Preparation method and purpose of total phenolic acid in pithecellobium clypearia

The technology of monkey earring and total phenolic acid, applied in the field of plant medicine, can solve the problem of no anti-tumor activity and the like, and achieve the effects of high drug availability, low pollution in the production process, and reasonable method design.

Inactive Publication Date: 2014-03-12
SHENYANG PHARMA UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, most of the research has focused on the anti-inflammatory and anti-viral aspects of monkey earrings, and there has been no report of anti-tumor activity.

Method used

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  • Preparation method and purpose of total phenolic acid in pithecellobium clypearia
  • Preparation method and purpose of total phenolic acid in pithecellobium clypearia
  • Preparation method and purpose of total phenolic acid in pithecellobium clypearia

Examples

Experimental program
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Effect test

Embodiment 1

[0015] The preparation of embodiment 1 monkey earring total phenolic acid

[0016] Take about 200g of monkey earrings, add 2000ml of water, heat and extract at 80°C for 2 hours, add 1000ml of water to the filtered residue and heat and extract at 80°C for 1 hour, combine the two filtrates, concentrate at 110°C and atmospheric pressure to 25°C, and measure the relative density of 1.25- 1.30 of the extract. Suspend this extract with 200ml of water and apply it to D101 macroporous resin, elute with acid water (PH=3) for 3 volumes, then elute with 40% ethanol-water until the effluent is nearly colorless, collect this part The eluate was recovered from the ethanol under reduced pressure, and spray-dried to obtain a dry powder to obtain 22.1 g of total phenolic acids, with a total yield of about 11.1%. Utilize spectrophotometric detection, its total phenolic acid content is 83%.

Embodiment 2

[0017] The preparation of the total phenolic acid of embodiment 2 monkey earrings

[0018] Take about 200g of monkey earrings, add 1600ml of pure water, heat and extract at 70°C for 2 hours, add 1000ml of pure water to the filter residue, heat and extract at 70°C for 1 hour, combine the two filtrates, concentrate at 110°C and atmospheric pressure to 25°C, and measure the relative density. 1.25-1.30 of the extract. Suspend this extract with 200ml of water and apply it to D312 macroporous resin, elute with acid water (PH=4) for 3 volumes, then elute with 50% ethanol-water until the effluent is nearly colorless, collect this part The eluate was recovered under reduced pressure to ethanol, spray dried to obtain a dry powder, and 20.3 g of total phenolic acids were obtained, with a total yield of about 10.2%. Utilize spectrophotometric method to detect, its phenolic acid content is 80%.

Embodiment 3

[0019] The preparation of the total phenolic acid of embodiment 3 monkey earrings

[0020] Take about 1,000g of monkey earrings, add 8,000ml of pure water, heat and extract at 90°C for 2 hours, add 3,000ml of pure water to the filter residue and heat and extract at 90°C for 1 hour, combine the two filtrates, concentrate at 105°C and atmospheric pressure to 25°C, and measure the relative density. 1.25-1.30 of the extract. Suspend this extract with 1000ml of water, apply AB-8 macroporous resin, elute with acid water (PH=3) for 3 volumes, and then elute with 40% ethanol-water until the effluent is nearly colorless, collect For this part of the eluate, ethanol was recovered under reduced pressure, and spray-dried to obtain a dry powder to obtain 98 g of total phenolic acids, with a total yield of about 9.8%. Utilize spectrophotometric method to detect, its phenolic acid content is 85%.

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Abstract

The invention provides a preparation method of total phenolic acid in a plant pithecellobium clypearia. The preparation method comprises the steps of crushing a pithecellobium clypearia raw material, extracting with pure water, condensing to form concrete, suspending with water, then putting on macroporous resin, eluting with acid water, removing water-soluble impurities, allowing phenolic acid substances to be maintained on the macroporous resin, eluting with a water-alcohol system to form a total phenolic acid extract which is also an effective part with pharmacological activity in the pithecellobium clypearia. The total phenolic acid in the pithecellobium clypearia has obvious antineoplastic activity, the pithecellobium clypearia, as traditional Chinese medicine, has lower toxic and side effects, and a new anti-tumor drug can be developed. The total phenolic acid in the pithecellobium clypearia can be prepared into various oral preparations. The method is reasonable in design and has the characteristics of simple technical process, low cost, low energy consumption and low pollution in a production process.

Description

technical field [0001] The invention relates to the technical field of plant medicine, in particular to a preparation method of total phenolic acid (extract) from plant monkey earrings and a new application in the preparation of antitumor drugs. Background technique [0002] Malignant tumors are major diseases that endanger human health and life. It is estimated that about 5 million people die of malignant tumor every year in the world, which is the second leading cause of death after cardiovascular disease. According to foreign sources, hundreds of new anti-cancer drugs have been developed all over the world, many of which are natural plant extracts. At present, there are about 130 to 150 kinds of anticancer drugs that have been approved for marketing in various countries around the world. There are about 1,300 to 1,500 kinds of anticancer drug preparations formulated with these drugs. In addition, there are more than 800 new anti-cancer drugs that are being studied but ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P35/00
Inventor 宋少江黄肖霄彭缨李玲芝刘庆博
Owner SHENYANG PHARMA UNIVERSITY
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