Methods of detecting changes in biomolecules and methods of detecting changes in biomodulatory molecules
A biomolecular and molecular technology, applied in the field of detecting changes in biological regulatory molecules, and detecting changes in biomolecules, can solve the problems of difficulty in directly measuring the regulatory process.
Active Publication Date: 2017-02-15
ACAD OF MATHEMATICS & SYSTEMS SCIENCE - CHINESE ACAD OF SCI
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Problems solved by technology
At present, it is very difficult to directly measure the regulatory process. It is a very challenging computational biology and bioinformatics problem to accurately infer the regulatory mechanism under the generalized central dogma by using information on the binding strength of regulatory molecules such as transcription factors or microRNA to DNA.
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Embodiment 1
[0061] Example 1: Studying the molecular biological mechanism of a diet rich in polyunsaturated fatty acids from the sea to improve health through the small intestinal gene expression profile of mice.
[0062] 1. Sample preparation:
[0063] 4-month-old male mice (C57BL / 6J strain) were divided into two groups and fed with two high-fat diets for 4 weeks. The diet of the control group used linseed oil as the only fat source, and linseed oil is rich in α-linoleic acid, abbreviated as ALA (alpha linoleic acid). The food structure of the treatment group was consistent with that of the control group, the difference being that 44% of the lipid components were polyunsaturated fatty acids from the sea, including 6% of eicosapentaenoic acid (EPA) and 51% of long-chain docosahexaenoic acid (DHA). The treatment group is referred to simply as the PUFA group. After 4 weeks, the mice were sacrificed, and the small intestine was cut from 3 cm below the stomach to the cecum, and then washed...
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The invention discloses a method for detecting change of biological molecules. The method includes the steps of firstly, measuring a processed sample and a comparison sample through a biological chip or high-flux sequencing to obtain processed data and comparison data respectively; secondly, normalizing the processed data through the comparison data to obtain unbiased genetic expression different values, wherein a linear sample strip model is set up between the processed data and the corresponding comparison data in the normalization process, parameters of the linear sample strip model are estimated through a stable statistic estimation method, and values in the processed data are corrected through the linear sample strip model with the parameters and serve as the values obtained after the normalization. The invention further provides a method for detecting the quantitative index of the change of biological regulation molecules through integration of the genetic expression difference values and through combination strength of the biological regulation molecules and corresponding genes. By means of the methods, useful information in high-flux expression data can be effectively excavated, and a regulation mechanism of the genetic expression difference is determined.
Description
technical field [0001] The invention relates to the field of biomedicine, in particular to a method for detecting changes in biomolecules and a method for detecting changes in bioregulatory molecules. Background technique [0002] The process from DNA to protein is called gene expression, and the regulation of this process is called regulation of gene expression or gene control. Gene regulation is one of the central topics of modern molecular biology research. Because in order to understand the growth and development laws, morphological and structural characteristics, and biological functions of animals and plants, it is necessary to understand the time and space concepts of gene expression regulation. Mastering the mechanism of gene regulation is equivalent to mastering a key to reveal the mysteries of biology. [0003] Measuring genome-wide expression values in cell samples, tissue samples, etc. is a primary problem in functional genomics. Current measurement technolog...
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IPC IPC(8): G06F19/20
Inventor 李雷王琳
Owner ACAD OF MATHEMATICS & SYSTEMS SCIENCE - CHINESE ACAD OF SCI