Method for separating beta-sitosterol and stigmasterol from mixed phytosterol

Abstract

A method for separating beta-sitosterol and stigmasterol from mixed phytosterol comprises the following steps: (1) mixing and dissolving the mixed phytosterol and n-amyl alcohol at 60-70 DEG C, adding the stigmasterol to prepare a saturated solution of the stigmasterol at the temperature, and thermally filtering the solution to remove impurities so as to obtain a filter liquor; (2) cooling the filter liquor to 20-30 DEG C, stirring at a constant temperature, maintaining the temperature, and filtering to obtain a filter cake of crude stigmasterol; (3) cooling the filtrate to 0-10 DEG C, stirring at a constant temperature, maintaining the temperature, and filtering to obtain a filter cake of crude beta-sitosterol; (4) refining the crude stigmasterol with the n-amyl alcohol to obtain a stigmasterol product with high purity; (5) refining the crude beta-sitosterol with the n-amyl alcohol to obtain a beta-sitosterol product with high purity. The method is simple in process step and safe in production; the solvent is simplex, small in using amount, and easy to recycle; the products are high in purity and high in yield; the method is applicable to industrial production.

Description

technical field

[0001] The invention relates to a method for separating β-sitosterol and stigmasterol from mixed phytosterols. Background technique

[0002] Phytosterol is a natural active substance that exists in plants. It is a class of steroidal compounds with cyclopentane polyhydrophenanthrene as the main skeleton (steroid nucleus). More than 40 kinds of phytosterols have been confirmed, among which β-sitosterol, stigmasterol and campesterol are the main ones. They have the same chiral structure, and the only difference in structure is that they have side chains. It is these side chains The small differences in their biological functions lead to great differences in their physiological functions.

[0003] The structures of different phytosterols are as follows:

[0004] .

[0005] Vegetable oil and oily foods are the main natural sources of phytosterols. The phytosterols extracted from vegetable oils and their by-products are a mixture, and further separation is re...