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Compound synergism method of enterococcus faecalis and application thereof

A technology of Enterococcus faecalis and Enterococcus faecium, applied in the field of microbial technology application, can solve problems such as unsatisfactory use effect, low survival rate of viable bacteria, unstable product properties, etc., and achieve improved water quality environment, high bacterial survival rate, The effect of water quality stabilization is obvious

Active Publication Date: 2014-03-26
湖北至正天辰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It solves the problems of low survival rate of live bacteria, unstable product properties and unsatisfactory use effect in the current Enterococcus faecalis preparations on the market

Method used

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  • Compound synergism method of enterococcus faecalis and application thereof
  • Compound synergism method of enterococcus faecalis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Example 2 Example 3 Example 4 Example 5 Enterococcus faecalis 2 4 6 8 10 Enterococcus faecium 8 6 4 2 1 Streptococcus acidophilus 8 6 4 2 1 Saccharomyces cerevisiae 2 3 4 5 6 lactose 0.2 0.5 1 2 2.5 fructooligosaccharide 2.5 2 1 0.5 0.2 Chitosan 0.2 0.5 1 2 2.5 yeast 0.6 1.2 1.8 2 2.4 folic acid 0.2 0.5 1 2 2.5 citric acid 2.5 2 1 0.5 0.2

[0031] A method for compounding and synergizing Enterococcus faecalis and its application, the product preparation steps are:

[0032] a. Strains: Enterococcus faecalis, Enterococcus faecium, Streptococcus acidophilus, Saccharomyces cerevisiae.

[0033] b. Production of powdery composite microbial agent: mix Enterococcus faecalis, Enterococcus faecium, Streptococcus acidophilus, and Saccharomyces cerevisiae (the fineness of the bacterial powder is greater than 100 mesh) according to the above ratio to ...

Embodiment 6

[0036] Embodiment 6: A method for compounding and synergizing Enterococcus faecalis and its application, the product preparation steps are:

[0037]Weigh 4 to 5 parts by weight of powdery composite microbial agent, 5 to 6 parts by weight of live bacteria growth accelerator, the above materials are thoroughly mixed, dried at a low temperature of 40°C, cooled, and passed through a 40-mesh sieve. Bacteria packaging is made into a composite microbial agent, and the effective number of viable bacteria in the agent is greater than 10 billion / g.

[0038] The powdery composite microbial agent is Enterococcus faecalis, Enterococcus faecium, Streptococcus acidophilus, and Saccharomyces cerevisiae, according to 1-10 parts: 1-8 parts: 1-8 parts: 1-6 parts It is made by mixing the parts by weight ratio. The live bacteria reproduction accelerator includes lactose, fructooligosaccharide, chitosan, yeast powder, folic acid, citric acid, etc., according to 0.1-2.5 parts: 0.1-2.5 parts: 0.1-...

Embodiment 7

[0039] Embodiment 7: A method for compounding and synergizing Enterococcus faecalis and its application, compared with other enterococcus faecalis preparations in the market, the steps are:

[0040] The organic matter decomposition ability, protease, amylase hydrolysis ability, growth rate and other performances of this Enterococcus faecalis preparation product and other enterococcus faecalis preparation products on the market were compared. The main results are as follows:

[0041] Table 1: Performance comparison table between the Enterococcus faecalis preparation of the present invention and the Enterococcus faecalis products on the market

[0042]

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PUM

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Abstract

The invention relates to a compound synergism method of enterococcus faecalis and application thereof. The compound synergism method of enterococcus faecalis mainly comprises microbe strain compounding, viable bacteria breeding promoter compounding and the like, wherein the microbe strain is mainly composed of one or more of enterococcus faecalis, enterococcus faecium, acidophilic streptococcus and saccharomycetes in a mixing manner; the viable bacteria breeding promoter is mainly composed of one or more of xylitol, lactose, fructo-oligose, xylooligosaccharide, chitosan, yeast powder, folic acid and citric acid in the mixing manner. By adopting the compound synergism method of the bacterial strain disclosed by the invention, the synergistic effects of different microbe strains are achieved, the purification process of a water pollutant is accelerated, and balance of a material flow and an energy flow of an aquacultural ecosystem is facilitated. The product is used in the aquaculture process, so that a bacteria phase and an algae phase of the water can be effectively balanced, the pollutants such as residual feeds, faeces, animal carcass and the like are quickly decomposed, the aquaculture water quality is improved, the stability of the aquaculture water is increased, and ageing of the aquaculture water is prevented.

Description

technical field [0001] The invention belongs to the field of microbial technology application, and in particular relates to a compounding and synergizing method of Enterococcus faecalis, and also relates to the application of a compound microbial bacterial agent of Enterococcus faecalis in aquaculture. Background technique [0002] Enterococcus faecalis is also called streptococcus faecalis, its shape is streptococcus or spherical, the diameter of the bacteria is between 0.3 μm and 0.7 μm, without spores, the colony formed on the broth agar plate after dilution is round, milky white, the surface The bumps are moist, shiny, with neat edges, most of them are arranged in pairs or short chains, and usually do not move. Enterococcus faecalis is a representative species of enterococci in group D streptococci. Usually, the so-called Enterococcus faecalis mainly includes Streptococcus faecalis, Streptococcus faecalis liquefaction subspecies, Streptococcus faecalis enzyme-producing s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C02F3/34C12R1/865C12R1/46C12R1/01
Inventor 荣克明张学振冯宪斌
Owner 湖北至正天辰生物科技有限公司
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