Bacillus amyloliquefaciens and control bacteria prepared by using it
A technology for the preparation of amylolytic spores and strains, applied in the field of biological control of tobacco diseases, can solve the problems that have not been reported and applied, and achieve the effect of beneficial prevention and ecological environment safety
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Embodiment 1
[0024] The Bacillus amyloliquefaciens CX-1 strain has been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on May 27, 2013. The address is: Institute of Microbiology, Chinese Academy of Sciences, No. 1, No. 1 Beichen West Road, Chaoyang District, Beijing, The deposit number is CGMCC No.7645.
[0025] The anti-bacterial agent for preventing and treating tobacco black shank prepared by utilizing the Bacillus amyloliquefaciens CX-1 strain is prepared by the following method steps:
[0026] (1) Strain preparation
[0027] Inoculate the Bacillus amyloliquefaciens CX-1 strain on the slant medium of the test tube, and cultivate it at a constant temperature of 32°C for 72 hours to obtain the test tube species. The slant medium formula is: glucose 10 g / L, peptone 5 g / L, beef extract 3 g / L, yeast extract 1 g / L, agar 18 g / L, pH6.8~7.0;
[0028] (2) Bacterial liquid fermentation
[0029] Add 10ml of sterile water to the t...
Embodiment 2
[0035] In order to test the control effect of the Bacillus amyloliquefaciens CX-1 bacterial strain provided by the present invention on tobacco black shank, the inventor under greenhouse conditions To understand the control experiment of Bacillus amylobacter CX-1 against tobacco black shank.
[0036] The tobacco test material used in the greenhouse experiment is variety K326, and the test site is the National Engineering Center of Yunnan Agricultural University. The soil used for potted tobacco seedlings in this experiment came from fields where tobacco black shank disease occurred heavily in previous years, that is, the pathogenic bacteria in the soil with bacteria was used to induce tobacco black shank disease. Those skilled in the art can also use the soil dilution method to isolate the pathogenic fungi from the soil according to the actual situation, and then reconnect the tobacco plants to make the tobacco plants disease.
[0037] Dilute the control bacteria agent of Ba...
Embodiment 3
[0053] In order to further verify the control effect of Bacillus amyloliquefaciens CX-1 bacterial strain on tobacco black shank, the inventor further carried out the control effect of Bacillus amyloliquefaciens CX-1 on tobacco black shank in Yiliang County, Yunnan Province. field test .
[0054] The test plot is a field where tobacco black shank disease occurs seriously all year round (the specific location is: N, 24°54′24.79″; E, 103°07′49.73″), and those skilled in the art can also use the soil according to the existing technology The pathogenic fungus was isolated from the field soil by the dilution method, and then the tobacco plants were inoculated to cause the disease. The experiment was carried out from April 6, 2012 to July 26, 2012.
[0055] On May 2, 2012, the tobacco seedlings were transplanted in the field, the spacing between plants and rows was 20cm×100cm, and the area of each experimental plot was 60m 2 , the cultivation conditions of all plots were the sam...
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