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Preparation method and application of deproteinized tobacco extract

A technology for tobacco extract and deproteinization, which is applied in the field of preparation of deproteinized tobacco extract, can solve the problems of long time, difficult control and high technical requirements, and achieves the effect of improving aroma quality and aroma quantity and improving quality

Active Publication Date: 2014-04-23
CHINA TOBACCO GUANGDONG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can reduce the content of starch, protein, cellulose, lignin and pectin in tobacco extract, and has relatively high technical requirements. Compared with traditional natural enzyme molecules, immobilized enzymes are relatively more fragile and difficult to control, and This method takes a long time and has certain limitations on the scope of use of tobacco extracts.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of deproteinized tobacco extract in this embodiment includes the following steps:

[0030] S1. Adjust the temperature of the oven to 50°C, put 200 grams of tobacco shreds into the oven and dry it to a moisture content of 7%, then crush the dried tobacco shreds with a pulverizer and pass through a 100-mesh sieve. Get tobacco powder;

[0031] S2. Mix the tobacco powder and water in step S1 at a ratio of 1:10, stir with an automatic mixer for 1 hour, and add NaOH to adjust the pH to 10;

[0032] S3. Adjust the rotation speed of the centrifuge to 5000rpm, and use a centrifuge to separate the mixture in step S2, and the centrifugation time is 20min to obtain precipitation A and supernatant A;

[0033] S4. Adjust the pH of the supernatant A in step S3 to 5 with citric acid, adjust the speed of the centrifuge to 5000 rpm, and separate the pH-adjusted supernatant A with a centrifuge. The centrifugation time is 20 minutes to obtain a precipitate B and supernatant...

Embodiment 2

[0037] The preparation method of deproteinized tobacco extract in this embodiment includes the following steps:

[0038] S1. Adjust the temperature of the oven to 60°C, put 200 grams of tobacco shreds into the oven, and dry until the moisture content is 4%, and then crush the dried tobacco shreds with a pulverizer and pass through a 100-mesh sieve. Get tobacco powder;

[0039] S2. Mix the tobacco powder and water in step S1 at a ratio of 1:10, stir with an automatic mixer for 1 hour, and add NaOH to adjust the pH to 8;

[0040] S3. Adjust the rotation speed of the centrifuge to 5000rpm, and use a centrifuge to separate the mixture in step S2, and the centrifugation time is 20min to obtain precipitation A and supernatant A;

[0041] S4. Adjust the pH of the supernatant A in step S3 to 3 with citric acid, adjust the speed of the centrifuge to 5000 rpm, and use the centrifuge to separate the pH-adjusted supernatant A. The centrifugation time is 20 minutes to obtain a precipitate B and s...

Embodiment 3

[0045] The preparation method of deproteinized tobacco extract in this embodiment includes the following steps:

[0046] S1. Adjust the temperature of the oven to 55°C, put 200 grams of tobacco shreds into the oven and dry it to a moisture content of 6%, then crush the dried tobacco shreds with a grinder and pass through a 100-mesh sieve. Get tobacco powder;

[0047] S2. Mix the tobacco powder and water in step S1 at a ratio of 1:10, stir with an automatic mixer for 1 hour, and add NaOH to adjust the pH to 9;

[0048] S3. Adjust the rotating speed of the centrifuge to 3000 rpm, and use a centrifuge to separate the mixture in step S2 for a centrifugation time of 20 minutes to obtain a precipitate A and a supernatant A;

[0049] S4. Adjust the pH of the supernatant A in step S3 to 4 with citric acid, adjust the speed of the centrifuge to 3000 rpm, and use the centrifuge to separate the pH-adjusted supernatant A. The centrifugation time is 20 minutes to obtain a precipitate B and supern...

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PUM

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Abstract

The invention relates to the technical field of preparation of tobacco extracts, and discloses a preparation method and application of a deproteinized tobacco extract. The method comprises the following steps: smashing chopped tobacco into tobacco powder; mixing the tobacco powder with water, and adding alkali for regulating the pH to 8-10; separating the mixture to obtain a precipitate A and a supernate A; regulating the pH of the supernate to 3-5, separating to obtain a precipitate B and a supernate B, and removing the precipitate B; concentrating the supernate B, mixing with the precipitate A, and adding alkali for regulating the pH to 7-8 to obtain the deproteinized tobacco extract. By adopting preparation method of the deproteinized tobacco extract, proteins in tobacco can be removed completely, and the tobacco extract with low protein content and a good flavoring effect can be obtained. By adding the deproteinized tobacco extract prepared by using the preparation method into cut tobacco in the mass ratio of 0.2-2 percent, the flavor quality and flavor amount of prepared cigarettes can be enhanced greatly while irritation to the mouth and throat is not increased.

Description

Technical field [0001] The present invention relates to the technical field of preparing tobacco extracts, and more specifically, to a preparation method and application of deproteinized tobacco extracts. Background technique [0002] Tobacco extracts are mostly prepared by using tobacco shreds and shreds as raw materials. They play an irreplaceable role in increasing the natural tobacco aroma and the aroma of old tobacco, increasing the concentration of smoke, and harmonizing with tobacco aroma. Whether it is directly applied to cigarettes or the blending of other flavor-based products, there are a large number of applications. Many tobacco flavor manufacturers and even cigarette companies have developed various tobacco extracts with different raw materials and different extraction processes. [0003] Tobacco protein gradually accumulates with the growth and development of tobacco. As an edible protein resource, tobacco protein has a positive effect, but its impact on the smoking...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/18A24B15/24
Inventor 叶荣飞赵瑞峰饶国华
Owner CHINA TOBACCO GUANGDONG IND
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