Surface acoustic micro-fluidic chip for tumor cell separation

A microfluidic chip and surface acoustic wave technology, applied in the field of cell separation, to achieve the effect of mature preparation process and low cost

Inactive Publication Date: 2014-03-26
河南省医药科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since the acoustic radiation force acting on the cells in the acoustic field is not only affected by the cell size, but also affected by the acoustic ratio factor determined by

Method used

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  • Surface acoustic micro-fluidic chip for tumor cell separation
  • Surface acoustic micro-fluidic chip for tumor cell separation
  • Surface acoustic micro-fluidic chip for tumor cell separation

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[0013] Such as figure 1 As shown, the surface acoustic wave microfluidic chip for tumor cell separation according to the present invention includes LiNbO 3 Substrate 1 and pasted on LiNbO by plasma bonding 3 The PDMS cover sheet 2 provided with microchannels on the substrate 1; wherein the microchannels include a horizontally arranged middle channel 3 with a width of 1 / 2 surface wave wavelength (λ), and the inlet 4 and the outlet 5 of the middle channel 3 respectively extend to the cover The upper surface of the sheet 2 is provided with a cell liquid injection channel 6 connected to the middle channel symmetrically on both sides of the entrance of the middle channel 3, and a cell collection channel 7 connected to the middle channel is symmetrically opened on both sides of the outlet of the middle channel 3 , The entrance of the cell fluid injection channel 6 and the exit of the cell collection channel 7 both extend to the upper surface of the cover sheet 2; deposits on the substr...

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Abstract

The invention discloses a surface acoustic micro-fluidic chip for tumor cell separation. The chip comprises a substrate, and a cover plate adhered on the substrate and provided with a micro-channel, wherein the micro-channel comprises a middle channel with an inlet and an outlet; cell sap injection channels connected with the inlet are arranged on two sides of the inlet; cell collection channels connected with the outlet are arranged on two sides of the outlet; two pairs of interdigital converters with identical frequencies and sound intensities are sequentially deposited on the substrate on two sides of the middle channel between the injection channel and the collection channel; the distance from the second pair of the interdigital converters to the middle channel is a quarter of surface wave length larger than that from the first pair of the interdigital converters to the middle channel; and small cell collection channels are symmetrically arranged on two sides of the middle channel between the two pairs of the interdigital converters. The chip has the advantages that a standing wave surface sound wave technology is realized, efficient, economic and pollution-free separation of tumor cell (CTCs) is realized, simultaneously the chip is low in cost, and the preparation technology is mature.

Description

technical field [0001] The invention relates to cell separation technology, in particular to a surface acoustic wave microfluidic chip for tumor cell separation. Background technique [0002] Malignant tumor is one of the major diseases that seriously endanger human health and has a high mortality rate. However, the formation of primary tumors is not the main cause of death of cancer patients. 90% of the deaths of cancer patients are caused by the metastasis of tumor lesions, which is mainly caused by the migration of tumor cells. The adhesion between tumor cells is very weak, and it is easy to fall off from the primary tumor. The shed tumor cells secrete a proteolytic enzyme, which destroys the connective tissue of the surrounding host and allows them to enter the peripheral blood circulation and become circulating tumor cells (Curculating Tumor Cells). , CTCs), the vast majority of tumor cells entering the circulation die within a short period of time, and a small number ...

Claims

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Application Information

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IPC IPC(8): C12M1/42
CPCC12M47/04
Inventor 张丽果汲振余赫培远赵立群郑立运欧阳军张壮丽
Owner 河南省医药科学研究院
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