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Garlic saponin liposome and preparation method thereof

A technology of garlic saponins and glycosides, applied in the field of liposome preparations of garlic saponins and preparations thereof, can solve the problems that garlic saponins cannot be fully and effectively utilized and the like

Active Publication Date: 2014-02-26
山东荣群生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing literature has reported the extraction method of garlic saponin, but there are few reports on the preparation, development and utilization of garlic saponin formulations, so that the use of garlic saponin has not been fully and effectively utilized

Method used

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  • Garlic saponin liposome and preparation method thereof
  • Garlic saponin liposome and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 garlic saponin liposome

[0026] prescription:

[0027] Allicin

0.3g

Soy Lecithin

6.0g

cholesterol

2.0g

[0028] Preparation method (film dispersion method): Dissolve 6.0 soybean lecithin and 2.0g cholesterol in 20ml ether, and rotate to evaporate to form a film; dissolve 0.3g garlic saponin in about 5ml phosphate buffer (pH6.8), and The prepared solution was added to the above-mentioned film, and stood at 30°C for 30 minutes; added phosphate buffer solution to 300ml, and stirred at 30°C for 1.5h to obtain a garlic saponin liposome suspension. Each milliliter of suspension contains about 27.6mg of solid liposome. The garlic saponin liposome suspension can be freeze-dried to obtain solid garlic saponin liposome.

[0029] The garlic saponin liposome suspension was placed at 5° C. for 10 days, and no precipitation occurred. The encapsulation efficiency was determined to be 75.10% by ultrafiltrat...

Embodiment 2

[0030] The preparation of embodiment 2 garlic saponin liposome

[0031] prescription:

[0032] Allicin

0.2g

egg yolk lecithin

2.5g

cholesterol

1.1g

[0033] Preparation method (reverse evaporation method): Dissolve 2.5g of egg yolk lecithin and 1.1g of cholesterol in 20ml of ether, add 4ml of phosphate buffer (pH6.8) containing 0.2g of garlic saponin, and sonicate for 2min (every treatment is 0.5min , intermittent 0.5min), rotary evaporation to gel, vortex to invert the phase of the gel, continue rotary evaporation to remove ether, ultracentrifugation (10000r.min -1 Centrifuge for 60 min) to remove free garlic saponin, take the precipitate, add phosphate and dilute (pH6.8) (dilute to 300ml volume) to obtain garlic saponin liposome suspension. Each milliliter of the suspension contains about 12.6 mg of solid liposomes. The garlic saponin liposome suspension can be freeze-dried to obtain solid garlic saponin liposome.

[0034] The g...

Embodiment 3

[0035] The preparation of embodiment 3 garlic saponin liposome

[0036] prescription:

[0037] Allicin

0.2g

egg yolk lecithin

2.0g

cholesterol

1.6g

Tween 80

2.0g

Poloxamer F68

3.0g

[0038] Preparation method: 2.5g egg yolk phospholipids, 2.0g cholesterol were co-dissolved in 15ml ethanol, and rotatively evaporated in a water bath at 30°C to form a film; sequentially 3.0g poloxamer F68, 2.0g Tween 80, 0.2g garlic saponin Add 5ml of phosphate buffer solution (pH 6.8) to prepare the solution; add the solution to the above membrane, vortex and mix, add phosphate buffer solution (pH 6.8) to dilute to 300ml, and homogenize with magnetic stirring for 60min to obtain liposome suspension. Each milliliter of the suspension contains about 29.3 mg of solid liposomes. The garlic saponin liposome suspension can be freeze-dried to obtain solid garlic saponin liposome.

[0039] The garlic saponin liposome suspension was pl...

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Abstract

The invention provides a garlic saponin liposome, comprising the following components in parts by weight: 1 part of garlic saponin, 10-100 parts of phospholipid, 1-50 parts of cholesterol and 0-50 parts of emulsifier, wherein the garlic saponin liposome can be prepared by such conventional methods as film dispersion method, ultrasonic dispersion method and reverse evaporation method, for example, injection method, freeze drying method, etc. The garlic saponin liposome can be prepared to a plurality of formulations, for example, tablet, capsule, oral liquid, injection, aerosol or drop and the like, and the garlic saponin liposome is applied to preparation of drugs for preventing bacteria, reducing cholesterol, reducing blood fat, preventing platelet aggregation, improving fibrinolytic activity, preventing hypoxia and preventing oxidative damage, etc.

Description

technical field [0001] The invention relates to the field of pharmaceutical preparations, in particular to a liposome preparation of garlic saponin and a preparation method thereof. Background technique [0002] Garlic (garlic) is the underground bulb of the Liliaceae Allium plant garlic, which is currently planted all over the world. Garlic is an indispensable condiment in human daily life. It also has various physiological activities such as antibacterial, anti-inflammatory, insecticidal, blood fat lowering, blood pressure lowering, anti-rheumatism, anti-tumor, regulating body immunity and anti-oxidation. source of valuable resources. The composition of garlic is complex, mainly including sulfur-containing organic compounds, sugars, amino acids, enzymes, lipids, vitamins, trace elements, and saponins. Garlic products with different processing processes have different chemical components, so they have different effects and different degrees of safety. The bioactive compo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8962A23L1/29A61K8/97A61K9/127A61P31/04A61P3/06A61P35/00A61P7/02A61P39/00A61Q19/00A23L33/00
Inventor 宋兴良高振珅李振
Owner 山东荣群生物科技集团有限公司
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