Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation and application method for treating swine viral diarrhea biological agent

A technology for porcine viral diarrhea and biological preparations, applied in antiviral agents, antiviral immunoglobulins, drug combinations, etc., can solve the problems of inconclusive damage to livestock and poultry, no way to remove them, etc., to achieve no damage to antibody activity, no Pollution, the effect of improving purification efficiency

Inactive Publication Date: 2014-04-02
刘聚祥
View PDF3 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, polyethylene glycol is easily soluble in water, and there is no way to remove it in the obtained supernatant, and the harm to livestock and poultry has not yet been determined.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation and application method for treating swine viral diarrhea biological agent
  • Preparation and application method for treating swine viral diarrhea biological agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] A preparation and application method of a biological agent for treating porcine viral diarrhea, specifically comprising the following steps:

[0025] (1) Preparation of immunogen preparation: immunogen is extracted protein, cell supernatant, vaccine, etc., and needs to contain characteristic antigens to stimulate the body to produce specific antibodies. What is used in the present invention is porcine transmissible gastroenteritis porcine epidemic diarrhea Double inactivated vaccine, the vaccine contains two kinds of viral proteins, which can stimulate the body to produce corresponding antibodies and transfer them to the egg yolk.

[0026] (2) Immunization procedure: the initial immunization dose was 1mL, multi-point subcutaneous injection in the chest; the second immunization, the third immunization and the fourth immunization were performed at intervals of two weeks, and the immunization doses were 1ml, 1.5ml, 1ml, 4 Booster immunization 28 days after immunization, th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation and application method for treating a swine viral diarrhea biological agent, and belongs to the technical field of biological agents. The method specifically comprises the following steps of: (1) preparing for immunogen preparation, namely, getting swine transmissible gastroenteritis and porcine epizootic diarrhea duplex inactivated vaccine which consists of two viral protein; (2) performing immune procedure; (3) detecting valence of antibody; (4) purifying an egg yolk antibody; and (5) identifying the egg yolk antibody. The preparation and application method has the advantages that the water diluting method is performed and the method is safe, reliable, out of pollution, and low in cost; the treated high-immunity egg can gain the egg yolk antibody with high activity, purity and output and stable performance; the operation method is simple, feasible, and reliable; the industrial production can be carried out conveniently.

Description

technical field [0001] The invention relates to a preparation and application method of a biological preparation for treating porcine viral diarrhea, belonging to the technical field of biological preparations. Background technique [0002] Porcine transmissible gastroenteritis (TGE) and porcine epidemic diarrhea (PED) are two infectious enterovirus diseases that cause diarrhea in pigs, both of which are members of the genus Coronaviridae. Due to the rapid spread of these two diseases and the high fatality rate caused by them, they can become outbreaks in epidemic seasons and cause significant economic losses to the pig industry. They are currently the key diseases in the prevention and control of pig diseases in my country. At present, vaccination is still the main measure to prevent the disease. There is no effective drug for treatment. Once the disease occurs, the losses are often heavy. With the gradual banning of some domestic antibiotics and the emergence of drug resis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K16/10C07K16/02A61K39/42A61P1/12A61P31/14
Inventor 刘聚祥曹立辉高岭
Owner 刘聚祥
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com