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A kind of extraction equipment of total flavonoids of fern

An extraction device and a technology for total flavonoids, which are applied in the field of extraction methods for total flavonoids from Pterocarpus and the extraction equipment using the method, can solve the problems of difficult removal of filter paper, poor sealing performance, poor filtering effect, etc., and achieve low extraction cost and increase Reduced activity, significant antioxidant effect

Active Publication Date: 2020-06-19
LANZHOU UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to explore the optimal extraction process of total flavonoids of Zeraria, and carry out preliminary research on the components of total flavonoids of Bracken and its antioxidant effect, and propose a method for extracting total flavonoids of Bracken and its antioxidant effect Determination has effectively improved the extraction rate of the total flavonoids of Bracken, and confirmed that the extract of Bracken has antioxidant properties. In order to solve the problems of poor effect and difficult removal of the filter paper after filtration, a decompression filtration device for extracting total flavonoids of Bracken was also proposed, which effectively improved the sealing of the device, and the filtration effect was better and the filter paper was easy to remove after the filtration work was completed. Down

Method used

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  • A kind of extraction equipment of total flavonoids of fern
  • A kind of extraction equipment of total flavonoids of fern
  • A kind of extraction equipment of total flavonoids of fern

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] See Figure 1-5 , A method for extracting total flavonoids from Potentilla anserina, comprising the following steps;

[0056] A method for extracting total flavonoids of Potentilla anserina, comprising the following steps;

[0057] S1. Take an appropriate amount of Potentilla tuber, wash the Potentilla tuber and put it in a drying oven at 30℃ to dry to constant weight, then crush the dried Potentilla tuber into Potentilla tuber powder and put it in a desiccator for use ;

[0058] S2. Weigh a certain amount of Potentilla tuber root powder and transfer it into a round-bottomed flask, then add ethanol solutions of different concentrations to prepare mixed solutions with different material-to-liquid ratios according to experimental needs, and then put the round-bottomed flask in a microwave oven and set a certain The microwave power, microwave temperature and microwave time of the same to obtain the extract;

[0059] S3. Take a part of the extract for treatment, and then use the ...

Embodiment 2

[0085] Based on Example 1 but different is;

[0086] Extraction of total flavonoids from Potentilla anserina

[0087] Wash the tubers of Potentilla anserina and put them in a drying oven at 30°C to dry them to constant weight. After crushing, put them in a desiccator for later use. Accurately weigh 5g of Potentilla tuber powder and transfer it into a round-bottomed flask, add ethanol solutions of different concentrations, extract for different periods of time at a certain microwave power and temperature, filter the extract under reduced pressure to remove the filter residue, transfer to a 100mL volumetric flask and use 60 Dilute% ethanol to the mark, shake well and set aside.

[0088] Single factor superior level analysis

[0089] Study ethanol concentration (0, 25, 50, 75, 100%, v / v), material-to-liquid ratio (1:10, 1:15, 1:20, 1:25, 1:30), microwave radiation time (1 , 2, 3, 4, 5min), microwave radiation temperature (40, 50, 60, 70, 80, 90 °C) on the influence of four factors on t...

Embodiment 3

[0098] Based on Examples 1 and 2 but different are:

[0099] DPPH method to determine antioxidant capacity

[0100] Dissolve the Potentilla angustifolia extract in 100μl of 50% methanol according to different masses, and mix it with 900μl of DPPH radical solution to make the concentrations respectively 3.125, 6.25, 12.5, 25, 50μg / mL, and use the same volume of 50% methanol as a blank Contrast. After being placed in the dark for 30 minutes, the absorbance was measured at 517 nm with a spectrophotometer. Calculate the scavenging capacity of DPPH by the following formula

[0101] Clearance rate (%)=(1-(A drug / A control))×100

[0102] ABTS+ method to determine antioxidant capacity

[0103] 2.0mmol / LABTS was added to 2.45mmol / L potassium persulfate solution for 4h at room temperature and protected from light to generate ABTS+ free radicals. ABTS+ was diluted with 0.1M sodium phosphate buffer (pH7.4), and the absorbance at 734nm was measured. Dissolve the Potentilla anserina extract in 5...

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Abstract

The invention discloses an extraction method for total flavone of potentilla anserine L. and extraction equipment utilizing the extraction method, and belongs to the technical field of total flavone extraction. According to the extraction method, the extraction rate is taken as a main inspection index, factors of an extraction agent, a solid-liquid ratio, extraction time, the extraction temperature and the like are considered, an extraction method for the total flavone of the potentilla anserine L. through microwave-assisted extraction is optimized, and the anti-oxygenation effect and components of the flavone of the potentilla anserine L. are studied primarily; the technology process is simple, the requirements for the production equipment and technology are low, the extraction cost is low, and the extraction efficiency of the total flavone is high; higher popularization and application value is achieved, and the flavone of the potentilla anserine L. has good in-vitro antioxidant activity and is a novel natural antioxidant or a functional food.

Description

Technical field [0001] The invention relates to the technical field of total flavonoids extraction, in particular to a method for extracting total flavonoids from Potentilla anserina and an extraction equipment applying the method. Background technique [0002] Potentiiia anserine (Potentiiia anserine L.) is the root tuber of Potentilla sibiricum, a plant belonging to the Rosaceae Potentilla genus. It was first published in "Commonly Used Chinese Medicinal Materials in Tibet". It is a commonly used Tibetan medicine with a history of more than 1,200 years. Food is eaten daily, and can also be used as medicine to treat diseases and strengthen the body. It has the effects of invigorating the spleen and stomach, promoting fluids and relieving coughs, replenishing qi and blood, nourishing yin and nourishing the kidneys. Kidney deficiency and qi deficiency, post-illness anemia, malnutrition and other symptoms. Potentilla is rich in carbohydrates, proteins, fatty acids, triterpenoids, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D11/02B01D29/085B01D29/58B01D29/96A61K36/73A61P39/06
CPCA61K36/73A61K2236/333A61P39/06B01D11/02B01D11/0288B01D29/085B01D29/58B01D29/96B01D2201/204B01D2201/34
Inventor 程菊刘迪赵丽霞沈蓉白德成
Owner LANZHOU UNIVERSITY
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