Preparation and application method for treating swine viral diarrhea biological agent

A technology for porcine viral diarrhea and biological preparations, applied in antiviral agents, antiviral immunoglobulins, drug combinations, etc., can solve the problems of inconclusive damage to livestock and poultry, no way to remove them, etc., to achieve no damage to antibody activity, no Pollution, the effect of improving purification efficiency

Inactive Publication Date: 2014-04-02
刘聚祥
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  • Preparation and application method for treating swine viral diarrhea biological agent
  • Preparation and application method for treating swine viral diarrhea biological agent

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Embodiment

[0024] A preparation and application method of a biological agent for treating porcine viral diarrhea, specifically comprising the following steps:

[0025] (1) Preparation of immunogen preparation: immunogen is extracted protein, cell supernatant, vaccine, etc., and needs to contain characteristic antigens to stimulate the body to produce specific antibodies. What is used in the present invention is porcine transmissible gastroenteritis porcine epidemic diarrhea Double inactivated vaccine, the vaccine contains two kinds of viral proteins, which can stimulate the body to produce corresponding antibodies and transfer them to the egg yolk.

[0026] (2) Immunization procedure: the initial immunization dose was 1mL, multi-point subcutaneous injection in the chest; the second immunization, the third immunization and the fourth immunization were performed at intervals of two weeks, and the immunization doses were 1ml, 1.5ml, 1ml, 4 Booster immunization 28 days after immunization, th...

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Abstract

The invention relates to a preparation and application method for treating a swine viral diarrhea biological agent, and belongs to the technical field of biological agents. The method specifically comprises the following steps of: (1) preparing for immunogen preparation, namely, getting swine transmissible gastroenteritis and porcine epizootic diarrhea duplex inactivated vaccine which consists of two viral protein; (2) performing immune procedure; (3) detecting valence of antibody; (4) purifying an egg yolk antibody; and (5) identifying the egg yolk antibody. The preparation and application method has the advantages that the water diluting method is performed and the method is safe, reliable, out of pollution, and low in cost; the treated high-immunity egg can gain the egg yolk antibody with high activity, purity and output and stable performance; the operation method is simple, feasible, and reliable; the industrial production can be carried out conveniently.

Description

technical field [0001] The invention relates to a preparation and application method of a biological preparation for treating porcine viral diarrhea, belonging to the technical field of biological preparations. Background technique [0002] Porcine transmissible gastroenteritis (TGE) and porcine epidemic diarrhea (PED) are two infectious enterovirus diseases that cause diarrhea in pigs, both of which are members of the genus Coronaviridae. Due to the rapid spread of these two diseases and the high fatality rate caused by them, they can become outbreaks in epidemic seasons and cause significant economic losses to the pig industry. They are currently the key diseases in the prevention and control of pig diseases in my country. At present, vaccination is still the main measure to prevent the disease. There is no effective drug for treatment. Once the disease occurs, the losses are often heavy. With the gradual banning of some domestic antibiotics and the emergence of drug resis...

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Application Information

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IPC IPC(8): C07K16/10C07K16/02A61K39/42A61P1/12A61P31/14
Inventor 刘聚祥曹立辉高岭
Owner 刘聚祥
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