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A kind of preparation method of magnetic nanoprobe for diagnosing ovarian cancer

A magnetic nanometer and magnetic nanoparticle technology, applied in nanotechnology, instruments, measuring devices, etc., can solve the problems of rising and low sensitivity of ovarian cancer, and achieve the effect of rapid positioning

Inactive Publication Date: 2016-01-06
CHONGQING CANCER INST +1
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Problems solved by technology

80% of patients with advanced ovarian cancer have elevated CA-125 values, and the sensitivity to advanced ovarian cancer can reach 90%, but the sensitivity to early ovarian cancer is low, only 50-60%. Elevated in both dyslexia and inflammation

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  • A kind of preparation method of magnetic nanoprobe for diagnosing ovarian cancer
  • A kind of preparation method of magnetic nanoprobe for diagnosing ovarian cancer
  • A kind of preparation method of magnetic nanoprobe for diagnosing ovarian cancer

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Embodiment Construction

[0022] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0023] A method for preparing a magnetic nanoprobe for diagnosing ovarian cancer, the method comprising the steps of:

[0024] (1) Under alkaline conditions, magnetic nanoparticles were prepared by co-precipitation method, and they were modified with sodium citrate. The modified magnetic nanoparticles were reacted with silane coupling agent TEOS to prepare silanized Fe 3 o 4 Magnetic nanoparticles, namely Fe 3 o 4 SiO 2 .

[0025] (1.1) Fe 3 o 4 Synthesis and Characterization of Magnetic Nanoparticles

[0026] The classic chemical co-precipitation method is used to synthesize superparamagnetic fluid. The preparation principle is simple, mainly in the precipitant NH 3 .H 2 Under the action of O, divalent and ferric ions form precipitates under alkaline conditions, or use oxidation-reduction reactions to form Fe 3 o 4 .

[0...

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Abstract

The invention discloses a preparation method of a magnetic nanoprobe for ovarian cancer diagnosis. The preparation method comprises the following steps of 1, preparing and characterizing magnetic nanoparticles, 2, carrying out silanization and amination modification on the magnetic nanoparticles and characterization on the modified magnetic nanoparticles, and 3, carrying out glutaraldehyde activation on the magnetic nanoparticles subjected to silanization and amination modification, and crosslinking the activated magnetic nanoparticles and a CA125 monoclonal antibody to obtain the magnetic nanoprobe for ovarian cancer diagnosis. The invention also discloses a detection method for ovarian cancer diagnosis. The magnetic nanoprobe comprises the magnetic nanoparticles having small volumes so that more antigens or antibodies can be adsorbed; the magnetic nanoparticles comprise iron cores so that in a magnetic field, the magnetic nanoparticles have a fast sinking rate and can be washed and separated from solid carriers easily; and the magnetic nanoprobe can specifically capture CA125 in peripheral blood of ovarian cancer patients, an enriched sample is detected by the enzyme linked immunosorbent assay (ELISA), and the detection method for ovarian cancer early-warning and diagnosis based on the magnetic nanoprobe is built.

Description

technical field [0001] The invention relates to the field of preparation and biomedical application of a magnetic nano-probe, in particular to a method for preparing a magnetic nano-probe for tumor prevention and tumor marker CA125, which is used for early diagnosis of ovarian cancer. Background technique [0002] The mortality rate of ovarian cancer ranks first among gynecological malignancies, and there are almost no symptoms in the early stage. Due to the lack of effective early diagnosis and screening methods, 80% of ovarian cancers are diagnosed at an advanced stage, and the 5-year survival rate is only about 30%, while the 5-year survival rate of patients with early ovarian cancer (stage I, stage II) is as high as 90%. , The root of improving the overall survival rate of ovarian cancer lies in early detection. Therefore, finding effective early diagnosis and early warning methods is the fundamental way to improve the overall prognosis of ovarian cancer, reduce treatme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543B82Y40/00
CPCG01N33/54333G01N33/54346G01N33/57449
Inventor 周琦刘蕊夏之宁袁犁李丽仙徐发良郑晓东
Owner CHONGQING CANCER INST