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Purification and separation technology of crocin in gardenia extract

A technology of gardenia extract and crocin, applied in the field of medicine, can solve problems such as inability to obtain high-purity crocin, and achieve the effects of less impurities, low price and high product yield

Inactive Publication Date: 2014-02-26
WEIHAI NOVODA PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a new purification and separation technology aiming at the problems that high-purity crocin cannot be obtained in the prior art, that is, to utilize foam separation and macroporous resin adsorption technology, the process operation is simple, and the obtained saffron Glycosides have high purity, and the physical and chemical indicators meet the industry standards of food, medicine, cosmetics, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Dissolve 5g of gardenia extract in 3 times the amount of water, inject it into a foam separator, add ethyl acetate twice the amount of gardenia extract, and set the temperature at 45°C and 0.5Mpa to Feed nitrogen into the foam separator at a flow rate of 1.2L / h, separate the water layer in the foam separator after 2h-3h, and use the ethyl acetate layer for other purposes;

[0022] (2) Evaporate the water layer separated in the above steps to dryness under reduced pressure at 58°C and 0.07Mpa, and put the dried paste into 15% ethanol to fully dissolve;

[0023] (3) Connect four macroporous resin columns in series, and the columns contain four different macroporous resins, D101, AB-8, HPD500, and S-8, numbered 1, 2, 3, and 4 respectively; The ethanol solution in the above steps is injected into the macroporous resin column in series with a flow rate of 10ml / min, and after adsorption for 1.5h, the No. 1 column is eluted with 45% ethanol respectively to obtain the eluen...

Embodiment 2

[0027] (1) Dissolve 5g of gardenia extract in 3 times the amount of water, inject it into the foam separator, and then add ethyl acetate, which is 2 times the amount of gardenia extract, at 50°C and 0.6Mpa Feed nitrogen gas into the foam separator at a flow rate of 1.5L / h, and after 2h-3h, separate the water layer in the foam separator and the ethyl acetate layer for other use;

[0028] (2) Evaporate the water layer separated in the above steps to dryness under reduced pressure at 55°C and 0.08Mpa, and put the dried paste into 15% ethanol to fully dissolve;

[0029] (3) Connect four macroporous resin columns in series, and the columns contain four different macroporous resins, D101, AB-8, HPD500, and S-8, numbered 1, 2, 3, and 4; The ethanol solution in the step is injected into the macroporous resin column with a flow rate of 15ml / min, and after adsorption for 2h, the No. 1 column is eluted with 50% ethanol respectively to obtain the eluent of crocin-1; No. 2 column is elute...

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Abstract

The invention provides a purification and separation technology of crocin in a gardenia extract, namely a technology for adsorbing, purifying and separating crocin components by utilizing a foam separator and macroporous resin columns of different polarities. The technology comprises the following operation steps: adding an aqueous solution of the gardenia extract into the foam separator according to the differences in hydrophilcities and polarities of different components in crocin, adding ethyl acetate for extraction, concentrating the subnatant into dry paste, fully dissolving the dry paste in 15% ethanol, introducing the dissolution solution into four macroporous resins for adsorption, and performing gradient elute by using ethanol with different concentrations, thus purifying and separating crocin-1, crocin-2, crocin-3 and crocin-4. The selected raw materials are low in cost and readily available, the separation and purification process is simple and convenient to operate, each component of the purified crocin is high in purity and low in impurity content, and the quality performance index can reach the industrial standards of medicines, foods and cosmetics.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a technology for purifying and separating crocin in gardenia jasmine extracts. Background technique [0002] Crocin is mainly divided into crocin-1, glycoside-2, glycoside-3, and glycoside-4. In recent years, a large number of pharmacological studies have shown that crocin has great medicinal value. Currently, crocin The main effects of crocin are as follows: (1) Crocin can inhibit the increase of intracellular calcium ions, and has obvious calcium antagonism; (2) Scholars at home and abroad have conducted a large number of clinical studies on the lipid-lowering effect of crocin , studies have shown that crocin can significantly improve the liver function and blood environment of patients with fatty liver, and has a good therapeutic effect on fatty liver; (3) crocin can increase the activity of intracellular NOS and the content of cellular NO, thereby Protect endothelial cell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/06C07H1/08
Inventor 王河东张庆卫
Owner WEIHAI NOVODA PHARMA GRP
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