Compound microorganism bacterium agent for banana stem and leaf silage and preparation method of compound microorganism bacterium agent

A technology of banana stems and leaves and compound bacterial agents, which is applied in animal feed, animal feed, applications, etc., can solve the problems of low crude protein, low degradation rate, and restricted development of banana stems and leaves, and achieve the reduction of cellulose content and huge The effect of economic benefits

Inactive Publication Date: 2014-02-26
AGRI PROD PROCESSING INST GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The crude protein of banana stems and leaves is very low, which restricts the development of its deep processing
In addition, banana stems and leaves are rich in cellulose, hemicellulose and lignin, which are difficult to be

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Production of microbial compound bacteria agent

[0034] 1.1 Preparation of Lactobacillus plantarum freeze-dried inoculum

[0035] Lactobacillus plantarum NL4-10 was inoculated in MRS medium at 1% inoculum amount, and cultured at 37°C for 36 hours to a stable stage. Centrifuge at 10,000×g for 10 minutes at 4°C, remove the supernatant, wash the bacteria sludge twice with sterile phosphate buffer saline with a concentration of 60 mM and a pH value of 6.5, then centrifuge at 10,000×g for 10 minutes, and resuspend Add an equal volume of 10% (m / v) skim milk solution to 200 mL of sterile phosphate buffer as a protective agent, pre-freeze at -20°C for 2 hours, then pre-freeze at -80°C for 8 hours, and pre-freeze Then put it into a freeze dryer for freeze-drying, the cold trap temperature is -60°C, the vacuum degree is 4-6Pa, and the vacuum freeze-drying time is 16 hours, so that the Lactobacillus plantarum freeze-dried microbial agent can be obtained.

[0036] Among them,...

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PUM

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Abstract

The invention provides a compound microorganism bacterium agent for a banana stem and leaf silage and a preparation method of the compound microorganism bacterium agent. The preparation method comprises the following steps: A, preparing a lactobacillus plantarum freeze-dried bacterium agent; B, preparing freeze-dried candida utilis; C, preparing freeze-dried tannase; D, reviving the lactobacillus plantarum freeze-dried bacterium agent, the candida utilis and the tannase in sequence; E, fully stirring the obtained revived bacterium agents to prepare revived water, wherein the bacterium content of the revived water is kept at 10<8>-10<10> cfu/mL; F, cutting banana stems and leaves into pieces with size of 2-4 cm, processing until the water content is about 60-65 percent, uniformly stirring the revived water obtained in the step E and the banana stems and leaves, holding in a fermentation tank, compacting, sealing and keeping out of the sun at normal temperature to prepare the banana stem and leaf silage. By using the method, the waste material can be changed into things of value, tannin which goes against digestion in the banana stems and leaves can be eliminated and passivated, the cellulose content is reduced, the high-quality silage is developed, and great economic benefit is produced.

Description

technical field [0001] The invention relates to a composite bacterial agent in the field of microbial feed additives and a preparation method thereof, in particular to a microbial composite bacterial agent used in banana stem and leaf silage and a preparation method thereof. Background technique [0002] Silage is to put green (semi-dry) feed into the silo, and under anaerobic conditions, it is fermented by lactic acid bacteria to make feed that can be stored for a long time. This kind of feed can effectively preserve the nutrient components of green plants, and ensure the fresh and tender juice of the raw materials when they are green. Practice has proved that silage is an indispensable basic feed for scientific raising of livestock, especially for raising ruminant livestock. [0003] After the silage is fermented by anaerobic microorganisms, the nutrients are preserved, and the activities of microorganisms during the fermentation process can produce many small molecule nu...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/165A23K1/14A23K1/00
Inventor 游向荣李志春张雅媛付桂明孙健陆少峰刘成梅车江旅万茵李明娟李丽李昌宝黎昌炎卫萍
Owner AGRI PROD PROCESSING INST GUANGXI ACADEMY OF AGRI SCI
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