Layering sequence fixing method for improving attached biomass of compound bacteria to surface of carrier

Abstract

The invention relates to a layering sequence fixing method for improving the attached biomass of compound bacteria to the surface of a carrier. The layering sequence fixing method comprises the following steps of dividing an activated carbon column into three layers, and respectively fixing three types of bacterial genus on the lower layer, the middle layer and the upper layer of the activated carbon according to the adsorption performance of the three types of bacterial genus from strong to weak and demands of the three types of bacterial genus for oxygen; pumping flora which is obtained through expansive cultivation into the sealed activated carbon column through a peristaltic pump, enabling bacterial liquid to flow through the activated carbon column from top to bottom under the gravity, and letting discharged water to flow back to a bacterial liquid bottle, wherein fixing is in a manner that the lower layer is fixed first, then the middle layer is fixed and finally the upper layer is fixed. The layering sequence fixing method has the advantages and the effects that with the adoption of the layering sequence fixing method, the operating is simple; the quantity of microorganisms fixed on the surface of the activated carbon is large; the activity of bacteria is high; different types of bacterial genus are distinguished on the activated carbon layers, so that the biodegradation and the utilization rate of the oxygen can be improved, and the starting period of a biological activated carbon fixing technology can be shortened.

Description

technical field [0001] The present invention relates to a layered and sequential immobilization method of bacterial flora, in particular to a layered sequential immobilization method for increasing the biomass of composite bacteria attached to the surface of a carrier, which can be used for the immobilization of bacterial flora in the process of immobilizing drinking water with biological activated carbon. Immobilization of an organic matter-degrading complex composed of Pseudomonas, Bacillus, and Brevibacterium on activated carbon. Background technique [0002] At present, in the drinking water immobilization biological activated carbon process, the method of immobilizing microorganisms on activated carbon is mainly by simply mixing microorganisms of different genus, expanding the culture, injecting them into the activated carbon column, and attaching the microorganisms to the activated carbon by physical adsorption. surface. This method does not consider the competition ...

AI Technical Summary

Problems solved by technology

This method does not consider the competition of different bacterial species for living space on the surface of activated carbon, the relationship between microbial activity and the adsorption performance of activated carbon, and the influence of environmental temperature, pH value, dissolved oxygen, circulation mode and other conditions on the biological effect of microorganisms immobilized on the surface of activated carbon. Therefore, the biomass on the immobilized activated carbon is less, and the biological activity is low, which directly affects the start-up and operation effect of the immobilized bioactivated carbon process and the long-term biological stability of the complex flora.

Images