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Application of metrn1 protein in aspect of preparation of lipid-lowering drug

A blood lipid-lowering drug, 1. The technology of metrnl is applied in the field of medicine to achieve the effect of reducing blood lipid elevation, small side effects, and high safety

Active Publication Date: 2014-01-29
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no literature report on Metrn1 protein in the preparation of blood lipid-lowering drugs both at home and abroad

Method used

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  • Application of metrn1 protein in aspect of preparation of lipid-lowering drug
  • Application of metrn1 protein in aspect of preparation of lipid-lowering drug
  • Application of metrn1 protein in aspect of preparation of lipid-lowering drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Overexpression of Metrn1 can promote lipid droplet formation in adipocytes

[0022] Fat cell culture, the process is as follows:

[0023] The adipose precursor cell line 3T3-L1 was cultured in DMEM high-sugar plus 10% fetal bovine serum culture medium. The cells reached 80% confluence and were passaged. After being transfected with virus, they began to differentiate. The differentiation process is: continue to culture after cell fusion After 2 days, adipocyte differentiation medium was added, that is, 10% fetal bovine serum and 3-isobutyl-1-methylxanthine (IBMX, the total concentration was 0.115 mg / mL) were added to DMEM high-glucose medium. Dexamethasone (DEX, the total concentration is 1 μM), insulin (insulin, the total concentration is 1 μg / mL), and the medium is changed after two days of culture. , and continued to differentiate for 6 days, during which the medium was changed every two days. Lipid droplet formation and adipocyte differentiation were the...

Embodiment 2

[0030] Example 2: Metrn1 overexpression can promote the expression of differentiation genes in adipocytes

[0031] Fat cell culture, the process is as follows:

[0032] The adipose precursor cell line 3T3-L1 was cultured in DMEM high-sugar plus 10% fetal bovine serum culture medium. The cells reached 80% confluence and were passaged. After being transfected with virus, they began to differentiate. The differentiation process is: continue to culture after cell fusion After 2 days, adipocyte differentiation medium was added, that is, 10% fetal bovine serum and 3-isobutyl-1-methylxanthine (IBMX, the total concentration was 0.115 mg / mL) were added to DMEM high-glucose medium. Dexamethasone (DEX, the total concentration is 1 μM), insulin (insulin, the total concentration is 1 μg / mL), and the medium is changed after two days of culture. , and continued to differentiate for 6 days, during which the medium was changed every two days. Lipid droplet formation and adipocyte differentia...

Embodiment 3

[0036] Example 3: Preparation of Metrn1 fat-specific overexpression mice

[0037] First, the Metrn1 overexpression virus vector was prepared, and the preparation method was as follows: the open reading frame of the Metrn1 gene was cloned, the obtained sequence was inserted into the multi-cloning site of the lentiviral shuttle plasmid, and the shuttle plasmid and other packaging plasmids were co-transfected into HEK293 cells. The culture supernatant was then collected, filtered and centrifuged to purify and concentrate the virus.

[0038] The above-mentioned Metrn1 overexpression virus vector can also be obtained directly from commercial products, for example, the open reading frame of the Metrn1 gene can be purchased from Thermo Company, catalog number MMM1013-202763251. Viral packaging plasmids can be purchased from Invitrogen, Cat. No. K5325-20.

[0039] Construct the Metrnl adipocyte-specific overexpression mouse, and the culture method is as follows: construct the plasmid...

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Abstract

The invention relates to the technical field of medicines, and provides application of metrn1 protein in preparation of a lipid-lowering drug or a health-care food. An in vivo experiment shows that the blood lipid level of metrn1 fat cell specificity overexpression mice is obviously lower than that of normal mice when the normal mice and the metrn1 fat cell specificity overexpression mice are supplied with triglyceride lavage; the result shows that the metrn1 protein has an effect of reducing blood lipid. The metrn1 protein is intrinsic protein of a human body. Therefore, the safety of the metrn1 protein as a potential drug is high.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of Metrn1 protein in the preparation of blood lipid-lowering drugs. Background technique [0002] Metrnl protein is a very new protein, encoded by metrnl gene, with a size of about 30kDa. The amino acid sequence of human Metrnl protein is shown in NP_001004431.1. The expression and physiological function of this protein are almost unknown. [0003] Chinese patent application CN200980137344.4, the title of invention is "Therapeutic use of growth factor METRNL", application publication number: CN102164611A, discloses that METRNL is a neurosurvival and growth factor with neuron protection and / or neurogenesis, METRNL protein It can be used to treat diseases, disorders or damages of the nervous system. [0004] A large amount of evidence shows that hyperlipidemia is highly correlated with insulin resistance, and is an important factor that causes metabolic diseases s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61P3/06A23L1/305A23L33/18
Inventor 缪朝玉李志勇王培
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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