Infantile seven-element tea preparation medium-polarity component fingerprint detection method and construction method
A technology of children's seven-star tea and fingerprints, which is applied in the direction of measuring devices, material separation, and analysis of materials, to achieve the effects of high precision, good reproducibility, and quality monitoring
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Embodiment 1
[0034] Construction of fingerprints of middle polar components in Xiaoer Qixing tea
[0035] 1 Instruments and equipment
[0036] Shimadzu LC-20AD high performance liquid chromatography (Shimadzu Corporation);
[0037] Sartorius CP225D analytical balance (d=0.01mg) (Germany Sartorius company);
[0038] Ratavapor R-Ⅱ rotary evaporator (Buchi, Switzerland);
[0039] SHZ-D(Ⅲ) Circulating water positive air pump (Gongyi Yuhua Instrument Co., Ltd.);
[0040] RTHW electric heating mantle (Gongyi City Yuhua Instrument Co., Ltd.);
[0041] SK-1 fast mixer (Jiangsu Jintan Jiamei Instrument Factory);
[0042] 2 Materials and reagents
[0043] Pediatric Qixing tea granule sample (provided by Guangzhou Wanglaoji Pharmaceutical Co., Ltd.);
[0044] 3 kinds of flavonoid reference substances: Liquiritin, Chlorogenic Acid, Apigenin (National Institute for the Control of Pharmaceutical and Biological Products);
[0045] Ethanol (analytical grade, Tianjin Zhiyuan Chemical Reagent Co., Lt...
Embodiment 2
[0078] Detection of Xiaoer Qixing Tea by Fingerprint of Middle Polar Components in Xiaoer Qixing Tea
[0079] 1 Instruments and equipment
[0080] With embodiment 1.
[0081] 2 Materials and reagents
[0082] Another 10 batches of Xiaoer Qixing tea granule samples (provided by Guangzhou Wanglaoji Pharmaceutical Co., Ltd.), and other materials and reagents are the same as in Example 1.
[0083] 3 methods
[0084] 3.1 Preparation of reference solution
[0085] With embodiment 1.
[0086] 3.2 Pretreatment of AB-8 macroporous resin
[0087] With embodiment 1.
[0088] 3.3 Preparation of the test solution
[0089] With embodiment 1.
[0090] 3.4 Chromatographic conditions
[0091] With embodiment 1.
[0092] Determination: Inject 10 μL of the reference substance solution and the test solution respectively, inject them into a high-performance liquid chromatograph, and measure each chromatogram.
[0093] 10 batches of Xiaoer Qixing tea preparation samples were detected by ...
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