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A liver-type fatty acid-binding protein content detection kit and preparation method thereof

A fatty acid binding and detection kit technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of long detection time, large manual operation impact, low degree of automation, etc., to achieve good sensitivity, ensure stability, The effect of improving specificity

Active Publication Date: 2016-01-13
CUSABIO TECH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzyme-linked immunoassay technology has many excellent characteristics such as high detection accuracy and good sensitivity, but the detection method is cumbersome to operate and takes a long time to detect, which is not conducive to the timely detection of samples. At the same time, this detection method can only detect samples in batches, and cannot achieve In addition, the enzyme-linked immunoassay method has the disadvantages of low automation and great influence of manual operation, which makes it inevitably be replaced in the future detection technology.

Method used

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  • A liver-type fatty acid-binding protein content detection kit and preparation method thereof
  • A liver-type fatty acid-binding protein content detection kit and preparation method thereof
  • A liver-type fatty acid-binding protein content detection kit and preparation method thereof

Examples

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Effect test

Embodiment 1

[0035] Preparation of Latex Particle Solution Coated with Hepatic Fatty Acid Binding Protein Antibody:

[0036] (1) Preparation of carboxylated modified polystyrene latex solution

[0037] Take 0.5 mL of 10% polystyrene latex particles with a particle size of 200 nm (purchased from PolyMicrospheres) in a 50 mL centrifuge tube, and add 4.5 mL of 0.12 mol / L pH7.4 phosphate (PBS) buffer to the centrifuge tube , to obtain latex microspheres by centrifugation, wash the latex microspheres twice with deionized water, and then resuspend the latex microspheres with 5 mL of phosphate (PBS) buffer with a concentration of 0.12mol / LpH7.4; Add 1.0 mL of 0.02 mol / L 6-aminocaproic acid solution to the spherical particle solution, then add 2 mg of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), at room temperature Stir the reaction for 30 minutes, centrifuge and pour off the supernatant to make carboxylated polystyrene latex microspheres with glycine groups, and add 5 mL of phosphate (P...

Embodiment 2

[0052] Preparation of Latex Particle Solution Coated with Hepatic Fatty Acid Binding Protein Antibody:

[0053] (1) Preparation of carboxylated modified polystyrene latex solution

[0054] Take 0.5 mL of 10% polystyrene latex particles with a particle size of 200 nm (purchased from PolyMicrospheres) in a 50 mL centrifuge tube, and add 4.5 mL of 0.12 mol / L pH7.4 phosphate (PBS) buffer to the centrifuge tube , to obtain latex microspheres by centrifugation, wash the latex microspheres twice with deionized water, and then resuspend the latex microspheres with 5 mL of phosphate (PBS) buffer with a concentration of 0.12mol / LpH7.4; Add 1.0 mL of 0.02 mol / L 6-aminocaproic acid solution to the spherical particle solution, then add 2 mg of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), at room temperature Stir the reaction for 30 minutes, centrifuge and pour off the supernatant to make carboxylated polystyrene latex microspheres with glycine groups, and add 5 mL of phosphate (P...

Embodiment 3

[0069] Preparation of Latex Particle Solution Coated with Hepatic Fatty Acid Binding Protein Antibody:

[0070] (1) Preparation of carboxylated modified polystyrene latex solution

[0071] Take 0.4 mL of 10% polystyrene latex particles with a particle size of 220 nm (purchased from PolyMicrospheres) in a 50 mL centrifuge tube, and add 4.6 mL of 0.12 mol / L pH7.4 phosphate (PBS) buffer to the centrifuge tube , to obtain latex microspheres by centrifugation, wash the latex microspheres twice with deionized water, and then resuspend the latex microspheres with 5 mL of phosphate (PBS) buffer with a concentration of 0.12mol / LpH7.4; Add 0.8 mL of 0.02 mol / L 6-aminocaproic acid solution to the spherical particle solution, then add 1.8 mg of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), at room temperature Stir and react for 40 minutes, centrifuge and pour off the supernatant to make carboxylated polystyrene latex microspheres with aminoacetic acid groups, and buffer with 5 mL...

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Abstract

The invention discloses a liver fatty acid binding protein content detection kit and a preparation method of the liver fatty acid binding protein content detection kit and belongs to the technical field of detection of the content of liver fatty acid binding protein. The detection kit is composed of a reagent I and a reagent II which are independent of each other. The reagent I comprises a buffer solution, a preservative, a surfactant and water. The reagent II comprises latex particles coated with liver fatty acid binding protein antibodies, a buffer solution, a suspending agent, a preservative, a surfactant and water. The liver fatty acid binding protein content detection kit prepared according to the preparation method has the advantages of being easy to operate, accurate in quantification, high in sensitivity, good in specificity, wide in detection range and the like. The liver fatty acid binding protein content detection kit is suitable for popularization and application of liver fatty acid binding protein clinical detection.

Description

technical field [0001] The invention belongs to the technical field of detecting the content of liver-type fatty acid-binding protein, and in particular relates to a detection kit for the content of liver-type fatty acid-binding protein and a preparation method thereof. Background technique [0002] Liver fatty acid binding protein (liverfatty acid binding protein, L-FABP) is a member of the fatty acid binding protein family. It is a fatty acid binding protein expressed in the liver. It plays an important role in stress and affecting cell growth. In recent years, it has been found that L-FABP is a marker molecule of liver and kidney damage, and is closely related to the occurrence and development of fatty liver and obesity. With the deepening of research, the physiological function of L-FABP, gene regulation and the relationship with the disease have a clearer understanding, which is of greater help to the diagnosis and treatment of related diseases. [0003] Liver-type fa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/531
CPCG01N33/531G01N33/54313G01N33/68
Inventor 华权高来祥兵许可沈鹤霄舒芹
Owner CUSABIO TECH LLC