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Method for separating beta-sitosterol and stigmasterol from mixed phytosterol

A technology for phytosterol and stigmasterol, which is applied in the field of separation of beta-sitosterol and stigmasterol, can solve the problems of low yield and purity, high solvent toxicity, environmental pollution, etc., and achieves simple and fast process, improved product purity and yield. The effect of small rate and dosage

Inactive Publication Date: 2014-02-12
HUNAN KEYUAN BIO PRODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problems in these methods are that the yield and purity are not high, the process is complicated, or the solvent is highly toxic and pollutes the environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] This embodiment includes the following steps:

[0035] (1) Take 100 grams of mixed phytosterol, 16.6 grams of stigmasterol, and 300 grams of n-amyl alcohol, mix and heat to 62 ° C, stir evenly, heat filter to remove impurities, and obtain a filtrate;

[0036] The mixed phytosterols are refined sterols, and the total sterol mass fraction is 95.6%, wherein β-sitosterol is 45.4%, and stigmasterol is 30.2%; the stigmasterol is refined stigmasterol, and the purity is 95.7%;

[0037] (2) Cool the filtrate obtained in step (1) to 22°C, stir at a constant temperature for 2 hours, and heat-preserve and filter to obtain the filtrate and filter cake (45.5 g), the filter cake being crude stigmasterol;

[0038] (3) Continue cooling the filtrate obtained in step (2) to 5°C, add 10 grams of cold water (3°C), stir at a constant temperature for 2 hours, and filter with heat preservation to obtain 50.9 g of filter cake, which is crude β-sitosterol;

[0039] (4) Add the crude stigmastero...

Embodiment 2

[0042] This embodiment includes the following steps:

[0043] (1) Take 100 grams of mixed sterols, 22.9 grams of stigmasterol, and 310 grams of n-amyl alcohol, mix and heat to 68°C, stir evenly, heat filter to remove impurities, and obtain a filtrate;

[0044] The mixed phytosterols are refined sterols with a mass fraction of 96.3% of total sterols, including 50.5% of β-sitosterol and 28.5% of stigmasterol; the stigmasterol is refined stigmasterol with a purity of 97.4%;

[0045] (2) Cool the filtrate in step (1) to 20°C, stir at constant temperature for 6 hours, and heat-preserve and filter to obtain the filtrate and filter cake (50.6g), the filter cake is crude stigmasterol;

[0046] (3) Continue to cool down the filtrate in step (2) to 8°C, add 12 grams of cold water (3°C), stir at constant temperature for 3 hours, heat-preserve and filter to obtain 58.8g of filter cake, which is crude β-sitosterol;

[0047] (4) Add the crude stigmasterol obtained in step (2) into n-amyl a...

Embodiment 3

[0050] This embodiment includes the following steps:

[0051] (1) Take 100 grams of mixed phytosterol, 21.0 grams of stigmasterol, and 400 grams of n-amyl alcohol, mix and heat to 60°C, stir evenly, and heat filter to remove impurities to obtain a filtrate;

[0052] The mixed phytosterols are refined sterols, the total sterol mass fraction is 97.8%, wherein β-sitosterol is 55.5%, and stigmasterol is 35.3%; the stigmasterol is refined stigmasterol with a purity of 95.2%;

[0053] (2) Cool the filtrate in step (1) to 20°C, stir at constant temperature for 2 hours, and heat-preserve and filter to obtain the filtrate and filter cake (56.1 g), the filter cake is crude stigmasterol;

[0054] (3) Continue to cool down the filtrate in step (2) to 8°C, add 15 grams of cold water (3°C), stir at constant temperature for 3 hours, heat-preserve and filter to obtain 57.3g of filter cake, which is crude β-sitosterol;

[0055] (4) Add the crude stigmasterol obtained in step (2) into n-amyl a...

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Abstract

A method for separating beta-sitosterol and stigmasterol from mixed phytosterol comprises the following steps: (1) mixing and dissolving the mixed phytosterol and n-amyl alcohol at 60-70 DEG C, adding the stigmasterol to prepare a saturated solution of the stigmasterol at the temperature, and thermally filtering the solution to remove impurities so as to obtain a filter liquor; (2) cooling the filter liquor to 20-30 DEG C, stirring at a constant temperature, maintaining the temperature, and filtering to obtain a filter cake of crude stigmasterol; (3) cooling the filtrate to 0-10 DEG C, stirring at a constant temperature, maintaining the temperature, and filtering to obtain a filter cake of crude beta-sitosterol; (4) refining the crude stigmasterol with the n-amyl alcohol to obtain a stigmasterol product with high purity; (5) refining the crude beta-sitosterol with the n-amyl alcohol to obtain a beta-sitosterol product with high purity. The method is simple in process step and safe in production; the solvent is simplex, small in using amount, and easy to recycle; the products are high in purity and high in yield; the method is applicable to industrial production.

Description

technical field [0001] The invention relates to a method for separating β-sitosterol and stigmasterol from mixed phytosterols. Background technique [0002] Phytosterol is a natural active substance that exists in plants. It is a class of steroidal compounds with cyclopentane polyhydrophenanthrene as the main skeleton (steroid nucleus). More than 40 kinds of phytosterols have been confirmed, among which β-sitosterol, stigmasterol and campesterol are the main ones. They have the same chiral structure, and the only difference in structure is that they have side chains. It is these side chains The small differences in their biological functions lead to great differences in their physiological functions. [0003] The structures of different phytosterols are as follows: [0004] . [0005] Vegetable oil and oily foods are the main natural sources of phytosterols. The phytosterols extracted from vegetable oils and their by-products are a mixture, and further separation is re...

Claims

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Application Information

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IPC IPC(8): C07J9/00
Inventor 董士香
Owner HUNAN KEYUAN BIO PRODS
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