AlphaLISA detection kit of penicillinase in milk product

Abstract

The invention provides an AlphaLISA detection kit of penicillinase in a milk product, and the AlphaLISA detection kit comprises a penicillinase standard product, biotin penicillinase, anti-penicillinase monoclonal antibody, an anti-mouse-IgG (immunoglobulin G) coated receptor magnetic bead, a streptavidin donor magnetic bead, a detection buffer solution, a white reaction plate, a sealing membrane and an operating specification. The anti-mouse-IgG coated receptor magnetic bead includes 100 mug / mL of a receptor magnetic bead, 0.05% of Proclin-300, and a phosphate buffer solution (PBS) with the PH of 7.4, and the streptavidin donor magnetic bead includes 100 mug / mL of a donor bead, 25mM of HEPES, 100mM of a NaCl solution and 0.05% of the Proclin-300. An AlphaLISA detection method of the penicillinase in the milk product is established by use of the AlphaLISA detection kit, and the method has the advantages of less sample use amount, high sensitivity, no washing, short detecting period, and the like.

Description

technical field [0001] The invention relates to the detection of penicillinase in milk products, in particular to an AlphaLISA detection kit for penicillinase in milk products. Background technique [0002] The problem of antibiotic residues in milk has attracted more and more attention from the international community. With the increase of national supervision on antibiotic residues in milk and dairy products, some unscrupulous traders illegally added penicillinase to milk to make milk pass the test. standard. Excessive use of penicillinase can cause allergic reactions in the human body, which is more likely to cause the abuse of antibiotics. Existing detection methods for penicillinase include iodometric method, acidity method, high performance liquid chromatography, enzyme-linked immunoassay, nitrocephalin chromogenic method and cup and saucer method. However, these methods have disadvantages such as cumbersome operation and low detection sensitivity. Especially for th...

AI Technical Summary

Problems solved by technology

However, these methods have disadvantages such as cumbersome operation and low detection sensitivity.

Especially for the cup and saucer method as a confirmation method, the operation process requires microbial culture, and then a series of concentration gradient standards are added to the Oxford cup. The detection time is not less than 3 to 5 days, and the throughput is extremely low.

Images