AlphaLISA detection kit of penicillinase in milk product

A technology of penicillinase and detection kit, which is applied in the direction of testing food, dairy products, material inspection products, etc., and can solve the problems of cumbersome operation, low detection sensitivity, and low throughput

Inactive Publication Date: 2014-01-22
INSPECTION & QUARANTINE TECH CENT OF CHONGQING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Claims
  • Application Information

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Problems solved by technology

However, these methods have disadvantages such as cumbersome operation and low detection sensitivity.
Especially for the cup and saucer method as a confirmation method, the operation process req

Method used

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  • AlphaLISA detection kit of penicillinase in milk product
  • AlphaLISA detection kit of penicillinase in milk product

Examples

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Embodiment Construction

[0012] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0013] The anti-penicillinase monoclonal antibody in the kit can be purchased from the market, or can be prepared by conventional methods for preparing monoclonal antibodies. Streptavidin donor magnetic beads include 100ug / mL donor magnetic beads, 25mM HEPES, 100mM NaCl solution and 0.05% Proclin-300. The detection buffer is selected from the buffer in the conventional detection kit.

[0014] 1. Preparation of anti-mouse IgG-coated receptor magnetic beads

[0015] 1. Wash acceptor magnetic beads: Take 5-50 μL (20 mg / mL) acceptor magnetic beads in a 1.5 mL centrifuge tube, add 50 μL of PBS (pH=7.4), centrifuge at 14000 r / min for 15 min, a...

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Abstract

The invention provides an AlphaLISA detection kit of penicillinase in a milk product, and the AlphaLISA detection kit comprises a penicillinase standard product, biotin penicillinase, anti-penicillinase monoclonal antibody, an anti-mouse-IgG (immunoglobulin G) coated receptor magnetic bead, a streptavidin donor magnetic bead, a detection buffer solution, a white reaction plate, a sealing membrane and an operating specification. The anti-mouse-IgG coated receptor magnetic bead includes 100 mug / mL of a receptor magnetic bead, 0.05% of Proclin-300, and a phosphate buffer solution (PBS) with the PH of 7.4, and the streptavidin donor magnetic bead includes 100 mug / mL of a donor bead, 25mM of HEPES, 100mM of a NaCl solution and 0.05% of the Proclin-300. An AlphaLISA detection method of the penicillinase in the milk product is established by use of the AlphaLISA detection kit, and the method has the advantages of less sample use amount, high sensitivity, no washing, short detecting period, and the like.

Description

technical field [0001] The invention relates to the detection of penicillinase in milk products, in particular to an AlphaLISA detection kit for penicillinase in milk products. Background technique [0002] The problem of antibiotic residues in milk has attracted more and more attention from the international community. With the increase of national supervision on antibiotic residues in milk and dairy products, some unscrupulous traders illegally added penicillinase to milk to make milk pass the test. standard. Excessive use of penicillinase can cause allergic reactions in the human body, which is more likely to cause the abuse of antibiotics. Existing detection methods for penicillinase include iodometric method, acidity method, high performance liquid chromatography, enzyme-linked immunoassay, nitrocephalin chromogenic method and cup and saucer method. However, these methods have disadvantages such as cumbersome operation and low detection sensitivity. Especially for th...

Claims

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Application Information

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IPC IPC(8): G01N33/04
Inventor 王昱聂福平杨俊李应国肖进文袁曾壮叶自霞
Owner INSPECTION & QUARANTINE TECH CENT OF CHONGQING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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