Method for simultaneously extracting chicoric acid, mono-caftaric acid, 3,5-dicaffeoylquinic acid and chlorogenic acid from pterocypsela indica as raw material

A technology of caffeoyl tartaric acid and caffeoyl quinic acid, which is applied in the field of chlorogenic acid, can solve problems such as limited root production, and achieve the effect of reducing raw material costs and production costs

Active Publication Date: 2014-01-22
NANJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amount of cichoric acid in the root of Echinacea purpurea is higher than that in the leaf (12.1mg / g), but the yield of the root is limited (about 0.45kg / m 2 ) (Wu Qilin, Research on the Extraction and Purification Technology of Chichoric Acid from Echinacea Purpurea, Beijing University of Chemical Technology, Master Thesis, 2004), so it is necessary to look for plant resources with higher content

Method used

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  • Method for simultaneously extracting chicoric acid, mono-caftaric acid, 3,5-dicaffeoylquinic acid and chlorogenic acid from pterocypsela indica as raw material
  • Method for simultaneously extracting chicoric acid, mono-caftaric acid, 3,5-dicaffeoylquinic acid and chlorogenic acid from pterocypsela indica as raw material
  • Method for simultaneously extracting chicoric acid, mono-caftaric acid, 3,5-dicaffeoylquinic acid and chlorogenic acid from pterocypsela indica as raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Take 1000g of the whole plant of Ptera multilobata and add 90% acetone according to the solid-to-liquid ratio of 1:30, and extract at 60°C for 2 hours (see HPLC analysis of the extract). figure 1 ) extract is concentrated and then extracted with n-butanol to obtain an extract containing chlorogenic acid and 3,5-dicaffeoylquinic acid and a raffinate containing cichoric acid and monocaffeoyltartaric acid;

[0041] The extract was separated and refined by conventional adsorption with D101 macroporous resin, and 90% of chlorogenic acid and 3,5-dicaffeoylquinic acid were obtained respectively.

[0042] The raffinate was separated and purified by conventional adsorption with XAD-2 resin to obtain cichoric acid and monocaffeoyl tartaric acid with a content of 90%.

Embodiment 2

[0044] Same as in Example 1, replace the whole plant of Ptera multilobata with the root of Ptera multilobata, add 60% methanol at a solid-to-liquid ratio of 1:100, extract at 90°C for 1.0 h, concentrate the extract with acetone Triol extraction, the extract is separated and refined by conventional adsorption with D301 macroporous resin, and 92% of chlorogenic acid and 3,5-dicaffeoylquinic acid are obtained respectively.

[0045] The raffinate was separated and refined by conventional adsorption with X-5 resin to obtain cichoric acid and monocaffeoyl tartaric acid with a content of 91%.

Embodiment 3

[0047] Same as in Example 1, the whole herb of Sachinacea multilobata was replaced with the leaves of Sachinacea multilobata. Add 70% ethanol at a solid-to-liquid ratio of 1:60, and extract at 60°C for 1 hour. The extract is concentrated and then extracted with isopropanol. After the extract is separated and refined by conventional adsorption with HPD100 resin, 89% of chlorogenic acid and 3,5-dicaffeoylquinic acid are obtained respectively.

[0048] The raffinate was separated and refined by conventional adsorption with HPD300 resin to obtain cichoric acid and monocaffeoyl tartaric acid with a content of 93%.

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Abstract

The invention discloses a method for simultaneously extracting chicoric acid, mono-caftaric acid, 3,5-dicaffeoylquinic acid and chlorogenic acid from pterocypsela indica as a raw material. After pterocypsela indica is extracted by a solvent (or in assistance of ultrasonic wave and microwave), the maximum extraction amounts of the four components, namely chicoric acid, mono-caftaric acid, 3,5-dicaffeoylquinic acid and chlorogenic acid, can respectively reach 25.3mg/g, 3.62mg/g, 6.0mg/g and 3.6mg/g, wherein the content of chicoric acid is the highest. The pterocypsela indica can serve as the raw material from which the natural products can be extracted. According to the method, the pterocypsela indica is effectively utilized; the pterocypsela indica as weeds to be removed becomes to a raw material which can be initiatively utilized; a scientific basis is provided for reasonably utilizing the pterocypsela indica; the method has the characteristics of rich sources and green processes.

Description

technical field [0001] The invention relates to a method for extracting and preparing active ingredients from plants, in particular to a method for extracting cichoric acid, monocaffeoyl tartaric acid, 3,5-dicaffeoyl quinic acid and chlorogenic acid from chrysanthemum chrysanthemum. Background technique [0002] Samara is an annual or perennial herb of the Compositae family. It used to be called Lactuca, and it is distributed in East and South Asia. There are tall Pterocypsela elata (Hemsl.) Shih, Taiwan Pterocypsela P.formosana (Maxim.) Shih, Pterocypsela P. triangulata (Maxim.) Shih, P. raddeana (Maxim.) .) Shih, P. sonchus (L évl.et Vaniot) Shih, P. indica (L.) C.Shih and P. laciniata (Houtt) Shih, etc. Yang Changxu published a new variety and newly combined a new variety when he was studying the genus Samantha in the Three Gorges Reservoir area. The new species is Pterocypsela lyrata C.X.Yang, and the new variant is Pterocypsela indica(L.)Shih var.dentata(Kom.)C.X.Yang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48
CPCC07C67/48C07C69/732
Inventor 陈育如柯小红闫莽钱媛媛
Owner NANJING NORMAL UNIVERSITY
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