Mouse nerve growth factor-containing expression vector and cell

A nerve growth factor and cell technology, applied in the direction of nerve growth factor, growth factor/inducing factor, cells modified by introducing foreign genetic material, etc., can solve the problems of low expression, low activity, reduced biological activity, etc. The sequence is correct, the expression is high, and the activity is good.
CN103740754AActive Publication Date: 2014-04-23NAT INST FOR FOOD & DRUG CONTROL
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NAT INST FOR FOOD & DRUG CONTROL
Publication Date
2014-04-23

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Abstract

The present invention relates to an expression vector for expressing a growth factor and a cell, specifically, relates to a mouse nerve growth factor-containing recombinant vector and a recombinant cell, and also relates to a preparation method of the recombinant cell, a purification method of a mouse nerve growth factor and an application of the recombinant vector or the recombinant cell in preparation of the mouse nerve growth factor. The mouse nerve growth factor is successfully expressed by utilizing a mammalian cell expression system, and the mouse nerve growth factor is further proved to have correct sequence and higher specific activity, and has a good application prospect.
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Description

technical field

[0001] The present invention relates to expression vectors and cells for expressing growth factors. Specifically, the present invention relates to recombinant vectors and recombinant cells containing mouse nerve growth factor. The present invention also relates to the preparation method of said recombinant cells and the purification of mouse nerve growth factor. The method and the use of the recombinant vector or the recombinant cell for preparing mouse nerve growth factor. Background technique

[0002] Nerve growth factor, NGF for short, is a secreted protein belonging to the neurotrophic factor family, which plays an important role in maintaining the survival of neurons and promoting their growth. NGF was first discovered by Rita Monterange in 1952. It was originally isolated from snake venom. Later, it was found that NGF is rich in rat submandibular gland and has a high specific activity. The process of extracting NGF from rat submandibular gland is relati...

Claims

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