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Method for preparing oriented polypeptide monomolecular film and product thereof

A monomolecular film, orientation technology, applied in piezoelectric effect/electrostrictive or magnetostrictive motors, metal material coating process, electrical components, etc., to achieve the effect of simple process, consistent molecular orientation, and easy operation

Active Publication Date: 2014-01-22
BEIJING INST OF NANOENERGY & NANOSYST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to solve the antiparallel structure that occurs when the traditional self-assembly method is used to prepare α-helical polypeptide monomolecular membranes, and to provide a new method for preparing oriented α-helical monomolecular membranes using piezoelectric potential generated by mechanical deformation, and Oriented α-helical polypeptide monomolecular film obtained by this method

Method used

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  • Method for preparing oriented polypeptide monomolecular film and product thereof
  • Method for preparing oriented polypeptide monomolecular film and product thereof
  • Method for preparing oriented polypeptide monomolecular film and product thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0058] In this example, the amino acid sequence of the α-helical polypeptide used is Leu 2 His Leu 6 His Leu 6 . And in order to promote the self-assembly of the above-mentioned polypeptide on the surface of a metal electrode such as a gold electrode of a nanogenerator, an organic sulfide lipoic acid is introduced into the N-terminus of the polypeptide. figure 1 A chemical formula is shown for a polypeptide that can be synthesized by conventional solid phase synthesis as follows:

[0059] 0.5 g of Clear-Acid Resin (Clear-Acid Resin, Peptide Institute Inc.) and 5 mL of dichloromethane were placed in a polypeptide solid-phase synthesizer and shaken for 24 hours to swell the resin. After fully washing the resin with N,N-dimethylformamide (DMF), 0.7 mM Fmoc-leucine (purchased from Peptide Institute Inc.) in DMF solution 3 mL, 0.7 mM N-hydroxy-7-azo Mix 1mL of DMF solution of benzotriazole with 1mL of DMF solution of 0.7mM 1,3-diisopropylcarbodiimide, add it into the polypeptid...

Embodiment 2

[0068] In this example, the amino acid sequence of the α-helical polypeptide used is Leu 2 His Leu 6 His Leu 6 . And in order to promote the self-assembly of the above-mentioned polypeptide on the surface of a metal electrode such as a gold electrode of a nanogenerator, a thiol is introduced into the C-terminus of the polypeptide. Figure 7 A chemical formula is shown for a polypeptide that can be synthesized by conventional solid phase synthesis as follows:

[0069] 0.5 g of coupled aminothiol resin (Resin-bound aminothiol) and 5 mL of dichloromethane were placed in a reaction vessel of a polypeptide solid-phase synthesizer and shaken for 24 hours to swell the resin. After fully washing the resin with N,N-dimethylformamide (DMF), add 0.7mM Fmoc-leucine DMF solution 3mL, 0.7mM N-hydroxy-7-azobenzotriazole DMF solution 1mL Mix with 1mL of 0.7mM 1,3-diisopropylcarbodiimide DMF solution, then add to the reaction vessel of the polypeptide solid-phase synthesizer to mix with th...

Embodiment 3～5

[0078] Oriented α-helical polypeptide monomolecular films were prepared in the same manner as in Example 1, except that the electrodes of the nanogenerators used were changed to copper, silver, or gold and silver electrodes respectively. As a result, an oriented α-helical polypeptide monomolecular film similar to that of Example 1 was obtained.

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Abstract

The invention relates to a method for preparing an oriented alpha-helical polypeptide monomolecular film and the oriented alpha-helical polypeptide monomolecular film obtained by the same. More specifically, the invention relates to the method for preparing the oriented alpha-helical polypeptide monomolecular film by utilizing a nano power generator. The method includes the steps: a first electrode or a second electrode of the nano power generator is immersed in an alpha-helical polypeptide solution, wherein a potential is generated between the first electrode and the second electrode of the nano power generator so that an alpha-helical polypeptide tail end is selectively immobilized on the electrode surface due to the electrostatic interaction between the immersed electrodes of the nano power generator, and thus the oriented alpha-helical polypeptide monomolecular film is obtained. The method has the advantages of simple process and easy operation; the prepared oriented alpha-helical polypeptide monomolecular film has stable alpha-helical structure and consistent molecular orientation; and moreover, the prepared oriented alpha-helical polypeptide monomolecular film can unidirectionally conduct electrons, and thereby reaching the objective of vector transmission of the electrons.

Description

technical field [0001] The invention relates to a method for preparing an oriented polypeptide monomolecular film, in particular to a method for preparing an oriented α-helical polypeptide monomolecular film by using a nanogenerator and the oriented α-helical polypeptide monomolecular film obtained by the method. Background technique [0002] Vector signaling, such as unidirectional electron transfer, is a fundamental feature of many biological systems. The generation and transformation of substances in living organisms are closely related to electrons transported by vectors. Artificial imitation of such an energy conversion system will not only help humans to better understand the nature of electron transfer in living organisms, but more importantly, vectorial electron transfer is of great significance in the fields of materials science and electronic engineering, and is of great help to solving energy and environmental problems. have profound impact. [0003] In recent y...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C23C26/00H02N2/18B82Y40/00
Inventor 李舟王心心
Owner BEIJING INST OF NANOENERGY & NANOSYST
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