Monochamus alternates hope beauveria bassiana space mutant B305 and application thereof

A technology of Beauveria bassiana and Longhorn beauveria, applied in the directions of application, fungi, biocides, etc.

Active Publication Date: 2014-02-26
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The space environment has a good mutagenesis effect, which can produce beneficial mutations such as improving the beneficial traits of strains and

Method used

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  • Monochamus alternates hope beauveria bassiana space mutant B305 and application thereof
  • Monochamus alternates hope beauveria bassiana space mutant B305 and application thereof
  • Monochamus alternates hope beauveria bassiana space mutant B305 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0028] Experimental Example 1 Screening of Beauveria bassiana coccidioid mutagenic mutants and determination of mutagenic effect

[0029] 1 Experimental materials and methods

[0030] 1.1 Strains

[0031] Beauveria bassiana (Bals.) Vuill. (strain number cfcc81357) was isolated and purified from the larvae of Beauveria bassiana (Bals.) Vuill., which was infected with Beauveria bassiana from the masson pine trees in Lishui City, Jiangsu Province.

[0032] After the test Beauveria bassiana was returned to Earth through spaceflight mutagenesis, it was stored in the laboratory at 4°C for future use.

[0033] 1.2 Aerospace carrying

[0034] Beauveria bassiana (Bals.) Vuill. strain cfcc81357 was divided into two parts, one was kept on the ground for routine storage as a control (CK); the other was carried on November 1, 2011 " The Shenzhou 8 spacecraft lasted 6d20h. After returning to the ground, the materials were taken out in time and stored at low temperature.

[0035] 1.3 Mut...

experiment example 2

[0066] Experimental example 2 Determination of the pathogenicity of Beauveria bassiana aerospace mutant strains to monochamus larvae

[0067] 1. Experimental method

[0068] The larvae of Monochamus alternatus Hope were collected in Dalongshan Forest Farm, Anqing City, Anhui Province. The 4th instar larvae overwintered were used as the test material. They were reared indoors for 2 days, and the individuals with mechanical damage and abnormal behavior were excluded, and the healthy ones with basically the same size were selected. The larvae were used in the bioassay test

[0069] The spores of some strains were diluted to 1.0×10 7 cfu / mL -1 Concentration of spore suspension, with sterile water as a control. Dip the monochamus larvae in the spore suspension for 15 seconds, then place them in a sterile petri dish and let them crawl freely, so that there is no water drop on the surface of the body, and then place each monochamus in a 12 mm diameter × 75 mm high In a finger-sha...

experiment example 3

[0074] Experimental example 3 The application effect experiment of aerospace mutant strain B305 strain to control long beetle

[0075] 1. Test materials and methods

[0076] Select a number of pine sections with obvious invasion holes of Alpinia monochamus larvae from the forest, bring them back to the room, and divide them into 3 groups, with 10-15 sections in each group; Aerospace mutagenesis) to produce spore powder (3.0×10 10 / g), two control methods of sprinkling fungus powder on the surface of the wood section and injecting the fungus liquid into the invasion hole were used to carry out comparative tests. The amount of spore powder is based on the uniform distribution on the wood section, and the concentration of the bacterial solution injected into the entry hole is 1×10 7 1 / ml, 1ml per well (the amount of B305 strain and wild strain of Beauveria bassiana are equal). The control group did not receive any preventive treatment, and the control group was placed separate...

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Abstract

The invention discloses a monochamus alternates hope beauveria bassiana space mutant B305 and an application thereof. The mutant B305 with significant improvements in the aspects of growth speed, spore yield, pathogenicity to long-horned beetles and other performances is screened by enabling a beauveria bassiana wild strain to be carried on a Shenzhou-8 spacecraft to perform space induction and taking a non-carried original strain as a control, and the microorganism accession number is CGMCC No. 8049. Pathogenicity and indoor control experiments show that the pathogenicity and the control effect against monochamus alternatus hopes of the space mutant B305 are significantly higher than those of the original beauveria bassiana strain. The invention also discloses a biocontrol preparation prepared from the monochamus alternates hope bassiana space mutant B305. The invention further provides an application of the monochamus alternates hope beauveria bassiana space mutant B305 and a microbial preparation of the mutant B305 in the aspect of controlling long-horned beetles and other pests.

Description

technical field [0001] The present invention relates to entomopathogenic fungus mutants, in particular to Beauveria bassiana (Bals.) Vuill. aerospace mutants, and the present invention further relates to Beauveria bassiana (Bals.) Vuill. aerospace mutants The application in the control of long beetles belongs to the field of mutant strains of Beauveria bassiana and its application. Background technique [0002] Bine wood nematode disease caused by the pine wood nematode Bursaphelenchus xylophilus (Steiner&Buhrer) Nickle) is a devastating disease of pine trees, which seriously threatens forestry development. In East Asia, the disease is transmitted naturally by adult pine beetles (Monochamus alternates Hope) after eclosion, which spread to healthy pine trees during nutritional supplementation, resulting in the death of pine trees. Monochamus is distributed in China from Taiwan in the east, Guangdong in the south, Tibet in the west, Qinling in the northwest, Hebei in the nort...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 汪来发王曦茁刘洪剑董广平王源
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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