the research and application of stress-resistant genetic engineering. At present, the research on plant stress resistance has gradually penetrated into the cellula...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/12C12N15/54C12N15/63C12N5/10C12N1/21C12N15/11C12N15/82C12N15/84A01H5/00
CPCC12N9/1205C12N15/8271C12N15/8273
Inventor 徐兆师马有志刘沛裴丽丽李连城陈明
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI