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Method for rapidly determining contents of aureomycin for feed and impurities of aureomycin by HPLC

A technology for rapid determination of impurity content, applied in measuring devices, instruments, scientific instruments, etc., can solve the problem that the content and impurities of chlortetracycline in feed cannot be quickly, accurately and precisely completed, and the impurity detection of tetracycline and epitropic chlortetracycline cannot be completed. High test conditions are required to achieve the effect of fast detection, reduced labor intensity and cumbersome operation

Active Publication Date: 2014-01-15
PUCHENG CHIA TAI BIOCHEM
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Problems solved by technology

The tube-and-disk method objectively has the following defects: (1) The detection time is long and the procedures are cumbersome (at least 20 hours for one sample); (2) The test conditions are high (semi-sterile room); (3) The detection accuracy is not high (can be The confidence limit rate is 5%); (4) The use of indicator strains may easily cause biological contamination; (5) Impurities such as tetracycline and aureomycin cannot be detected
The tube-and-disk method cannot be used to quickly, accurately and accurately detect the content and impurities of aureomycin in feed

Method used

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  • Method for rapidly determining contents of aureomycin for feed and impurities of aureomycin by HPLC
  • Method for rapidly determining contents of aureomycin for feed and impurities of aureomycin by HPLC
  • Method for rapidly determining contents of aureomycin for feed and impurities of aureomycin by HPLC

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Embodiment Construction

[0031] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0032] The invention discloses a method for quickly measuring feed aureomycin and its impurity content by using HPLC, where HPLC is high-performance liquid chromatography. High-performance liquid chromatography is an important branch of chromatography, using liquid as the mobile phase, using a high-pressure infusion system to pump a single solvent with different polarities or a mixed solvent in different proportions, buffer and other mobile phases into a stationary phase. The chromatographic column, after the components in the column are separated, enters the detector for detection, thereby realizing the analysis of the sample. The method that this invention discloses uses HPLC to measure feed aureomycin and impurity content thereof comprises the f...

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Abstract

The invention discloses a method for rapidly determining contents of aureomycin for feed and impurities of aureomycin by HPLC. The method includes the following steps: preparing reagents and solutions, preparing chromatographic conditions, carrying out operational suitability test of HPLC system, and analyzing chromatograms. The method disclosed by the invention adopts HPLC precision detection instrument for determination, can determine contents of aureomycin for feed and chlorquatrimycin and tetracycline impurities simultaneously, and has the advantages of accurate determination results, rapid determination, and high determination precision.

Description

technical field [0001] The invention relates to the field of detection of chlortetracycline for feed (premix containing chlortetracycline antibiotic), in particular to a method for rapidly determining the content of chlortetracycline for feed and its impurity content by HPLC. Background technique [0002] Feed chlortetracycline is a feed chlortetracycline calcium salt made from the whole fermentation broth of chlortetracycline-producing bacteria and an appropriate amount of calcium carbonate. It is a feed chlortetracycline product made by drying, crushing and other processes. In the above-mentioned production process, the product will be accompanied by the generation of aureomycin group impurities, wherein the two most important impurities are tetracycline and epitrope aureomycin. [0003] At present, the detection method of chlortetracycline content in feed is carried out according to the second volume of "National Standards for Raising Local Standards of Veterinary Drugs"....

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Application Information

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IPC IPC(8): G01N30/02
Inventor 季红珍骆荣良
Owner PUCHENG CHIA TAI BIOCHEM
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