Surface acoustic micro-fluidic chip for tumor cell separation

Abstract

The invention discloses a surface acoustic micro-fluidic chip for tumor cell separation. The chip comprises a substrate, and a cover plate adhered on the substrate and provided with a micro-channel, wherein the micro-channel comprises a middle channel with an inlet and an outlet; cell sap injection channels connected with the inlet are arranged on two sides of the inlet; cell collection channels connected with the outlet are arranged on two sides of the outlet; two pairs of interdigital converters with identical frequencies and sound intensities are sequentially deposited on the substrate on two sides of the middle channel between the injection channel and the collection channel; the distance from the second pair of the interdigital converters to the middle channel is a quarter of surface wave length larger than that from the first pair of the interdigital converters to the middle channel; and small cell collection channels are symmetrically arranged on two sides of the middle channel between the two pairs of the interdigital converters. The chip has the advantages that a standing wave surface sound wave technology is realized, efficient, economic and pollution-free separation of tumor cell (CTCs) is realized, simultaneously the chip is low in cost, and the preparation technology is mature.

Description

technical field [0001] The invention relates to cell separation technology, in particular to a surface acoustic wave microfluidic chip for tumor cell separation. Background technique [0002] Malignant tumor is one of the major diseases that seriously endanger human health and has a high mortality rate. However, the formation of primary tumors is not the main cause of death of cancer patients. 90% of the deaths of cancer patients are caused by the metastasis of tumor lesions, which is mainly caused by the migration of tumor cells. The adhesion between tumor cells is very weak, and it is easy to fall off from the primary tumor. The shed tumor cells secrete a proteolytic enzyme, which destroys the connective tissue of the surrounding host and allows them to enter the peripheral blood circulation and become circulating tumor cells (Curculating Tumor Cells). , CTCs), the vast majority of tumor cells entering the circulation die within a short period of time, and a small number ...

AI Technical Summary

Problems solved by technology

Since the acoustic radiation force acting on the cells in the acoustic field is not only affected by the cell size, but also affected by the acoustic ratio factor determined by the cell density and compressibility, so far, no one has made a surface acoustic wave-based microflow Application of controlled separation technology to the separation of CTCs

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